Theoretical and Applied Genetics

, Volume 111, Issue 8, pp 1514–1523 | Cite as

Construction of an oilseed rape (Brassica napus L.) genetic map with SSR markers

  • J. Piquemal
  • E. Cinquin
  • F. Couton
  • C. Rondeau
  • E. Seignoret
  • I. doucet
  • D. Perret
  • M.-J. Villeger
  • P. Vincourt
  • P. Blanchard
Original Paper

Abstract

We constructed a Brassica napus genetic map with 240 simple sequence repeats (SSR) primer pairs from private and public origins. SSR, or microsatellites, are highly polymorphic and efficient markers for the analysis of plant genomes. Our selection of primer pairs corresponded to 305 genetic loci that we were able to map. In addition, we also used 52 sequence-characterized amplified region primer pairs corresponding to 58 loci that were developed in our lab. Genotyping was performed on six F2 populations, corresponding to a total of 574 F2 individual plants, obtained according to an unbalanced diallel cross design involving six parental lines. The resulting consensus map presented 19 linkage groups ranging from 46.2 to 276.5 cM, which we were able to name after the B. napus map available at http://ukcrop.net/perl/ace/search/BrassicaDB, thus enabling the identification of the A genome linkage groups originating from the B. rapa ancestor and the C genome linkage groups originating from the B. oleracea ancestor in the amphidiploid genome of B. napus. Some homoeologous regions were identified between the A and the C genomes. This map could be used to identify more markers, which would eventually be linked to genes controlling important agronomic characters in rapeseed. Furthermore, considering the good genome coverage we obtained, together with an observed homogenous distribution of the loci across the genome, this map is a powerful tool to be used in marker-assisted breeding.

Supplementary material

122_2005_80_MOESM1_ESM.pdf (97 kb)
Supplementary material

References

  1. Budin K, Just J, Thareau V, Belcram H, Moreau S, Laumonier M, Lecharny A, Aubourg S, Caboche M, Chalhoub B (2004) Physical mapping using new comparative genomic-based methods: functional physical markers for BAC-based happy mapping and contiguing of BAC clones in Brassica napus. Plant & Animal Genomes XIIth Conference, January 10–14, San Diego, CAGoogle Scholar
  2. Cheung WY, Champagne G, Hubert N, Landry BS (1997) Comparison of the genetic maps of Brassica napus and Brassica oleracea. Theor Appl Genet 94:569–582CrossRefGoogle Scholar
  3. Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA minipreparation: version II. Plant Mol Biol Reporter 1(4):19–21Google Scholar
  4. Ferreira ME, Satagopan J, Yandell BS, Williams PH, Osborn TC (1994) RFLP mapping of Brassica napus using doubled-haploid lines. Theor Appl Genet 89:615–621CrossRefGoogle Scholar
  5. Foisset N, Delourme R (1996) Segregation distorsion in androgenic plants. In: Mohan Jain S, Sopory S, Veilleux R (eds) In vitro haploid production in higher plants. Kluwer, Dordrecht, pp 189–201Google Scholar
  6. Foisset N, Delourme R, Barret P, Hubert N, Landry BS, Renard M (1996) Molecular mapping analysis in Brassica napus using isozyme, RAPD and RFLP markers on a doubled-haploid progeny. Theor Appl Genet 93:1017–1025CrossRefGoogle Scholar
  7. Landry BS, Hubert N, Etoh T, Harada J, Lincoln SE (1991) A genetic map for Brassica napus based on restriction fragment length polymorphisms detected with expressed DNA sequences. Genome 34:543–552Google Scholar
  8. Lombard V, Delourme R (2001) A consensus linkage map for rapeseed (Brassica napus L): construction and integration of three individual maps from DH populations. Theor Appl Genet 103:491–507CrossRefGoogle Scholar
  9. Lowe AJ, Moule C, Trick M, Edwards KJ (2004) Efficient large-scale development of microsatellites for marker and mapping applications in Brassica crop species. Theor Appl Genet 108:1103–1112CrossRefPubMedGoogle Scholar
  10. Osborn TC, Kole C, Parkin IA, Sharpe AG, Kuiper M, Lydiate DJ, Trick M (1997) Comparison of flowering time genes in Brassica rapa, B. napus and Arabidopsis thaliana. Genetics 146:1123–1129PubMedGoogle Scholar
  11. Parkin IAP, Lydiate D (1997) Conserved patterns of chromosome pairing and recombination in Brassica napus crosses. Genome 40:496–504CrossRefPubMedGoogle Scholar
  12. Parkin IAP, Sharpe AG, Keith DJ, Lydiate DJ (1995) Identification of the A and C genomes of amphidiploid Brassica napus (oilseed rape). Genome 38:1122–1131PubMedGoogle Scholar
  13. Parkin IAP, Sharpe AG, Lydiate DJ (2003) Patterns of genome duplication within the Brassica napus genome. Genome 46:291–303CrossRefPubMedGoogle Scholar
  14. Plieske J, Struss D (2001) Microsatellite markers for genome analysis in Brassica. I. Development in Brassica napus and abundance in Brassicaceae species. Theor Appl Genet 102:689–694CrossRefGoogle Scholar
  15. Saal B, Plieske J, Hu J, Quiros CF and Struss D (2001) Microsatellite markers for genome analysis in Brassica. II. Assignment of rapeseed microsatellites to the A and C genomes and genetic mapping in Brassica oleracea L. Theor Appl Genet 102:695–699CrossRefGoogle Scholar
  16. Schiex T, Gaspin C (1997) CARTHAGENE: constructing and joining maximum-likelihood genetic maps. In: Proceedings of the fifth international conference of intelligent systems for molecular biology, Porto Caras, Halkidiki, Greece, pp 258–267Google Scholar
  17. Sharpe AG, Parkin IAP, Keith DJ, Lydiate DJ (1995) Frequent non reciprocal translocations in the amphidiploid genome of oilseed rape (Brassica napus). Genome 38:1112–1121PubMedGoogle Scholar
  18. U N (1935) Genome analysis in Brassica with special reference to the experimental formation of B. napus and peculiar mode of fertilization. Jpn J Bot 7:389–452Google Scholar
  19. Udall JA, Quijada PA, Osborn TC (2005) Detection of chromosomal rearrangements derived from homoeologous recombination in four mapping populations of Brassica napus L. Genetics 169:967–979CrossRefPubMedGoogle Scholar
  20. Uzunova MI, Ecke W (1999) Abundance, polymorphism and genetic mapping of microsatellites in oilseed rape (Brassica napus L). Plant Breed 118:323–326CrossRefGoogle Scholar
  21. Uzunova M, Ecke W, Weissleder K, Robbelen G (1995) Mapping the genome of rapeseed (Brassica napus L.) I. Construction of an RFLP linkage map and localization of QTLs for seed glucosinolate content. Theor Appl Genet 90:194–204CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • J. Piquemal
    • 1
  • E. Cinquin
    • 1
  • F. Couton
    • 1
  • C. Rondeau
    • 1
  • E. Seignoret
    • 1
  • I. doucet
    • 1
  • D. Perret
    • 1
  • M.-J. Villeger
    • 1
  • P. Vincourt
    • 1
  • P. Blanchard
    • 1
  1. 1.Euralis Semences laboratoire de génétique moléculaireMondonvilleFrance

Personalised recommendations