Theoretical and Applied Genetics

, Volume 111, Issue 7, pp 1357–1364 | Cite as

A QTL located on chromosome 4A associated with dormancy in white- and red-grained wheats of diverse origin

  • D. Mares
  • K. Mrva
  • J. Cheong
  • K. Williams
  • B. Watson
  • E. Storlie
  • M. Sutherland
  • Y. Zou
Original Paper

Abstract

Improved resistance to preharvest sprouting in modern bread wheat (Triticum aestivum. L.) can be achieved via the introgression of grain dormancy and would reduce both the incidence and severity of damage due to unfavourable weather at harvest. The dormancy phenotype is strongly influenced by environmental factors making selection difficult and time consuming and this trait an obvious candidate for marker assisted selection. A highly significant Quantitative Trait Locus (QTL) associated with grain dormancy and located on chromosome 4A was identified in three bread wheat genotypes, two white- and one red-grained, of diverse origin. Flanking SSR markers on either side of the putative dormancy gene were identified and validated in an additional population involving one of the dormant genotypes. Genotypes containing the 4A QTL varied in dormancy phenotype from dormant to intermediate dormant. Based on a comparison between dormant red- and white-grained genotypes, together with a white-grained mutant derived from the red-grained genotype, it is concluded that the 4A QTL is a critical component of dormancy; associated with at least an intermediate dormancy on its own and a dormant phenotype when combined with the R gene in the red-grained genotype and as yet unidentified gene(s) in the white-grained genotypes. These additional genes appeared to be different in AUS1408 and SW95-50213.

Notes

Acknowledgements

Financial support from the Grains Research and Development Corporation and the Australian Centre for International Agricultural Research is gratefully acknowledged.

References

  1. Anderson JA, Sorrells ME, Tanksley SD (1993) RFLP analysis of genomic regions associated with resistance to preharvest sprouting in wheat. Crop Sci 33:453–459CrossRefGoogle Scholar
  2. Flintham JE, Gale MD (1996) Dormancy gene maps in homologous cereal genomes. In: Noda K, Mares DJ (eds) Preharvest Sprouting in Cereals 1995. Centre for Academic Societies, Osaka, Japan, pp 143–149Google Scholar
  3. Flintham JE, Adlam R, Gale MD (1999) Seedcoat and embryo dormancy in wheat. In: Weipert D (eds) Eighth International Symposium on Preharvest Sprouting in Cereals 1998. Association of Cereal Research, Federal Centre for Cereal Potato, and Lipid Research, Detmold, Germany, pp 67–76Google Scholar
  4. Flintham J, Adlam R, Bassoi M, Holdsworth M, Gale M (2002) Mapping genes for resistance to sprouting damage in wheat. Euphytica 126:39–45CrossRefGoogle Scholar
  5. Johnson JC, Clarke BC, Bhave M (2001) Isolation and characterization of cDNSs encoding protein disulphide Isomerases and Cyclophilins in wheat. J Cereal Sci 34:159–171CrossRefGoogle Scholar
  6. Kammholtz SJ, Campbell AW, Sutherland MW, Hollamby GJ, Martin PJ, Eastwood RF, Barclay I, Wilson RE, Brennan PS, Sheppard J (2001) Establishment and characterisation of wheat genetic mapping populations. Aust J Agric Res 52:1079–1088CrossRefGoogle Scholar
  7. Kato K, Nakamura W, Tabiki T, Miura H, Sawada S (2001) Detection of loci controlling seed dormancy in group 4 chromosomes of wheat and comparative mapping with rice and barley genomes. Theor Appl Genet 102:980–985CrossRefGoogle Scholar
  8. Kawakami N, Miyake Y, Noda K (1997) ABA insensitivity and low ABA levels during seed development of non-dormant wheat mutants. J Expt Bot 48:1415–1421CrossRefGoogle Scholar
  9. McIntosh RA, Hart GE, Devos KM, Gale MD (1995) Catalogue of gene symbols for wheat. 1995 supplement. Ann Wheat Newslett 41:330–335Google Scholar
  10. Manly KF, Cudmore Jr RH, Meer JM (2001) Map Manager QTX, cross-platform software for genetic mapping. Mammal Genome 12:930–932CrossRefGoogle Scholar
  11. Mares DJ (1983) Preservation of dormancy in freshly harvested wheat grain. Aust J of Agric Res 34:33–38CrossRefGoogle Scholar
  12. Mares DJ (1984) Temperature dependence of germinability of wheat grain (Triticum aestivum L.) grain in relation to preharvest sprouting. Aust J of Agric Res 35:115–128CrossRefGoogle Scholar
  13. Mares DJ (1987) Pre-harvest sprouting tolerance in white-grained wheat. In: Mares SJ (ed) Fourth international symposium on preharvest sprouting in cereals, Westview Press, Boulder pp 64–74Google Scholar
  14. Mares DJ (1989) In: Derera NF (ed) Preharvest sprouting damage and sprouting tolerance assay methods and instrumentation (Preharvest Field Sprouting in Cereals). CRC, Boca Raton, pp 130–166Google Scholar
  15. Mares DJ (1993) Genetic studies of sprouting tolerance in red and white wheats. In: Walker-Simmons K, Reid JL (eds) Preharvest sprouting in Cereals 1992. AACC, St Paul, MN, USA, pp 21–9Google Scholar
  16. Mares DJ (1996) Dormancy in white wheat : mechanism and location of genes. In: Noda K, Mares DJ (eds) Preharvest Sprouting in Cereals 1995’. Centre for Academic Societies, Osaka, Japan, pp 179–184Google Scholar
  17. Mares DJ (1999) The seed coat and dormancy in wheat grains. In: Weiprt D (ed) Eighth International Symposium on Preharvest Sprouting in Cereals 1998’. Association of Cereal Research Federal Centre for Cereal Potato and Lipid Research. Detmold, Germany, pp 77–81Google Scholar
  18. Mares DJ, Mrva K (2001) Mapping quantitative trait loci associated with variation in dormancy in Australian wheat. Aust J of Agric Res 52:1257–1266CrossRefGoogle Scholar
  19. Mori M, Uchino N, Chono M, Kato K, Miura H (2005) Mapping QTLs for grain dormancy on wheat chromosome 3A and the group 4 chromosomes, and their combined effect. Theor Appl Genet 110:1315–1323PubMedCrossRefGoogle Scholar
  20. Morris CF, Moffat JM, Sears RG, Paulsen GM (1989) Seed dormancy and responses of caryopses, embryos and calli to abscisic acid in wheat. Plant Physiol 90:643–647PubMedGoogle Scholar
  21. Noda K, Matsuura T, Maekawa M, Taketa S (2002) Chromosomes responsible for sensitivity of embryo to abscisic acid and dormancy in wheat. Euphytica 123:203–209CrossRefGoogle Scholar
  22. Paterson AH, Sorrells ME (1990) Inheritance of grain dormancy in white-kernelled wheat. Crop Sci 30:25–30CrossRefGoogle Scholar
  23. Ramsay L, Macaulay M, Ivanissivich S degli, MacLean K, Cardle L, Fuller J, Edwards K, Tuvesson S, Morgante M, Massari A, Maesti E, Marmiroli N, Sjakste T, Ganal M, Powell W, Waugh R (2000) A simple sequence repeat-based linkage map of barley. Theor Appl Genet 156:1997–2005Google Scholar
  24. Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier M-H, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023PubMedGoogle Scholar
  25. Rogowsky PM, Guidet FLY, Langridge P, Shepherd KW, Koebner RMD (1991) Isolation and characterization of wheat-rye recombinants involving chromosome arm 1DS of wheat. Theor Appl Genet 82:537–544CrossRefGoogle Scholar
  26. Trethowan RM (1995) Evaluation and selection of bread wheat (Triticum aestivum L.) for preharvest sprouting tolerance. Aust J Agric Res 46:463–474CrossRefGoogle Scholar
  27. Walker-Simmonds MK (1987) ABA levels and sensitivity in developing embryos of sprouting resistant and susceptible cultivars. Plant Physiol 84:61–66CrossRefGoogle Scholar
  28. Walker-Simmons MK (1988) Enhancement of ABA responsiveness in wheat embryos at higher temperature. Plant Cell Environ 11:769–775CrossRefGoogle Scholar
  29. Warner RL, Kurdna DA, Spaeth SC, Jones SS (2000) Dormancy in white-grained mutants of Chinese Spring wheat (Triticum aestivum L.). Seed Sci Res 10:51–60Google Scholar
  30. Williams KJ, Taylor SP, Bogacki P, Pallotta M, Bariana HS, Wallwork H (2002) Mapping of the root lesion nematode (Pratylenchus neglectus) resistance gene Rlnn1 in wheat. Theor Appl Genet 104:874–879CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • D. Mares
    • 1
  • K. Mrva
    • 1
  • J. Cheong
    • 2
  • K. Williams
    • 2
  • B. Watson
    • 3
  • E. Storlie
    • 3
  • M. Sutherland
    • 3
  • Y. Zou
    • 4
  1. 1.School of Agriculture and WineUniversity of AdelaideGlen OsmondAustralia
  2. 2.SARDIAdelaideAustralia
  3. 3.Faculty of SciencesUniversity of Southern QueenslandToowoombaAustralia
  4. 4.Sichuan Academy of Agricultural ScienceChengdu CityChina

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