Theoretical and Applied Genetics

, Volume 110, Issue 5, pp 895–905

Distinct post-transcriptional modifications result into seven alternative transcripts of the CC–NBS–LRR gene JA1tr of Phaseolus vulgaris

  • Elodie Ferrier-Cana
  • Catherine Macadré
  • Mireille Sévignac
  • Perrine David
  • Thierry Langin
  • Valérie Geffroy
Original Paper

DOI: 10.1007/s00122-004-1908-1

Cite this article as:
Ferrier-Cana, E., Macadré, C., Sévignac, M. et al. Theor Appl Genet (2005) 110: 895. doi:10.1007/s00122-004-1908-1

Abstract

The generation of splice variants has been reported for various plant resistance (R) genes, suggesting that these variants play an important role in disease resistance. Most of the time these R genes belong to the Toll and mammalian IL-1 receptor–nucleotide-binding site–leucine-rich repeat (TIR–NBS–LRR) class of R genes. In Phaseolus vulgaris, a resistance gene cluster (referred to as the B4 R-gene cluster) has been identified at the end of linkage group B4. At this complex resistance cluster, three R specificities (Co-9, Co-y and Co-z) and two R QTLs effective against the fungal pathogen Colletotrichum lindemuthianum, the causal agent of anthracnose, have been identified. At the molecular level, four resistance gene candidates encoding putative full-length, coiled-coil (CC)–NBS–LRR R-like proteins, with LRR numbers ranging from 18 to 20, have been previously characterized. In the present study, seven cDNA corresponding to truncated R-like transcripts, belonging to the CC–NBS–LRR class of plant disease R genes, have been identified. These seven transcripts correspond to a single gene named JA1tr, which encodes, at most, only five LRRs. The seven JA1tr transcript variants result from distinct post-transcriptional modifications of JA1tr, corresponding to alternative splicing events of two introns, exon skipping and multiple ‘aberrant splicing’ events in the open reading frame (ORF). JA1tr was mapped at the B4 R-gene cluster identified in common bean. These post-transcriptional modifications of the single gene JA1tr could constitute an efficient source of diversity. The present results provide one of the few reports of transcript variants with truncated ORFs resulting from a CC–NBS–LRR gene.

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Elodie Ferrier-Cana
    • 1
  • Catherine Macadré
    • 1
  • Mireille Sévignac
    • 1
  • Perrine David
    • 1
  • Thierry Langin
    • 1
  • Valérie Geffroy
    • 1
  1. 1.Institut de Biotechnologie des Plantes, UMR-CNRS 8618Université Paris-SudOrsayFrance

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