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Theoretical and Applied Genetics

, Volume 110, Issue 4, pp 730–741 | Cite as

Two classes of 5S rDNA unit arrays of the silver fir, Abies alba Mill.: structure, localization and evolution

  • Višnja Besendorfer
  • Iva Krajačić-Sokol
  • Srećko Jelenić
  • Jasna Puizina
  • Jelena Mlinarec
  • Tonka Sviben
  • Dražena Papeš
Original Paper

Abstract

The structure and organization of the 5S ribosomal DNA units of the silver fir, Abies alba Mill., as well as their position in the chromosome complement were investigated. PCR amplification of the gene and nontranscribed spacer region, sequence analysis and Southern hybridization, using a homologous probe, detected DNA sequences of approximately 550 bp and 700 bp. Sequence analysis of the spacers revealed that the difference in length between the sequences occurred in the middle spacer region as a result of the amplification of a 75-bp sequence of the short unit class, which is organized in four 54- to 68-bp tandem repeats in the long spacer unit. The 5S rDNA transcribed region is 120 bp long and shows high sequence similarity with other gymnosperm species. The comparative analysis of 5′ and 3′ flanking sequences of 5S rRNA genes of silver fir and other gymnosperms indicates that A. alba spacer units have the same rate of evolution and are more closely related to Larix and Pseudotsuga than to Pinus and Picea. Southern hybridization and fluorescence in situ hybridization of metaphase chromosomes of A. alba suggest that the short and long spacer units are organized as separate tandem arrays at two chromosomal loci on chromosomes V and XI.

Keywords

Concerted Evolution Space Class Unit Class High Similarity Score Phenetic Analysis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

The authors thank M. Gradečki from the Forestry Research Institute ‘Jasrebarsko’ for preparing the plant material used in the cytogenetic study and K. Vlahoviček for sequence analysis and suggestions in the interpretation of the phylogeny results. This work was supported by research grant no. 119112 from the Ministry of Science, Education and Sport of the Republic of Croatia. Jasna Puizina acknowledges the support of the Gregor Mendel Institute, Vienna, Austria.

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Višnja Besendorfer
    • 1
  • Iva Krajačić-Sokol
    • 1
  • Srećko Jelenić
    • 1
  • Jasna Puizina
    • 2
    • 3
  • Jelena Mlinarec
    • 1
  • Tonka Sviben
    • 1
  • Dražena Papeš
    • 1
  1. 1.Department of Molecular Biology, Faculty of ScienceUniversity of ZagrebZagrebCroatia
  2. 2.Department of Biology, Faculty of Natural Sciences and MathematicsUniversity of SplitSplitCroatia
  3. 3.Gregor Mendel Institute of Molecular Plant BiologyAustrian Academy of ScienceViennaAustria

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