Simple-sequence repeat markers used in merging linkage maps of melon (Cucumis melo L.)
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A set of 118 simple sequence repeat (SSR) markers has been developed in melon from two different sources: genomic libraries (gSSR) and expressed sequence-tag (EST) databases (EST-SSR). Forty-nine percent of the markers showed polymorphism between the ‘Piel de Sapo’ (PS) and PI161375 melon genotypes used as parents for the mapping populations. Similar polymorphism levels were found in gSSR (51.2%) and EST-SSR (45.5%). Two populations, F2 and a set of double haploid lines (DHLs), developed from the same parent genotypes were used for map construction. Twenty-three SSRs and 79 restriction fragment length polymorphisms (RFLPs), evenly distributed through the melon genome, were used to anchor the maps of both populations. Ten cucumber SSRs, 41 gSSRs, 16 EST-SSR, three single nucleotide polymorphism (SNP) markers, and the Nsv locus were added in the DHL population. The maps developed in the F2 and DHL populations were co-linear, with similar lengths, except in linkage groups G1, G9, and G10. There was segregation distortion in a higher proportion of markers in the DHL population compared with the F2, probably caused by selection during the construction of DHLs through in vitro culture. After map merging, a composite genetic map was obtained including 327 transferable markers: 226 RFLPs, 97 SSRs, three SNPs, and the Nsv locus. The map length is 1,021 cM, distributed in 12 linkage groups, and map density is 3.11 cM/marker. SSR markers alone cover nearly 80% of the map length. This map is proposed as a basis for a framework melon map to be merged with other maps and as an anchor point for map comparison between species of the Cucurbitaceae family.
KeywordsLinkage Group Melon Simple Sequence Repeat Marker Segregation Distortion Double Haploid Line
The authors thank V. Alfaro and N. Galofré for technical assistance. This work was funded in part by grants for projects AGL2000-0360 and AGL2003-09175-C02-01 from The Spanish Ministry of Science and Technology and by the Semillas Fitó-Institut de Recerca i Tecnologia Agroalimentàries (IRTA) joint programme. A.J.M. was supported by a contract from Instituto Nacional de Investigaciones Agrarias (INIA). M.J.G. was supported by a fellowship from Institut de Recerca I Tecnologia Agroalimentàries (IRTA). The experiments presented here comply with current Spanish law.
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