Theoretical and Applied Genetics

, Volume 110, Issue 1, pp 126–135

Genetic diversity associated with variation in silage corn digestibility for three O-methyltransferase genes involved in lignin biosynthesis

  • C. Guillet-Claude
  • C. Birolleau-Touchard
  • D. Manicacci
  • M. Fourmann
  • S. Barraud
  • V. Carret
  • J. P. Martinant
  • Y. Barrière
Original Paper

DOI: 10.1007/s00122-004-1808-4

Cite this article as:
Guillet-Claude, C., Birolleau-Touchard, C., Manicacci, D. et al. Theor Appl Genet (2004) 110: 126. doi:10.1007/s00122-004-1808-4

Abstract

Polymorphisms within three candidate genes for lignin biosynthesis were investigated to identify alleles useful for the improvement of maize digestibility. The allelic diversity of two caffeoyl-CoA 3-O-methyltransferase genes, CCoAOMT2 and CCoAOMT1, as well as that of the aldehyde O-methyltransferase gene, AldOMT, was evaluated for 34 maize lines chosen for their varying degrees of cell wall digestibility. Frequency of nucleotide changes averaged one SNP every 35 bp. Ninety-one indels were identified in non-coding regions and only four in coding regions. Numerous distinct and highly diverse haplotypes were identified at each locus. Numerous sites were in linkage disequilibrium that declined rapidly within a few hundred bases. For F4, an early flint French line with high cell wall digestibility, the CCoAOMT2 first exon presented many non-synonymous polymorphisms. Notably we found an 18-bp indel, which resembled a microsatellite and was associated with cell wall digestibility variation. Additionally, the CCoAOMT2 gene co-localized with a QTL for cell wall digestibility and lignin content. Together, these results suggest that genetic diversity investigated on a broader genetic basis could contribute to the identification of favourable alleles to be used in the molecular breeding of elite maize germplasm.

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • C. Guillet-Claude
    • 1
    • 4
  • C. Birolleau-Touchard
    • 1
  • D. Manicacci
    • 2
  • M. Fourmann
    • 2
  • S. Barraud
    • 2
  • V. Carret
    • 1
  • J. P. Martinant
    • 3
  • Y. Barrière
    • 1
  1. 1.Unité de Génétique et d’Amélioration des Plantes FourragèresINRALusignanFrance
  2. 2.Station de Génétique VégétaleUMR INRA-UPS-CNRS-INAPGGif sur YvetteFrance
  3. 3.Biogemma S.A.S.Campus Universitaire des CézeauxAubiereFrance
  4. 4.Centre de recherche en biologie forestièreUniversité LavalQuébecCanada

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