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Theoretical and Applied Genetics

, Volume 110, Issue 1, pp 48–57 | Cite as

Identification of quantitative trait loci controlling resistance to maize chlorotic dwarf virus

  • Mark W. Jones
  • Margaret G. Redinbaugh
  • Robert J. Anderson
  • R. Louie
Original Paper

Abstract

Ineffective screening methods and low levels of disease resistance have hampered genetic analysis of maize (Zea mays L.) resistance to disease caused by maize chlorotic dwarf virus (MCDV). Progeny from a cross between the highly resistant maize inbred line Oh1VI and the susceptible inbred line Va35 were evaluated for MCDV symptoms after multiple virus inoculations, using the viral vector Graminella nigrifrons. Symptom severity scores from three rating dates were used to calculate area under the disease progress curve (AUDPC) scores for vein banding, leaf twist and tear, and whorl chlorosis. AUDPC scores for the F2 population indicated that MCDV resistance was quantitatively inherited. Genotypic and phenotypic analyses of 314 F2 individuals were compared using composite interval mapping (CIM) and analysis of variance. CIM identified two major quantitative trait loci (QTL) on chromosomes 3 and 10 and two minor QTL on chromosomes 4 and 6. Resistance was additive, with alleles from Oh1VI at the loci on chromosomes 3 and 10 contributing equally to resistance.

Keywords

Quantitative Trait Locus Simple Sequence Repeat Marker Composite Interval Mapping Major Quantitative Trait Locus Wheat Streak Mosaic Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

We thank Jean Vacha, Pam Jacobs, and Jane Todd for expert technical assistance. Mention of a trademark, proprietary product, trade names or commercial products in this article is solely for the purpose of providing scientific information; it does not constitute a guarantee, warranty, recommendation, or endorsement by the USDA and does not imply approval to the exclusion of other products that also may be suitable.

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Mark W. Jones
    • 1
  • Margaret G. Redinbaugh
    • 1
    • 2
  • Robert J. Anderson
    • 1
  • R. Louie
    • 1
    • 2
  1. 1.USDA, ARS Corn and Soybean Research, Ohio Agriculture Research and Development Center (OARDC)The Ohio State UniversityWoosterUSA
  2. 2.Department of Plant Pathology, Ohio Agriculture Research and Development Center (OARDC)The Ohio State UniversityWoosterUSA

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