A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.)
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A microsatellite consensus map was constructed by joining four independent genetic maps of bread wheat. Three of the maps were F1-derived, doubled-haploid line populations and the fourth population was ‘Synthetic’ × ‘Opata’, an F6-derived, recombinant-inbred line population. Microsatellite markers from different research groups including the Wheat Microsatellite Consortium, GWM, GDM, CFA, CFD, and BARC were used in the mapping. A sufficient number of common loci between genetic maps, ranging from 52 to 232 loci, were mapped on different populations to facilitate joining the maps. Four genetic maps were developed using MapMaker V3.0 and JoinMap V3.0. The software CMap, a comparative map viewer, was used to align the four maps and identify potential errors based on consensus. JoinMap V3.0 was used to calculate marker order and recombination distances based on the consensus of the four maps. A total of 1,235 microsatellite loci were mapped, covering 2,569 cM, giving an average interval distance of 2.2 cM. This consensus map represents the highest-density public microsatellite map of wheat and is accompanied by an allele database showing the parent allele sizes for every marker mapped. This enables users to predict allele sizes in new breeding populations and develop molecular breeding and genomics strategies.
KeywordsChinese Spring Marker Order Segregation Data Allele Pair Paralogous Locus
The authors wish to thank all the members of the WMC for assisting with marker development, Dr. P. Sourdille (INRA) for providing the CFA and CFD sequences, and Dr. P. Cregan for providing the BARC sequences. We are also grateful to Drs. G. Fedak, F. Townley-Smith, and G. Humphreys for providing the mapping populations. Many people worked to provide segregation data including Janice Cuthbert, Curt McCartney, Mira Popovic, Zlatko Popovic, Monika Eng, Yanfen Zheng, Leslie Bezte, Brent McCallum, Gavin Humphreys, Odean Lukow, Nancy Ames (AAFC), and Laurie Bertrand (Agrogene). This is contribution no. 1882 from the Agriculture and Agri-Food Canada Cereal Research Centre.
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