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Theoretical and Applied Genetics

, Volume 109, Issue 5, pp 1070–1076 | Cite as

Genomic constitution and variation in five partial amphiploids of wheat–Thinopyrum intermedium as revealed by GISH, multicolor GISH and seed storage protein analysis

  • Fangpu HanEmail author
  • Bao Liu
  • George FedakEmail author
  • Zhaohui Liu
Original Paper

Abstract

Genomic in situ hybridization (GISH) and multicolor GISH (mcGISH) methodology were used to establish the cytogenetic constitution of five partial amphiploid lines obtained from wheat × Thinopyrum intermedium hybridizations. Line Zhong 1, 2n=52, contained 14 chromosomes from each of the wheat genomes plus ten Th. intermedium chromosomes, with one pair of A-genome chromosomes having a Th. intermedium chromosomal segment translocated to the short arm. Line Zhong 2, 2n=54, had intact ABD wheat genome chromosomes plus 12 Th. intermedium chromosomes. The multicolor GISH results, using different fluorochrome labeled Th. intermedium and the various diploid wheat genomic DNAs as probes, indicated that both Zhong 1 and Zhong 2 contained one pair of Th. intermedium chromosomes with a significant homology to the wheat D genome. High-molecular-weight (HMW) glutenin and gliadin analysis revealed that Zhong 1 and Zhong 2 had identical banding patterns that contained all of the wheat bands and a specific HMW band from Th. intermedium. Zhong 1 and Zhong 2 had good HMW subunits for wheat breeding. Zhong 3 and Zhong 5, both 2n=56, possessed no gross chromosomal aberrations or translocations that were detectable at the GISH level. Zhong 4 also had a chromosome number of 2n=56 and contained the complete wheat ABD-genome chromosomes plus 14 Th. intermedium chromosomes, with one pair of Th. intermedium chromosomes being markedly smaller. Multicolor GISH results indicated that Zhong 4 also contained two pairs of reciprocally translocated chromosomes involving the A and D genomes. Zhong 3, Zhong 4 and Zhong 5 contained a specific gliadin band from Th. intermedium. Based on the above data, it was concluded that inter-genomic transfer of chromosomal segments and/or sequence introgression had occurred in these newly synthesized partial amphiploids despite their diploid-like meiotic behavior and disomic inheritance.

Keywords

Wheat Chromosome Genomic Change Alien Chromosome Genomic Constitution Wheat Streak Mosaic Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

We are grateful to Mr. Zhang Yanbin and Ms. Gao Zhi for technical assistance in the seed storage protein analysis and for maintaining the plants over the years. This study was supported in part by the National Science Fund for Distinguished Young Scholars of China (no. 30225003).

References

  1. Banks PM, Xu SJ, Wang R-C, Larkin PJ (1993) Varying chromosome composition of 56-chromosome wheat-Thinopyrum intermedium partial amphiploids. Genome 36:207–215Google Scholar
  2. Cauderon Y, Saigne B, Dauge M (1973) The resistance to wheat rusts of Agropyron intermedium and its use in wheat improvement. In: Sears ER, Sears LMS (eds) Proc 4th Int Wheat Genet Symp. University of Missouri, Columbia, Mo., pp 401–407Google Scholar
  3. Chen Q, Conner RL, Li HJ, Sun SC, Ahmad F, Laroche A, Graf RJ (2003) Molecular cytogenetic discrimination and reaction to wheat streak mosaic virus and the wheat curl mite in Zhong series of wheat-Thinopyrum intermedium partial amphiploids. Genome 46:135–145CrossRefPubMedGoogle Scholar
  4. Chi SY, Yu SS, Chang YH, Yu KH, Song FY (1979) Studies on wheat breeding by distant hybridization between wheat and Agropyron glaucum. Sci Agric Sin 2:1–11Google Scholar
  5. Dvorak J, Dubcovsky J (1995) Recombination between homoeologous chromosomes in wheat in the absence of the Phl locus. Classical and molecular cytogenetic analysis. In: Raupp WJ, Gill BS (eds) Proc USA-Jpn Symp. Kansas Agric Exp Sta Rep, Manhattan, Kan., pp 64–75Google Scholar
  6. Fedak G (1999) Molecular aids for integration of alien chromatin through wide crosses. Genome 42:584–591Google Scholar
  7. Fedak G, Han FP (2004) Characterization of derivatives from wheat-Thinopyrum wide crosses. Cytogenet Genome Res (in press)Google Scholar
  8. Fedak G, Chen Q, Conner RL, Laroche A, Petroski R, Armstrong KC (2000) Characterization of wheat-Thinopyrum partial amphiploids by meiotic analysis and genomic in situ hybridization. Genome 43:712–719CrossRefPubMedGoogle Scholar
  9. Feldman M, Levy AA (2003) Acceleration of genome evolution by allopolyploidy: wheat as a model. In: Pogna NE et al. (eds) Proc 10th Int Wheat Genet Symp. Instituto Sperimentale Per La Cerealcultura, Paestum, pp 11–16Google Scholar
  10. Feldman M, Liu B, Segal G, Abbo S, Levy AA, Vega JM (1997) Rapid elimination of low-copy DNA sequences in polyploid wheat: a possible mechanism for differentiation of homoeologous chromosomes. Genetics 147:1381–1387PubMedGoogle Scholar
  11. Friebe B, Mukai Y, Gill BS, Cauderon Y (1992) C-banding and in situ hybridization analyses of Agropyron intermedium, a partial wheat × Ag. intermedium amphiploid, and six derived chromosome addition lines. Theor Appl Genet 84:899–905Google Scholar
  12. Galili G, Feldman M (1984) Intergenomic suppression of endosperm protein genes in common wheat. Can J Genet Cytol 26:651–656Google Scholar
  13. Gao Z, Han FP, He MY, Ma YZ, Xin ZY (1999) Characterization of genome and chromosomes in octoploid wheat-wheatgrass amphiploid Zhong 2 using fluorescence in situ hybridization and chromosome pairing analysis. Acta Bot Sin 41:25–28Google Scholar
  14. Han FP (1994) Study of genome constitution of Elytrigia intermedia and octoploid Trititrigia. Hereditas 16:31–34Google Scholar
  15. Han FP, Li JL (1995) Partial amphiploids from Triticum durum × Elytrigia intermedia and T. durum × tetraploid Elytrigia elongata. Wheat Inf Serv 80:32–36Google Scholar
  16. Han FP, He MY, Hao S, Ma YZ, Xin ZY (1998a) Variation of wheatgrass chromosomes in wheat-wheatgrass disomic addition line TAI-14 revealed by fluorescence in situ hybridization. Acta Bot Sin 40:33–36Google Scholar
  17. Han FP, Zhang XQ, Bu XL, He MY, Hao S, Ma YZ, Xin ZY (1998b) Variation of wheatgrass chromosomes in wheat-wheatgrass alien addition line “TAI-27” revealed by fluorescence in situ hybridization (FISH). Sci China Ser C 41:366–371Google Scholar
  18. Han FP, Fedak G, Benabdelmouna A, Armstrong K, Ouellet T (2003) Characterization of six wheat × Thinopyrum intermedium derivatives by GISH, RFLP and multicolor GISH. Genome 46:490–495CrossRefPubMedGoogle Scholar
  19. He MY, Xu ZY, Zou MQ, Zhang H, Piao ZS, Hao S (1988) The establishment of two sets of alien addition lines of wheat-wheatgrass. Sci China Ser B 32:695–705Google Scholar
  20. He MY, Chen DW, Zhang XQ, Hao S (1993) Transfer of useful genes from wheatgrass into common wheat by chromosome engineering technique. In: Li ZS, Xin ZY (eds) Proc 8th Int Wheat Genet Symp. China Agricultural Scientech Press, Beijing, pp 165–168Google Scholar
  21. Kashkush K, Feldman M, Levy AA (2002) Gene loss, silencing and activation in a newly synthesized wheat allotetraploid. Genetics 160:1651–1659Google Scholar
  22. Kashkush K, Feldman M, Levy AA (2003) Transcriptional activation of retrotransposons alters the expression of adjacent genes in wheat. Nat Genet 33:102–106CrossRefPubMedGoogle Scholar
  23. Kidwell KK, Osborn TC (1992) Simple plant DNA isolation procedures, In: Beckman JS, Osborn TC (eds) Plant genomes: methods for genetic and physical mapping. Kluwer, Dordrecht, pp 1–13Google Scholar
  24. Larkin PJ, Banks PM, Lagudah ES, Apple R, Chen X, Xin Z Y, Ohm HW, McIntosh RA (1995) Disomic Thinopyrum intermedium addition lines in wheat with barley yellow dwarf virus resistance and with rust resistances. Genome 38:385–394Google Scholar
  25. Levy AA, Feldman M (2002) The impact of polyploidy on grass genome evolution. Plant Physiol 130:1587–1593CrossRefPubMedGoogle Scholar
  26. Liu B, Wendel JF (2002) Non-Mendelian phenomena in allopolyploid genome evolution. Curr Genomics 3:489–506Google Scholar
  27. Liu B, He MY, Hao S (1999) Study on genomic changes in partial amphiploids of common wheat-wheatgrass. Acta Bot Sin 41:591–596Google Scholar
  28. Matzke MA, Scheid OM, Matzke AJM (1999) Rapid structural and epigenetic changes in polyploid and aneuploid genomes. Bioessays 21:761–767CrossRefPubMedGoogle Scholar
  29. Payne P, Lookhart GL, Forsyth SA (1988) The high molecular weight glutenin subunit composition of two closely related lines of wheat that have contrasting breadmaking qualities. J Cereal Sci 8:285–288Google Scholar
  30. Riley R (1960) The diploidization of polyploid wheat. Heredity 15:407–429Google Scholar
  31. Schulz-Schaeffer J, Haller S E (1988) Alien chromosome addition in durum wheat. II. Advanced progeny. Genome 30:303–306Google Scholar
  32. Sears ER (1976) Genetic control of chromosome pairing in wheat. Annu Rev Genet 10:31–51PubMedGoogle Scholar
  33. Sun SC (1981) The approach and methods of breeding new varieties and new species from Agrotriticum hybrids. Acta Agron Sin 7:51–58Google Scholar
  34. Wienhues A (1966) Transfer of rust resistance of Agropyron to wheat by addition, substitution and translocation. Hereditas 2[Suppl]:328–341Google Scholar
  35. Zhang YB, Qi SY, Xiao ZM, Xin WL, Gao Z, Han FP (1997a) Study of a noncontinuous formic acid-PAGE method of the wheat. J Harbin Normal Univ 13:70–73Google Scholar
  36. Zhang YB, Qi SY, Xiao ZM, Xin WL, Gao Z, Han FP (1997b) A practical SDS-PAGE method of HMW subunits of wheat for quality breeding of wheat in China. J Harbin Normal Univ 13:60–63Google Scholar

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  1. 1.Eastern Cereal and Oilseed Research CentreAgriculture and Agri-Food CanadaOttawaCanada
  2. 2.Division of Biological SciencesUniversity of MissouriColumbiaUSA
  3. 3.School of Life SciencesNortheast Normal UniversityChangchunChina
  4. 4.Department of Plant PathologyNorth Darkata UniversityFargoUSA

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