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Theoretical and Applied Genetics

, Volume 109, Issue 5, pp 922–932 | Cite as

Pine genes regulated by the necrotrophic pathogen Fusarium circinatum

  • Alison M. Morse
  • C. Dana Nelson
  • Sarah F. Covert
  • Angela G. Holliday
  • Katherine E. Smith
  • John M. DavisEmail author
Original Paper

Abstract

A targeted genomics approach was used to construct a cDNA array of potential pathogen-regulated genes for investigating host–pathogen interactions in pine trees (Pinus species). This array, containing a nonredundant set of 311 cDNAs, was assembled by combining smaller sets of cDNAs generated by differential display or suppression subtraction hybridization using a variety of pathogen treatments and elicitors. The array was probed to identify host genes regulated by Fusarium circinatum, a necrotrophic fungus that incites pitch canker disease on pine stems. A set of 29 cDNAs were induced during the disease state. Notably, cDNAs on the array that were derived from experiments with fusiform rust, incited by Cronartium quercuum f. sp. fusiforme (a biotrophic fungus) were unregulated by Fusarium. The results imply distinct genetic responses in pine to diseases incited by necrotrophs and biotrophs. This cDNA collection expands the genomics toolkit for understanding interactions between conifers and their microbial associates in forest ecosystems.

Keywords

Chitinase Suppression Subtraction Hybridization Chitinase Gene Global Standardization Internal Reference Standardization 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

This is journal article number R-10259 of the Florida Agricultural Experiment Station. The work was supported by the USDA Forest Service-Southern Research Station (Agreement no. SRS 00-CA-11330126-016), USDA-CSREES IFAFS (Grant no. 2001-52100-11315), and the Consortium for Plant Biotechnology (Agreement no. OR 22072-104) (to J.D.) and Georgia’s Traditional Industries Program in Pulp and Paper (to S.C.). We gratefully acknowledge T. Korhnak for technical assistance on differential display, C. Dervinis for critical assistance with plant maintenance and suggestions on the manuscript, and Basma El-Yacoubi for helpful discussions.

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Alison M. Morse
    • 1
  • C. Dana Nelson
    • 2
  • Sarah F. Covert
    • 3
  • Angela G. Holliday
    • 3
  • Katherine E. Smith
    • 1
    • 2
  • John M. Davis
    • 1
    • 4
    Email author
  1. 1.School of Forest Resources and ConservationUniversity of FloridaGainesvilleUSA
  2. 2.USDA Forest ServiceSouthern Institute of Forest GeneticsSaucierUSA
  3. 3.Daniel B. Warnell School of Forest ResourcesUniversity of GeorgiaAthensUSA
  4. 4.Program in Plant Molecular and Cellular BiologyUniversity of FloridaGainesvilleUSA

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