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Theoretical and Applied Genetics

, Volume 109, Issue 1, pp 210–214 | Cite as

Localization of a novel recessive powdery mildew resistance gene from common wheat line RD30 in the terminal region of chromosome 7AL

  • Ch. Singrün
  • S. L. K. Hsam
  • F. J. Zeller
  • G. Wenzel
  • V. Mohler
Original Paper

Abstract

Segregation analysis of resistance to powdery mildew in a F2 progeny from the cross Chinese Spring (CS) × TA2682c revealed the inheritance of a dominant and a recessive powdery mildew resistance gene. Selfing of susceptible F2 individuals allowed the establishment of a mapping population segregating exclusively for the recessive resistance gene. The extracted resistant derivative showing full resistance to each of 11 wheat powdery mildew isolates was designated RD30. Amplified fragment length polymorphism (AFLP) analysis of bulked segregants from F3s showing the homozygous susceptible and resistant phenotypes revealed an AFLP marker that was associated with the recessive resistance gene in repulsion phase. Following the assignment of this AFLP marker to wheat chromosome 7A by means of CS nullitetrasomics, an inspection of simple sequence repeat (SSR) loci evenly spaced along chromosome 7A showed that the recessive resistance gene maps to the distal region of chromosome 7AL. On the basis of its close linkage to the Pm1 locus, as inferred from connecting partial genetic maps of 7AL of populations CS × TA2682c and CS × Virest (Pm1e), and its unique disease response pattern, the recessive resistance gene in RD30 was considered to be novel and tentatively designated mlRD30.

Keywords

Amplify Fragment Length Polymorphism Powdery Mildew Amplify Fragment Length Polymorphism Marker Chinese Spring Restriction Fragment Length Polymorphism Marker 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

The authors gratefully acknowledge Amalie Fiedler and Christina Hartmann for their excellent technical assistance and the anonymous referees for valuable suggestions.

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Ch. Singrün
    • 1
  • S. L. K. Hsam
    • 2
  • F. J. Zeller
    • 2
  • G. Wenzel
    • 1
  • V. Mohler
    • 1
  1. 1.Chair of Agronomy and Plant Breeding, Department of Plant Sciences, Center for Life and Food Sciences WeihenstephanTechnical University Munich FreisingGermany
  2. 2.Division of Plant Breeding and Applied Genetics, Department of Plant Sciences, Center for Life and Food Sciences WeihenstephanTechnical University MunichFreisingGermany

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