Theoretical and Applied Genetics

, Volume 109, Issue 1, pp 210–214 | Cite as

Localization of a novel recessive powdery mildew resistance gene from common wheat line RD30 in the terminal region of chromosome 7AL

  • Ch. Singrün
  • S. L. K. Hsam
  • F. J. Zeller
  • G. Wenzel
  • V. MohlerEmail author
Original Paper


Segregation analysis of resistance to powdery mildew in a F2 progeny from the cross Chinese Spring (CS) × TA2682c revealed the inheritance of a dominant and a recessive powdery mildew resistance gene. Selfing of susceptible F2 individuals allowed the establishment of a mapping population segregating exclusively for the recessive resistance gene. The extracted resistant derivative showing full resistance to each of 11 wheat powdery mildew isolates was designated RD30. Amplified fragment length polymorphism (AFLP) analysis of bulked segregants from F3s showing the homozygous susceptible and resistant phenotypes revealed an AFLP marker that was associated with the recessive resistance gene in repulsion phase. Following the assignment of this AFLP marker to wheat chromosome 7A by means of CS nullitetrasomics, an inspection of simple sequence repeat (SSR) loci evenly spaced along chromosome 7A showed that the recessive resistance gene maps to the distal region of chromosome 7AL. On the basis of its close linkage to the Pm1 locus, as inferred from connecting partial genetic maps of 7AL of populations CS × TA2682c and CS × Virest (Pm1e), and its unique disease response pattern, the recessive resistance gene in RD30 was considered to be novel and tentatively designated mlRD30.


Amplify Fragment Length Polymorphism Powdery Mildew Amplify Fragment Length Polymorphism Marker Chinese Spring Restriction Fragment Length Polymorphism Marker 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The authors gratefully acknowledge Amalie Fiedler and Christina Hartmann for their excellent technical assistance and the anonymous referees for valuable suggestions.


  1. Dyck PL, Kerber ER (1981) Aneuploid analysis of a gene for leaf rust resistance derived from the common wheat cultivar Terenzio. Can J Genet Cytol 23:405–409Google Scholar
  2. Felsenstein FG, Jaser B (2000) Effectiveness of qualitative powdery mildew resistance in wheat and barley and sensitivity of fungal cereal pathogens towards different compounds.
  3. Hsam SLK, Zeller FJ (1997) Evidence of allelism between genes Pm8 and Pm17 and chromosomal location of powdery mildew and leaf rust resistance genes in the common wheat cultivar Amigo. Plant Breed 116:110–122Google Scholar
  4. Hsam SLK, Zeller FJ (2002) Breeding for powdery mildew resistance in common wheat (T. aestivum L. em Thell.). In: Bélanger RR, Bushnell WR, Dik AJ, Carver TWL (eds) The powdery mildews: a comprehensive treatise, APS Press, St. Paul, Minn., pp 219–238Google Scholar
  5. Hsam SLK, Lapochkina IF, Zeller FJ (2003) Chromosomal location of genes for resistance to powdery mildew in common wheat (Triticum aestivum L. em Thell.). 8. Gene Pm32 in a wheat-Aegilops speltoides translocation line. Euphytica 133:367–370CrossRefGoogle Scholar
  6. Huang XQ, Zeller FJ, Hsam SLK, Wenzel G, Mohler V (2000a) Chromosomal location of AFLP markers in common wheat utilizing nulli-tetrasomic stocks. Genome 43:298–305PubMedGoogle Scholar
  7. Huang XQ, Zeller FJ, Hsam SLK, Wenzel G, Mohler V (2000b) Molecular mapping of wheat powdery mildew resistance gene Pm24 and marker validation for molecular breeding. Theor Appl Genet 101:407–414CrossRefGoogle Scholar
  8. Huang XQ, Wang LX, Xu MX, Röder MS (2003) Microsatellite mapping of the powdery mildew resistance gene Pm5e in common wheat (Triticum aestivum L.). Theor Appl Genet 106:858–865PubMedGoogle Scholar
  9. Knott DR (1968) The inheritance of resistance to stem rust races 56 and 15B-1L (Can.) in the wheat varieties Hope and H-44. Can J Genet Cytol 10:311–320Google Scholar
  10. Lebsock KL, Briggle LW (1974) Gene Pm5 for resistance to Erysiphe graminis f. sp. tritici in Hope wheat. Crop Sci 14:561–563Google Scholar
  11. Liu J, Liu D, Tao W, Li W, Wang S, Chen P, Cheng S, Gao D (2000) Molecular marker-facilitated pyramiding of different genes for powdery mildew resistance in wheat. Plant Breed 119:21–24CrossRefGoogle Scholar
  12. Lupton FGH, Macer RCF (1962) Inheritance of resistance to yellow rust (Puccinia glumarum Erikss. & Henn.) in seven varieties of wheat. Trans Br Mycol Soc 45:21–45Google Scholar
  13. McIntosh RA, Luig NH, Baker EP (1967) Genetic and cytogenetic studies of stem rust, leaf rust and powdery mildew resistances in Hope and related wheat cultivars. Aust J Biol Sci 20:1181–1192Google Scholar
  14. McIntosh RA, Hart GE, Devos KM, GaleMD, Rogers WJ (1998) Catalogue of gene symbols for wheat.
  15. McIntosh RA, Devos KM, Dubcovsky J, Morris CF, Rogers WJ (2003) Catalogue of gene symbols for wheat: 2003 supplement.
  16. Mester DI, Ronin YI, Hu Y, Peng J, Nevo E, Korol AB (2003) Efficient multipoint mapping: making use of dominant repulsion-phase markers. Theor Appl Genet 107:1102–1112CrossRefPubMedGoogle Scholar
  17. Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832PubMedGoogle Scholar
  18. Mohler V, Hsam SLK, Zeller FJ, Wenzel G (2001) An STS marker distinguishing the rye-derived powdery mildew resistance alleles at the Pm8/Pm17 locus of common wheat (T. aestivum L. em Thell.). Plant Breed 120:448–450CrossRefGoogle Scholar
  19. Neu C, Stein N, Keller B (2002) Genetic mapping of the Lr20-Pm1 resistance locus reveals suppressed recombination on chromosome arm 7AL in hexaploid wheat. Genome 45:737–44CrossRefPubMedGoogle Scholar
  20. Paterson AH (1996) Making genetic maps in plants. In: Paterson AH (ed) Genome mapping in plants. RG Landes and Academic, Austin and New York, pp 21–37Google Scholar
  21. Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023PubMedGoogle Scholar
  22. Rong JK, Millet E, Manisterski J, Feldman M (2000) A new powdery mildew resistance gene: Introgression from wild emmer into common wheat and RFLP-based mapping. Euphytica 115:121–126Google Scholar
  23. Saini RG, Kaur M, Singh B, Sharma S, Nanda GS, Nayar SK, Gupta AK, Nagarajan S (2002) Genes Lr48 and Lr49 for hypersensitive adult plant leaf rust resistance in wheat (Triticum aestivum L.). Euphytica 124:365–370CrossRefGoogle Scholar
  24. Schneider DM, Heun M, Fischbeck G (1991) Inheritance of the powdery mildew resistance gene Pm9 in relation to Pm1 and Pm2 of wheat. Plant Breed 107:161–164Google Scholar
  25. Schwarz G, Herz M, Huang XQ, Michalek W, Jahoor A, Wenzel G, Mohler V (2000) Application of fluorescence-based semi-automated AFLP analysis in barley and wheat. Theor Appl Genet 100:545–551Google Scholar
  26. Sheen SJ, Snyder LA (1964) Studies on the inheritance of resistance to six stem rust cultures using chromosome substitution lines of a Marquis wheat selection. Can J Genet Cytol 6:74–82Google Scholar
  27. Singrün Ch, Hsam SLK, Hartl L, Zeller FJ, Mohler V (2003) Powdery mildew resistance gene Pm22 in cultivar Virest is a member of the complex Pm1 locus in common wheat (Triticum aestivum L. em Thell.). Theor Appl Genet 106:1420–1424PubMedGoogle Scholar
  28. Szunics L, Szunics L, Vida G (1999) Changes in the race composition of the wheat powdery mildew population over the last 25 years. Novenytermeles 48:357–366Google Scholar
  29. Van Ooijen JW, Voorrips RE (2001) joinmap 3.0. Software for the calculation of genetic linkage maps. Plant Research International, Wageningen, The NetherlandsGoogle Scholar
  30. Voorrips RE (2002) mapchart: software for the graphical presentation of linkage maps and QTLs. J Hered 93:77–78CrossRefPubMedGoogle Scholar
  31. Wenzel G, Lössl A, Frei U, Mohler V, Hsam SLK, Huang XQ, Thümmler F, Zeller FJ (2000) Genomics as a tool for an efficient utilization of genetic resources using potato and wheat as examples. In: Oono K, Komatsuda T, Kadowaki K, Vaughan D (eds) Integration of biodiversity and genome technology for crop improvement. Sato Printing, Tsukuba, Japan, pp 7–10Google Scholar
  32. Young ND (1996) QTL mapping and quantitative disease resistance in plants. Annu Rev Phytopathol 34:479–501Google Scholar

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Ch. Singrün
    • 1
  • S. L. K. Hsam
    • 2
  • F. J. Zeller
    • 2
  • G. Wenzel
    • 1
  • V. Mohler
    • 1
    Email author
  1. 1.Chair of Agronomy and Plant Breeding, Department of Plant Sciences, Center for Life and Food Sciences WeihenstephanTechnical University Munich FreisingGermany
  2. 2.Division of Plant Breeding and Applied Genetics, Department of Plant Sciences, Center for Life and Food Sciences WeihenstephanTechnical University MunichFreisingGermany

Personalised recommendations