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Theoretical and Applied Genetics

, Volume 109, Issue 1, pp 146–152 | Cite as

A high-resolution map of the H1 locus harbouring resistance to the potato cyst nematode Globodera rostochiensis

  • Erin BakkerEmail author
  • Ute Achenbach
  • Jeroen Bakker
  • Joke van Vliet
  • Johan Peleman
  • Bart Segers
  • Stefan van der Heijden
  • Piet van der Linde
  • Robert Graveland
  • Ronald Hutten
  • Herman van Eck
  • Eric Coppoolse
  • Edwin van der Vossen
  • Jaap Bakker
  • Aska Goverse
Original Paper

Abstract

The resistance gene H1 confers resistance to the potato cyst nematode Globodera rostochiensis and is located at the distal end of the long arm of chromosome V of potato. For marker enrichment of the H1 locus, a bulked segregant analysis (BSA) was carried out using 704 AFLP primer combinations. A second source of markers tightly linked to H1 is the ultra-high-density (UHD) genetic map of the potato cross SH × RH. This map has been produced with 387 AFLP primer combinations and consists of 10,365 AFLP markers in 1,118 bins (http://www.dpw.wageningen-ur.nl/uhd/). Comparing these two methods revealed that BSA resulted in one marker/cM and the UHD map in four markers/cM in the H1 interval. Subsequently, a high-resolution genetic map of the H1 locus has been developed using a segregating F1 SH × RH population consisting of 1,209 genotypes. Two PCR-based markers were designed at either side of the H1 gene to screen the 1,209 genotypes for recombination events. In the high-resolution genetic map, two of the four co-segregating AFLP markers could be separated from the H1 gene. Marker EM1 is located at a distance of 0.2 cM, and marker EM14 is located at a distance of 0.8 cM. The other two co-segregating markers CM1 (in coupling) and EM15 (in repulsion) could not be separated from the H1 gene.

Keywords

Potato Cultivar AFLP Marker Bulk Segregant Analysis Nematode Resistance Potato Cyst Nematode 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

This research was financially supported by the Dutch Technology Foundation (STW) and EC grant FAIR5-PL97-2565.

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Erin Bakker
    • 1
    Email author
  • Ute Achenbach
    • 1
  • Jeroen Bakker
    • 1
    • 1
  • Joke van Vliet
    • 1
  • Johan Peleman
    • 3
  • Bart Segers
    • 3
  • Stefan van der Heijden
    • 4
  • Piet van der Linde
    • 5
  • Robert Graveland
    • 5
  • Ronald Hutten
    • 2
  • Herman van Eck
    • 2
  • Eric Coppoolse
    • 6
  • Edwin van der Vossen
    • 6
  • Jaap Bakker
    • 1
    • 1
  • Aska Goverse
    • 1
  1. 1.Laboratory of Nematology, The Graduate School of Experimental Plant SciencesWageningen UniversityWageningenThe Netherlands
  2. 2.Laboratory of Plant Breeding, The Graduate School of Experimental Plant SciencesWageningen UniversityWageningenThe Netherlands
  3. 3.Keygene NV WageningenThe Netherlands
  4. 4.Cebeco SeedsLelystadThe Netherlands
  5. 5.HZPC R and D MetslawierThe Netherlands
  6. 6.Plant Research International WageningenThe Netherlands

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