Theoretical and Applied Genetics

, Volume 108, Issue 7, pp 1385–1391 | Cite as

Genetic diversity in European winter triticale determined with SSR markers and coancestry coefficient

  • S. H. Tams
  • E. Bauer
  • G. Oettler
  • A. E. Melchinger
Original Paper

Abstract

Knowledge of the genetic diversity of a species is important for the choice of crossing parents in line and hybrid breeding. Our objective was to investigate European winter triticale using simple sequence repeat (SSR) markers and the coancestry coefficient (f) with regard to genetic diversity and grouping of germplasm. Three to five primer pairs for each of the 42 chromosomes were selected to analyse 128 European winter triticale varieties and breeding lines. SSR analysis resulted in the identification of 657 alleles with an average of 6.8 alleles per primer pair. The average polymorphism information content (PIC) for polymorphic markers was 0.54. Correlation between f and genetic similarity (GS) estimates based on Rogers’ Distance was low (rf×GS(ABDR)=0.33). The analysis of molecular variance (AMOVA) revealed that 84.7% of the total variation was found within breeding companies, and 15.3% among them. In conclusion, SSR markers from wheat and rye provide a powerful tool for assessing genetic diversity in triticale. Even though no distinct groups within the European winter triticale pool could be detected by principal co-ordinate analysis, this study provides basic information about the genetic relationships for breeding purposes.

Notes

Acknowledgements

This paper is dedicated to Prof. Dr. agr. H.H. Geiger on the occasion of his 65th birthday. The present study was supported by grants from Bundesministerium für Verbraucherschutz, Ernährung und Landwirtschaft (BMVEL) and Gemeinschaft zur Förderung der privaten deutschen Pflanzenzüchtung e.V. (GFP, grant G88/00HS). The authors gratefully acknowledge the skilled technical assistance of Angela Harmsen. We are indebted to the breeding companies for making available seeds and supplying confidential pedigree information. We thank Dr. Marion Röder, Dr. Bernd Hackauf and Dr. Viktor Korzun for providing confidential primer information.

Supplementary material

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Supplementary material (PDF 60 KB)

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • S. H. Tams
    • 1
  • E. Bauer
    • 1
  • G. Oettler
    • 1
  • A. E. Melchinger
    • 2
  1. 1.State Plant Breeding InstituteUniversity of Hohenheim StuttgartGermany
  2. 2.Institute of Plant Breeding, Seed Science and Population GeneticsUniversity of Hohenheim StuttgartGermany

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