Theoretical and Applied Genetics

, Volume 108, Issue 7, pp 1274–1284 | Cite as

Genetic diversity of oil palm (Elaeis guineensis Jacq.) germplasm collections from Africa: implications for improvement and conservation of genetic resources

  • A. Hayati
  • R. Wickneswari
  • I. Maizura
  • N. Rajanaidu
Original Paper

Abstract

A total of 723 accessions of oil palm (Elaeis guineensis Jacq.) from 26 populations representing ten countries in Africa and one Deli dura family were screened for allelic variation at seven enzyme loci from six enzyme systems using starch gel electrophoresis. On average, 54.5% of the loci were polymorphic (0.99 criterion). The average and effective number of alleles per locus was 1.80 and 1.35, respectively. Mean expected heterozygosity was 0.184, with values ranging from 0.109 (population 8, Senegal) to 0.261 (population 29, Cameroon). The genetic differentiation among populations was high (FST=0.301), indicating high genetic divergence. The calculation of FST by geographic zones revealed that the high FST was largely due to FST among populations in West Africa, suggesting diversifying selection in this region. The mean genetic distance across populations was 0.113. The lowest genetic distance (D) was observed between population 5 from Tanzania and population 7 from the Democratic Republic of the Congo (0.000) and the highest was found between population 4 from Madagascar and population 13 from Sierra Leone (0.568). The total gene flow across oil palm populations was low, with an Nm of 0.576, enhancing genetic structuring, as evident from the high FST values. UPGMA cluster analysis revealed three main clusters; the western outlying populations from Senegal and Sierra Leone were in one cluster but separated into two distinct sub-clusters; the eastern outlying populations from Madagascar were in one cluster; the populations from Angola, Cameroon, The Democratic Republic of the Congo, Ghana, Tanzania, Nigeria and Guinea were in one cluster. The Deli dura family seems to be closely related to population 6 from Guinea. Oil palm populations with high genetic diversity—i.e. all of the populations from Nigeria, Cameroon and Sierra Leone, population 6 of Guinea, population 1 of Madagascar and population 2 of Senegal should be used in improvement programmes, whereas for conservation purposes, oil palm populations with high allelic diversity (Ae), which include populations 22 and 29 from Cameroon, populations 39 and 45 from Nigeria, population 6 from Guinea, populations 5 and 13 from Sierra Leone and population 1 from Madagascar should be selected for capturing as much genetic variation as possible.

References

  1. Arasu NT (1985) Genetic variation for fatty acid composition in the oil palm (Elaeis guineensis Jacq.). PhD thesis, University of Birmingham, UKGoogle Scholar
  2. Byrne M, Parrish TL, Moran GF (1998) Nuclear RFLP diversity in Eucalyptus nitens. Heredity 81:225–233CrossRefGoogle Scholar
  3. Choong CY, Shah FH, Rajanaidu N, Zakri AH (1996) Isoenzyme variation of Zairean oil palm (Elaeis guineensis Jacq.) germplasm collection. Elaeis 8:45–53Google Scholar
  4. Clayton JW, Tretiak DN (1972) Amine-citrate buffers for pH control in starch gel electrophoresis. J Fish Res Board Can 29:1169–1172Google Scholar
  5. Cottrell JE, White MS (1995) The use of isozyme genetic markers to estimate the rate of outcrossing in a Sitka spruce (Picea sitchensis (Bong.) Carr.) seed orchard in Scotland. New For 10:111–122Google Scholar
  6. Crow JF, Kimura M (1970) An introduction to population genetic theory. Harper and Row, New YorkGoogle Scholar
  7. Delorenzo RJ, Ruddle FH (1969) Genetic control of two electrophoretic variants of glucosephosphate isomerase in the mouse (Mus musculus). Biochem Genet 3:151PubMedGoogle Scholar
  8. Eguiarte LE, Perez-Nasser N, Pinero D (1992) Genetic structure, outcrossing rate and heterosis in Astrocaryum mexicanum (tropical palm): implications for evolution and conservation. Heredity 69:217–228Google Scholar
  9. Fine IH, Costello LA (1963) The use of starch electrophoresis in dehydrogenase studies. In: Colowick SP, Kaplan NO (eds) Methods in enzymology. vol 6. Academic Press, New York, pp 958–972Google Scholar
  10. Ghesquiere M (1985) Enzyme polymorphism in oil palm (Elaeis guineensis Jacq.). II. Variability and genetic structure of seven origins of oil palm. Oleagineux 40:529–540Google Scholar
  11. Granger AR, Clarke GR, Jackson JF (1993) Sweet cherry cultivar identification by leaf isozyme polymorphism. Theor Appl Genet 86:458–464Google Scholar
  12. Hall P, Orrell LC, Bawa KS (1994) Genetic diversity and mating system in a tropical tree, Carapa guianensis (Meliaceae). Am J Bot 81:1104–1111Google Scholar
  13. Hamrick JL, Godt MJW (1989) Allozyme diversity in plant species. In: Brown AHD, Clegg MJ, Kahler AL, Weir BS (eds) Plant population genetics, breeding and genetic resources. Sinauer Assoc, Sunderland, pp 43–63Google Scholar
  14. Hamrick JL, Murawski DA (1991) Levels of allozyme diversity in populations of uncommon neotropical tree species. J Trop Ecol 7:395–399Google Scholar
  15. Hamrick JL, Mitton JB, Linhart YB (1981) Levels of genetic variation in trees: influences of life history characteristics. In: Conkle MT (ed) Proc Symp Isozymes N Am For Trees For Insects. USDA, Forest Service, General Technical Report, PSW-48, pp 35–41Google Scholar
  16. Hardon JJ (1974) Oil palm. In: Leon J (ed) Handbook of plant introduction in tropical crops. FAO Agricultural Studies, no. 93, RomeGoogle Scholar
  17. Hartley CWS (1988) The oil palm (Elaeis guineensis Jacq.). Longman Scientific and Technical Publ, New YorkGoogle Scholar
  18. Hayati A (2002) Isozyme variations study in African oil palm (Elaeis guineensis Jacq.) germplasm collections. MSc thesis, Universiti Kebangsaan MalaysiaGoogle Scholar
  19. Kularatne RS (2000) Assessment of genetic diversity in natural oil palm (Elaeis guineensis Jacq.) populations using amplified fragment length polymorphic markers. PhD thesis, Universiti Kebangsaan MalaysiaGoogle Scholar
  20. Lopez CR, Dosrciss SF, Ferreira MA, Moretzsohnn MC (1992) Genetics of the genus Acrocomia (palmae). III. Microgeographical genetic variability in isozyme frequencies. J Genet Breed 46:9–13Google Scholar
  21. Loveless MD (1992) Isozyme variation in tropical trees: patterns of genetic organization. New For 6:67–94Google Scholar
  22. Loveless MD, Hamrick JL (1984) Ecological determinants of genetic structure in plant populations. Annu Rev Ecol Syst 15:65–95CrossRefGoogle Scholar
  23. Maizura I (1999) Genetic variability of oil palm (Elaeis guineensis Jacq.) germplasm collection using RFLP markers. PhD thesis, Universiti Kebangsaan MalaysiaGoogle Scholar
  24. Maxted N, Ford-Lloyd BV, Hawkes JG (1997) Plant genetic conservation, the in situ approach. Chapman and Hall, LondonGoogle Scholar
  25. Moran CF (1992) Pattern of genetic diversity in Australian tree species. New For 6:49–66Google Scholar
  26. Müller-Starck G, Baradat PH, Bergmann F (1992) Genetic variation within European tree species. New For 6:23–47Google Scholar
  27. Nei M (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89:583–590Google Scholar
  28. Poulik MD (1957) Starch gel electrophoresis in a discontinuous system of buffers. Nature 180:1477–1479Google Scholar
  29. Purba AR, Noyer JL, Baudouin L, Perrier X, Hamon S, Lagoda PJL (2000) A new aspect of genetic diversity of Indonesian oil palm (Elaeis guineensis Jacq.) revealed by isoenzyme and AFLP markers and its consequences for breeding. Theor Appl Genet 101:956–961CrossRefGoogle Scholar
  30. Purseglove JW (1975) Tropical crops, monocotyledons. The English Language Book Society and Longman Scientific Publ, LondonGoogle Scholar
  31. Rajanaidu N (1980) Variation in the natural population of oil palm (Elaeis guineensis Jacq.). PhD thesis, University of Birmingham, UKGoogle Scholar
  32. Rajanaidu N, Jalani BS (1994) Oil palm genetic resources- collection, evaluation, utilization and conservation. Presented at PORIM Colloquium on Oil Palm Genetic Resources. PORIM, Bangi, Malaysia.Google Scholar
  33. Rajanaidu N, Rao V, Abdul Halim H, Ong ASH (1989) Genetic resources—new development in oil palm breeding. Elaeis 1:1-10Google Scholar
  34. Rajanaidu N, Maizura I, Cheah SC (2000) Screening of oil palm natural populations using RAPD and RFLP molecular markers. In: Rajanaidu N, Ariffin D (eds) Proc Int Symp Oil Palm Genet Resources Utilization, pp AA1–AA28Google Scholar
  35. Rao V (1987) Genetic variation in population of oil palm (Elaeis guineensis Jacq.). PhD thesis, University of Birmingham, UKGoogle Scholar
  36. Rees AR (1965) Evidence for the African origin of the oil palm. Principes 9:30Google Scholar
  37. Sapurah R (1990) Isozyme variation in oil palm (Elaeis guineensis Jacq.) germplasm from Nigeria (in Malay). MSc thesis, Universiti Kebangsaan MalaysiaGoogle Scholar
  38. Shah FH, Lim SN (1996) Use of microsatellite in the determination of genetic variation and genetic relationship between various oil palm populations. In: PORIM (ed) Proc PORIM Int Palm Oil Congr. PORIM, Bangi, pp 568–582Google Scholar
  39. Shah FH, Zuliaha S, Omar R (1993) Polymorphism of rDNA locus of oil palm: a preliminary investigation. Proc Biochem Soc Symp 17:81–84Google Scholar
  40. Shah FH, Rashid O, Simons AJ, Dunsdon A (1994) The utility of RAPD markers for the determination of genetic variation in oil palm (Elaeis guineensis). Theor Appl Genet 89:713–718Google Scholar
  41. Shapcott A (1998) The genetics of Ptychosperma bleeseri, a rare palm from the Northern Territory, Australia. Biol Conserv 85:203–209CrossRefGoogle Scholar
  42. Sneath PHA, Sokal RR (1973) Numerical taxonomy. WH Freeman Press, San FranciscoGoogle Scholar
  43. Soltis DE, Soltis PS (1989) Polyploidy, breeding systems and genetic differentiation in homosporous pteridophytes. In: Soltis DE, Soltis PS (eds) Isozymes in plant biology. Dioscorides Press, Portland, Ore., pp 241–258Google Scholar
  44. Soltis DE, Haufler CH, Darrow DC, Gastony GJ (1983) Starch gel electrophoresis of ferns: a compilation of grinding buffers, gel and electrode buffers and staining schedules. Am Fern J 73:9-27Google Scholar
  45. Spencer N, Hopkinson DA, Harris H (1964) Phosphoglucomutase polymorphism in man. Science 5:204–242Google Scholar
  46. Tanksley SD (1979) Linkage—chromosomal association and expression of Adh-1 and Pgm-1 in tomato. Biochem Genet 17:1159–1167PubMedGoogle Scholar
  47. Wickneswari R, Norwati M (1991) Techniques for starch gel electrophoresis of enzymes from Acacias. In: Carron LT, Aken KM (eds) Breeding technologies for tropical acacias, ACIAR Proc No. 37, pp 85–100Google Scholar
  48. Wickneswari R, Norwati M (1993) Genetic diversity of natural populations of Acacia auriculiformis. Aust J Bot 41:65–77Google Scholar
  49. Wickneswari R, Siti Salwana H, Norwati M, Nur Supardi MN, Aminuddin M (2002) Genetic diversity in potential seed sources of Calamus manan miq. in Peninsular Malaysia. Malays Appl Biol 31:49–58Google Scholar
  50. Wright S (1931) Evolution in Mendelian populations. Genetics 16:97–159Google Scholar
  51. Wright S (1965) The interpretation of genetic structure by F-statistics with special regard to systems of mating. Evolution 19:355Google Scholar
  52. Yeh FC, Boyle T (1999) popgene version 1.32. The user-friendly software for population genetic analysis. University of Alberta and CIFOR, Calgary, Alta.Google Scholar
  53. Zeven AC (1964) On the origin of the oil palm. Grana Palynol 5:50Google Scholar
  54. Zeven AC (1967) The semi-wild oil palm and its industry in Africa. Agric Res Rep no. 698Google Scholar
  55. Zheng YQ, Ennos RA (1999) Genetic variability and structure of natural and domesticated populations of Caribbean pine (Pinus caribaea Morelet). Theor Appl Genet 98:765–771CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • A. Hayati
    • 1
  • R. Wickneswari
    • 1
  • I. Maizura
    • 2
  • N. Rajanaidu
    • 2
  1. 1.School of Environmental and Natural Resources Sciences, Faculty of Science and TechnologyUniversiti Kebangsaan MalaysiaBangiMalaysia
  2. 2.Malaysian Palm Oil Board (MPOB)Kuala LumpurMalaysia

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