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Screening interspecific hybrids of Populus (P. ciliata × maximowiczii) using AFLP markers

Abstract

Hybrids of Populus ciliata × maximowiczii are very vigorous and outperform both the parents in growth performance and yield. Genetic evaluation of 24 of these interspecific hybrids along with the two mother trees (Populus ciliata), and five male-parent (Populus maximowiczii) genotypes was carried out using the AFLP marker assay. Eight AFLP primer combinations detected 428 markers, of which 280 (66%) were polymorphic. Genetic relationships within the samples were evaluated by generating the similarity matrix based on Jaccard’s coefficient. The phenetic dendrograms, as well as the PCO plots, separated the hybrids and the two parent species into three distinct clusters. The hybrids grouped closer to the P. ciliata (female parent) cluster as compared to the P. maximowiczii (male parent) cluster. The hybrid cluster contained internal groupings, which correlated to some extent with growth performance. The four best performing hybrids (42m1, 65m1, 23m2, Cm2-5-20/91) formed a distinct sub-cluster. Data from a single primer combination was sufficient for distinguishing the hybrids from the parents and assigning paternity. The hybrids showed 22 markers that were absent in P. ciliata but were monomorphically present in all the hybrids, suggesting outcrossing and common paternity. Further, these 22 markers were found in all the P. maximowiczii genotypes confirming it as the male parent. These male-specific markers can be converted to SCAR markers and used for rapid screening of the P.ciliata × maximowiczii hybrids. The primer combination E-AAC × M-CAA was identified as most suitable for ascertaining true hybridity. AFLP proves to be a useful tool for screening of P. ciliata × maximowiczii hybrids at the early stages of development.

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Reference

  1. Bradshaw HD, Villar M, Watson BD, Otto KG, Stewart S, Stettler RF (1994) Molecular genetics of growth and development in Populus. III. A genetic linkage map of a hybrid poplar composed of RFLP, STS, and RAPD markers. Theor Appl Genet 89:167–178

  2. Cervera MT, Villar M, Faivre-Rampant P, Goue MC, Van Montagu M, Boerjan W (1997) Applications of molecular marker technologies in Populus breeding. In: Klopfenstein NB, Chun YW, Kim MS, Ahuja MR (eds) Micropropagation, genetic engineering, and molecular biology of Populus. Rocky Mountain Forest and Range Experiment Station, Fort Collins, Colorado, pp 101–115

  3. Doyle JJ, Doyle JL (1990) Isolation of plants DNA from fresh tissue. Focus 12:13–15

  4. Ellis RP, McNicol JW, Baird E, Booth A, Lawrence P, Thomas B, Powell W (1997) The use of AFLPs to examine genetic relatedness in Barley. Mol Breed 3:359–369

  5. FAO (1979) Poplars and willows. FAO Forestry Series No. 10

  6. Felsenstein J (1985) Confidence limits on phylogenies: an approach using bootstrap. Evolution 39:783–791

  7. Gilbert KG, Garton S, Karam MA, Arnold GM, Karp A, Edwards KJ, Cooke DT, Barker JHA (2002) A high degree of genetic diversity is revealed in Isatis spp. (dyer’s woad) by amplified fragment length polymorphism (AFLP). Theor Appl Genet 104:1150–1156

  8. Highton R (1993) The relationship between the number of loci and the statistical support for the topology of UPGMA trees obtained from genetic distance data. Mol Phylog Evol 2:337–343

  9. Jaccard P (1908) Nouvelles researches sur la distribution florale. Bull Soc Vaud Sci Nat 44:223–270

  10. Khosla PK, Khurana DK (1982) Evolution of the genus Populus L. and systematic placement of P. ciliata WALL. ex. ROYLE. J Tree Sci 1:81–87

  11. Khurana DK (2002) Genetic diversity in poplars for sustainable development. In: Vij SP, Kondu K, Sharma ML, Gupta A (eds) Plant genetic diversity: exploration, evaluation, conservation. Affliated East-West Press, New Delhi, pp 181–187

  12. Khurana DK, Thakur S (1995) P. ciliata × maximowiczii: preliminary report on a potential hybrid for mid west Himalayan Zone. Indian Forester 121:802–806

  13. Kopp RF, Smart LB, Maynard CA, Tuskan GA, Abrahamson LP (2002) Predicting within-family variability in juvenile height growth of Salix based upon similarity among parental AFLP fingerprints. Theor Appl Genet 105:106–112

  14. Krauss SL (2000) Patterns of mating in Persoonia mollis (Proteaceae) revealed by an analysis of paternity using AFLP: implications for conservation. Aust J Bot 48:349–356

  15. Lima MLA, Garcia AAF, Oliveira KM, Matsuoka S, Arizono H, de Souza Jr. CL, de Souza AP (2002) Analysis of genetic similarity detected by AFLP and coefficient of parentage among genotypes of sugar cane (Saccharum spp.) Theor Appl Genet 104:30–38

  16. Promnitz LC, Wray PH (1976) Rapid selection techniques for identifying superior clones. USDA For Serv North Cent Res Stn Gen Tech Rep NC-21: pp 25–31

  17. Rahman MH, Rajora OP (2002) Microsatellite DNA fingerprinting, differentiation, and genetic relationships of clones, cultivars, and varieties of six poplar species from three sections of the genus Populus. Genome 45:1083–1094

  18. Rajora OP (1988) Allozymes as aids for identification of some Populus maximowiczii Henry clonal varieties. Biochem Syst Ecol 16:635–640

  19. Rajora OP, Dancik BP (1995) Chloroplast DNA variation in Populus. I. Intraspecific restriction fragment diversity within Populus deltoides, P. nigra and P. maximowiczii. Theor Appl Genet 90:317–323

  20. Rajora OP, Rahman MH (2003) Microsatellite DNA and RAPD fingerprinting, identification and genetic relationships of hybrid poplar (Populus × canadensis) cultivars. Theor Appl Genet 106:470–477

  21. Rohlf FJ (1998) NTSYS-pc. Numerical taxonomy and multivariate analysis system. Version 2.0. Exeter Publications, New York

  22. Singh A, Negi MS, Rajagopal J, Bhatia S, Tomar UK, Srivastava PS, Lakshmikumaran M (1999) Assessment of genetic diversity in Azadirachta indica using AFLP markers. Theor Appl Genet 99:272–279

  23. Singh A, Chaudhury A, Srivastava PS, Lakshmikumaran M (2002) Comparision of AFLP and SAMPL markers for assessment of intra-population genetic variation in Azadirachta indica A. Juss. Plant Sci 162:17–25

  24. Sneath PHA, Sokal RR (1973) Numerical taxonomy. WH Freeman, San Francisco, California

  25. Sokal RR, Michener CD (1958) A statistical method for evaluating systematic relationships. Univ Kansas Sci Bull 38:1409–1438

  26. Steiger DL, Nagai C, Moore PH, Morden CW, Osgood RV, Ming R (2002) AFLP analysis of genetic diversity within and among Coffea arabica cultivars. Theor Appl Genet 105:209–215

  27. Van Droogenbroeck B, Breyne P, Goetghebeur P, Romeijen-Peeteers, Kyndt T, Gheysen G (2002) AFLP analysis of the genetic relationships among papaya and its wild relatives (Caricaceae) from Ecuador. Theor Appl Genet 105:289–297

  28. VanToai TT, Peng J, St Martin S (1996) Using AFLP markers to determine the contribution of parental genomes during recurrent selection. Soybean Genet Newslett 23:214–216

  29. Yap IV, Nelson RJ (1995) WINBOOT, a program for performing bootstrap analysis of binary data to determine the confidence limits of UPGMA-based dendrograms. IRRI Discussion Paper Series No. 14, Intern Rice Res Inst, Los Baños, The Phillipines

  30. Zabeau M, Vos P (1993) Selective restriction fragment amplification: a general method for DNA fingerprinting. European Patent Application of 92402629, Publ No. 0534858A1

  31. Zsuffa L (1975) A summary review of interspecific breeding in the genus Populus L. Proc 14th Meeting of the Canadian Tree Improvement Association, Part 2. Fredericton, NB 1973. Canadian Forestry Service, Ottawa, Ontario, pp 107–123

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Acknowledgements

Authors thank Dr. R. K. Pachauri, Director-General, TERI, and Dr. T.P. Singh, Director, TERI-School of Advanced Studies, for their support and encouragement. We are grateful to Dr. Stefano Bissoffi and Dr. Pierre Perinet for sending us the P. maximowiczii leaf material used in this study. The Senior Research Fellowship to N. Chauhan and V. Sabharwal from Council of Scientific and Industrial Research (CSIR) is duly acknowledged.

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Correspondence to M. Lakshmikumaran.

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Communicated by H.F. Liskens

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Chauhan, N., Negi, M.S., Sabharwal, V. et al. Screening interspecific hybrids of Populus (P. ciliata × maximowiczii) using AFLP markers. Theor Appl Genet 108, 951–957 (2004). https://doi.org/10.1007/s00122-003-1508-5

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Keywords

  • Populus
  • Genetic Similarity
  • AFLP Marker
  • Mother Tree
  • Scar Marker