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Theoretical and Applied Genetics

, Volume 108, Issue 5, pp 842–850 | Cite as

Genomic characterization of the S-adenosylmethionine decarboxylase genes from soybean

  • Ai-Guo Tian
  • Jing-Yun Zhao
  • Jin-Song Zhang
  • Jun-Yi Gai
  • Shou-Yi Chen
Original Paper

Abstract

A full-length gene GmSAMDC1, encoding the S-adenosylmethionine decarboxylase (SAMDC), a key enzyme involved in polyamine biosynthesis, was identified from soybean expressed sequence tags and was characterized. GmSAMDC1 encoded a peptide of 355 amino acids. When compared with other plant SAMDCs, the GmSAMDC1 protein had several highly conserved regions including a putative pro-enzyme cleavage site and a PEST sequence. The 5′ leader sequence of the the GmSAMDC1 mRNA contained two additional open reading frames (ORFs), which may regulate the translational process. The genomic sequence of the GmSAMDC1 gene contained three introns in the 5′ leader sequence, but no intron in the 3′-UTR or the main pro-enzyme ORF. A simple sequence repeat (SSR) was found in intron 2, and the GmSAMDC1 gene was mapped to linkage group D1 using this SSR. The genomic organization of the GmSAMDC1 gene in the subgenus Glycine and the subgenus Soja was found to be different by Southern-blot and PCR analysis. A pseudogene, GmSAMDC2, was also identified. This gene contained no intron and lost its two uORFs. Northern-blot analysis showed that the GmSAMDC1 gene expression was induced by salt, drought and cold, but not induced by wounding; suggesting that the gene was implicated in response to multiple-stress conditions.

Keywords

Putrescine Leader Sequence Soybean Cultivar Pest Sequence SAMDC Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

This work was supported by National Key Basic Research Special Funds, PR, China (G1998010209 and 2002CB111301).

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Ai-Guo Tian
    • 1
  • Jing-Yun Zhao
    • 2
  • Jin-Song Zhang
    • 1
  • Jun-Yi Gai
    • 3
  • Shou-Yi Chen
    • 1
  1. 1.Plant Biotechnology Laboratory, Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijingChina
  2. 2.Shanxi Agricultural UniversityTaiguChina
  3. 3.National Center for Soybean Improvement, Soybean Research InstituteNanjing Agricultural UniversityNanjingChina

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