Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Genetic mapping in (Populus tomentosa × Populus bolleana) and P. tomentosa Carr. using AFLP markers

  • 248 Accesses

  • 36 Citations

Abstract

The AFLP genetic linkage maps for two poplar cultivars were constructed with the pseudo-test-cross mapping strategy. The hybrids were derived from an interspecific backcross between the female hybrid clone “TB01” (Populus tomentosa × Populus bolleana) and the male clone “LM50” (P. tomentosa). A total of 782 polymorphic fragments were obtained with a PCR-based strategy using 49 enzyme-nested (EcoRI/MseI) primer combinations. Six hundred and thirty two of these fragments segregated in a 1:1 ratio (P<0.01), indicating that these DNA polymorphisms are heterozygous in one parent and null in the other. The linkage analysis was performed using Mapmaker version 3.0 with LOD 5.0 and a maximum recombination fraction (θ) of 0.3. Map distances were estimated using the Kosambi mapping function. In the framework map for “LM50” (P. tomentosa), 218 markers were aligned in 19 major linkage groups. The linked loci spanned approximately 2,683 cM of the poplar genome, with an average distance of 12.3 cM between adjacent markers. For “TB01” (P. tomentosa × P. bolleana), the analysis revealed 144 loci, which were mapped to 19 major linkage groups and covered about 1,956 cM, with an average distance of 13.6 cM between adjacent markers. These maps covered about 87% and 77% of the estimated genome size of parents “LM50” and “TB01”, respectively. The maps developed in this study lay an important foundation for future genomics research in poplar, providing a means for localizing genes controlling economically important traits in P. tomentosa.

This is a preview of subscription content, log in to check access.

References

  1. Bert PF, Charmet G, Sourdilli P, Hayward MD, Balfourier F (1999) A high-density molecular map for ryegrass (Lolium perenme) using AFLP markers. Theor Appl Genet 99:445–452

  2. Bradshaw HD, Stettler RF (1994) Molecular genetics of growth and development in Populus. II. Segregation distortion due to genetic load. Theor Appl Genet 89:551– 558

  3. Bradshaw HD, Stettler RF (1995) Molecular genetics of growth and development in Populus. IV. Mapping QTLs with large effects on growth, form, and phenology traits in a forest tree. Genetics 139:963–973

  4. Bradshaw HD, Villar M, Watson BD, Otto KG, Stewart S, Stettler RF (1994) Molecular genetics of growth and development in Populus. III. A genetic linkage map of a hybrid poplar composed of RFLP, STS and RAPD markers. Theor Appl Genet 89:167–178

  5. Cervera MT, Storme Véronique, Ivens Bart, Gusmão Jaqueline, Liu Ben H, Hostyn Vanessa, Slycken Jos Van, Montagu Marc Van, Boerjan Wout (2001) Dense genetic linkage maps of three Populus species (Populus deltoides, P. nigra and P. trichocarpa) based on AFLP and microsatellite markers. Genetics 158:787–809

  6. Devey ME, Delfino-Mix A, Kinloch BB, Neale DB (1995) Random amplified polymorphic DNA markers tightly linked to a gene for resistance to white pine blister rust in sugar pine. Proc Natl Acad Sci 92:2066–2070

  7. Grattapaglia D, Sederoff R (1994) Genetic linkage maps of Eucalyptus grandis and Eucalyptus urophylla using a pseudo-test-cross: mapping strategy and RAPD markers. Genetics 137:1121–1137

  8. Grattapaglia D, Bertolucci FLG, Sederoff RR (1995) Genetic mapping of QTLs controlling vegetative propagation in Eucalyptus grandis and E. urophylla using a pseudo-testcross mapping strategy and RAPD markers. Theor Appl Genet 144:1205–1214

  9. Hulbert SH, Ilott TW, Legg EJ, Lincoln SE, Lander ES, Michelmore RW (1988) Genetic analysis of the fungus, Bremia lactucae, using restriction fragment length polymorphisms. Genetics 120:947–958

  10. Kuang H, Richardson T, Carson S, Wilcox P, Bongarten B (1999) Genetic analysis of inbreeding depression in plus tree 850.55 of Pinus radiata D. Don. I. Genetic map with distorted markers. Theor Appl Genet 98:697–703

  11. Lincoln S, Daly M, Lander E (1992) Constructing genetic maps with MAPMAKER/EXP 3.0. Whitehead Institute Technical Report, 3rd edn. Whitehead Institute, Cambridge, Massachusetts

  12. Liu Z, Furnier GR (1993) Inheritance and linkage of allozymes and restriction fragment length polymorphisms in trembling aspen. J Hered 84:419–424

  13. Lu ZX, Sosinski B, Reighard GL, Baird WV, Abbott AG (1998) Construction of a genetic linkage map and identification of AFLP markers for resistance to root-knot nematodes in peach rootstocks. Genome 41:199–207

  14. Marques CM, Araujo JA, Ferreira, Whetten R, O’Malley DM, Liu BH, Sederoff R (1998) AFLP genetic maps of Eucalyptus globulus and E. tereticornis. Theor Appl Genet 96:727–737

  15. Marques CM, Vasquez-Kool J, Carocha VJ, Ferreira JG, O’Malley DM, Liu BH, Sederoff R (1999) Genetic dissection of vegetative propagation traits in Eucalyptus tereticornis and E. globules. Theor Appl Genet 99:936–946

  16. Murray MG, Thompson WF (1980) Rapid isolation of high-molecular-weight plant DNA. Nucleic Acids Res 8:4321–4325

  17. Plomion C, O’Malley DM, Durel CE (1995) Genomic analysis in maritime pine (Pinus pinaster). Comparison of two RAPD maps using Selfed and open-pollinated seeds of the same individuals. Theor Appl Genet 90:1028–1034

  18. Remington DL, Whetten RW, Liu BH, O’Malley DM (1999) Construction of an AFLP genetic map with nearly complete genome coverage in Pinus taeda. Theor Appl Genet 98:1279–1292

  19. Sewell MM, Davis MF, Tuskan GA, Wheeler NC, Elam CC, Bassoni DL, Neale DB (2002) Identification of QTLs influencing wood property traits in loblolly pine (Pinus taeda L.). II. Chemical wood properties. Theor Appl Genet 104:214–222

  20. Staub JE, Serquen FC (1996) Genetic markers, map construction, and their application in plant breeding. Hortscience 31:729–740

  21. Tixier MH, Sourdille RM, Leroy P, Bernard M (1997) Detection of wheat microsatellites using a non-radioactive silver-nitrate staining method. J Genet Breed 51:175–177

  22. Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414

  23. Whitkus R (1998) Genetics of adaptive radiation in Hawaiian and Cook islands species of tetramolopium (Asteraceae). II. Genetic linkage map and its implications for interspecific breeding barriers. Genetics 150:1209–1216

  24. Williams CG, Goodman MM, Stuber CW (1995) Comparative recombination distances among Zea mays L. inbreds, wide crosses and interspecific hybrids. Genetics 141:1573–1581

  25. Wu RL, Han YF, Hu JJ, Fang JJ, Li L, Li ML, Zeng ZB (2000) An integrated genetic map of Populus deltoids based on amplified fragment length polymorphisms. Theor Appl Genet 100:1249–1256

  26. Yin TM, Huang MR, Wang Mx, Zhu LH, Zeng ZB, Wu RL (2001) Preliminary interspecific genetic maps of the Populus genome constructed from RAPD markers. Genome 44:602–609

  27. Zamir D, Tadmor Y (1986) Unequal segregation of nuclear genes in plants. Bot Gaz 147:355–358

  28. Zamir D, Tanksley SD, Jones RA (1982) Haploid selection for low-temperature tolerance of tomato pollen. Genetics 101:129–137

  29. Zhang DQ, Zhang ZY, Yang K, Tian L (2002) Segregation of AFLP Markers in a (Populus tomentosa × P. bolleana) × P. tomentosa Carr. BC1 Family. Forestry Studies in China 4:21–26

  30. Zhu ZT, Zhang ZY (1997) Advances in the triploid breeding program of Populus tomentosa in China. J Beijing For University 6:1–8

Download references

Acknowledgements

The authors thank Professor Zheng Yongqi for critical reading of the manuscript, Professor Weng Yuejin for allowing us to use the laboratory to carry out the AFLP analysis, and Professor Su Xiaohua and Dr. Song Wan for discussing the experiments. This work was supported by a grant from the Project of the National Natural Science Foundation of China (No. 30170780).

Author information

Correspondence to D. Zhang.

Additional information

Communicated by O. Savolainen

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Zhang, D., Zhang, Z., Yang, K. et al. Genetic mapping in (Populus tomentosa × Populus bolleana) and P. tomentosa Carr. using AFLP markers. Theor Appl Genet 108, 657–662 (2004). https://doi.org/10.1007/s00122-003-1478-7

Download citation

Keywords

  • Linkage Group
  • Populus
  • Segregation Distortion
  • AFLP Marker
  • Unlinked Marker