Theoretical and Applied Genetics

, Volume 108, Issue 4, pp 639–643 | Cite as

A novel locus for clubroot resistance in Brassica rapa and its linkage markers

  • M. HiraiEmail author
  • T. Harada
  • N. Kubo
  • M. Tsukada
  • K. Suwabe
  • S. Matsumoto


An inbred turnip (Brassica rapa syn. campestris) line, N-WMR-3, which carries the trait of clubroot resistance (CR) from a European turnip, Milan White, was crossed with a clubroot-susceptible doubled haploid line, A9709. A segregating F3 population was obtained by single-seed descent of F2 plants and used for a genetic analysis. Segregation of CR in the F3 population suggested that CR is controlled by a major gene. Two RAPD markers, OPC11-1 and OPC11-2, were obtained as candidates of linkage markers by bulked segregant analysis. These were converted to sequence-tagged site markers, by cloning and sequencing of the polymorphic bands, and named OPC11-1S and OPC11-2S, respectively. The specific primer pairs for OPC11-1S amplified a clear dominant band, while the primer pairs for OPC11-2S resulted in co-dominant bands. Frequency distributions and statistical analyses indicate the presence of a major dominant CR gene linked to these two markers. The present marker for CR was independent of the previously found CR loci, Crr1 andCrr2. Genotypic distribution and statistical analyses did not show any evidence of CR alleles on Crr1 andCrr2 loci in N-WMR-3. The present study clearly demonstrates that B. rapa has at least three CR loci. Therefore, the new CR locus was named Crr3. The present locus may be useful in breeding CR Chinese cabbage cultivars to overcome the decay of present CR cultivars.


Chinese Cabbage Linkage Marker Clubroot Resistance Clubroot Disease Clubroot Resistance Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This investigation was partly supported by a Grant-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan (No. 1340006) to M.H.


  1. Arumuganathan K, Earle ED (1991) Nuclear DNA content of some important plant species. Plant Mol Biol Report 9:211–215Google Scholar
  2. Axelsson T, Shavorskaya O, Lagercrantz U (2001) Multiple flowering time QTLs within several Brassica species could be the result of duplicated copies of one ancestral gene. Genome 44:856–864CrossRefPubMedGoogle Scholar
  3. Buczacki ST, Toxopeus H, Mattusch P, Johnston TD, Dixon GR, Hobolth LA (1975) Study of physiologic specialization in Plasmodiophora brassicae: proposals for rationalization through an international approach. Trans Br Mycol Soc 65:295–303Google Scholar
  4. Crute IR, Gray AR, Crips P, Buczacki ST (1980) Variation in Plasmodiophora brassicae and resistance to clubroot disease in brassicas and allied crops—a critical review. Plant Breed Abstr 50:91–104Google Scholar
  5. Figdore SS, Ferreira ME, Slocum MK, Williams PH (1993) Association of RFLP markers with trait loci affecting clubroot resistance and morphological characters in Brassica oleracea L. Euphytica 69:33–44Google Scholar
  6. Fuchs H, Sacristán MD (1996) Identification of a gene in Arabidopsis thaliana controlling resistance to clubroot (Plasmodiophora brassicae) and characterization of the resistance response. Mol Plant-Microbe Interact 9:91–97Google Scholar
  7. Grandclément C, Thomas G (1996) Detection and analysis of QTLs based on RAPD markers for polygenic resistance to Plasmodiophora brassicae Woron in Brassica oleracea L. Theor Appl Genet 93:86–90CrossRefGoogle Scholar
  8. Huang N, Angeles ER, Domingo J, Magpantay G, Singh S, Zhang G, Kumaravadivel N, Bannett J, Khush GS (1997) Pyramiding of bacterial blight resistance genes in rice: marker-assisted selection using RFLP and PCR. Theor Appl Genet 95:313–320Google Scholar
  9. Ikegami H, Ito T, Imuro Y, Naiki T (1981) Growth of Plasmodiophora brassicae in the root and callus of Chinese cabbage. In: Talekar NS, Griggs TD (eds) Chinese cabbage. Asian Vegetable Research and Development Center, Tainan, pp 81–90Google Scholar
  10. Karling JS (1968) The plasmodiophorales. Hafner, New YorkGoogle Scholar
  11. Kikuchi M, Ajisada H, Kuginuki Y, Hirai M (1999) Conversion of RAPD markers for a clubroot-resistance gene of Brassica rapa into Sequence-tagged sites (STSs). Breed Sci 49:83–89Google Scholar
  12. Kuginuki Y, Ajisaka H, Yui M, Yoshikawa H, Hida K, Hirai M (1997) RAPD markers linked to a clubroot-resistance locus in Brassica rapa L. Euphytica 98:149–154CrossRefGoogle Scholar
  13. Kuginuki Y, Yoshikawa H, Hirai M (1999) Variation in virulence of Plasmodiophora brassicae in Japan tested with clubroot-resistant cultivars of Chinese cabbage (Brassica rapa L. ssp. pekinensis). Eur J Plant Pathol 105:327–332CrossRefGoogle Scholar
  14. Lagercrantz U, Putterill J, Coupland G, Lydiate D (1996) Comparative mapping in Arabidopsis and Brassica, fine scale genome collinearity and congruence of genes controlling flowering time. Plant J 9:13–20PubMedGoogle Scholar
  15. Landry BS, Lincoln SE, Etoh T (1992) A genetic map for Brassica oleracea based on RFLP markers detected with expressed DNA sequences and mapping of resistance genes to race 2 of Plasmodiophora brassicae (Woronin). Genome 35:409–420Google Scholar
  16. Li G, Gao M, Yang B, Quiros CF (2003) Gene for gene alignment between the Brassica and Arabidopsis genomes by direct transcriptome mapping. Theor Appl Genet 107:168–180PubMedGoogle Scholar
  17. Matsumoto E, Yasui C, Ohi M, Tsukada M (1998) Linkage analysis of RFLP markers for clubroot resistance and pigmentation in Chinese cabbage (Brassica rapa ssp. pekinensis). Euphytica 104:79–86Google Scholar
  18. Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832PubMedGoogle Scholar
  19. Murray MG, Thompson WF (1980) Rapid isolation of high-molecular-weight plant DNA. Nucleic Acids Res 10:4321–4325Google Scholar
  20. Osborn TC, Kole C, Parkin IAP, Sharpe AG, Kuiper M, Lydiate DJ, Trick M (1997) Comparison of flowering time genes in Brassica rapa, B. napus and Arabidopsis thaliana. Genetics 146:1123–1129PubMedGoogle Scholar
  21. Otani, F, Maruyama S, Tsukada M, Nagase Y (1982) Studies on the breeding of clubroot resistance in Chinese cabbage and Chinese mustard II. Process of breeding the clubroot resistant line of Nozawana, a variety of Chinese mustard, and its characteristics (in Japanese with English summary). Bull Nagano Veg Ornam Crop Exp Sta Jpn 2:1–8Google Scholar
  22. Suwabe K, Iketani H, Nunome T, Kage T, Hirai M (2002) Isolation and characterization of microsatellite in Brassica rapa L. Theor Appl Genet 104:1092–1098CrossRefGoogle Scholar
  23. Suwabe K, Tsukazaki H, Iketani H, Hatakeyama K, Fujimura M, Nunome T, Fukuoka S, Matsumoto S, Hirai M (2003) Identification of two loci for resistance to clubroot (Plasmodiophora brassicae Woronin) in Brassica rapa L. Theor Appl Genet (in press)Google Scholar
  24. Toxopeus H, Janssen AMP (1975) Clubroot resistance in turnip II. The slurry screening method and clubroot races in the Netherlands. Euphytica 24:751–755Google Scholar
  25. Truco MJ, Hu J, Sadowski J, Quiros CF (1996) Inter- and intra-genomic homology of the Brassica genomes: implications for their origin and evolution. Theor Appl Genet 93:1225–1233CrossRefGoogle Scholar
  26. Voorrips RE, Jongerius MC, Kanne HJ (1997) Mapping of two genes for resistance to clubroot (Plasmodiophora brassicae) in a population of doubled haploid lines of Brassica oleracea by means of RFLP and AFLP markers. Theor Appl Genet 94:75–82CrossRefGoogle Scholar
  27. Williams PH (1966) A system for the determination of races of Plasmodiophora brassicae that infect cabbage and rutabaga. Phytopathology 56:624–626Google Scholar
  28. Wit F (1964) Inheritance of reaction to clubroot in turnips. Hortic Res 5:47–49Google Scholar
  29. Yoshikawa (1993) Studies on breeding of clubroot resistance in cole crops (in Japanese with English summary). Bull Natl Res Inst Veg Ornam Plants Tea Jpn Ser A 7:1-165Google Scholar

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • M. Hirai
    • 1
    Email author
  • T. Harada
    • 1
  • N. Kubo
    • 1
  • M. Tsukada
    • 2
  • K. Suwabe
    • 3
  • S. Matsumoto
    • 3
  1. 1.Faculty of AgricultureKyoto Prefectural UniversitySeika, Kyoto 619-0244Japan
  2. 2.Nagano Vegetable and Ornamental Crops Experiment StationMatsushiro, Nagano 381-1211Japan
  3. 3.National Institute of Vegetables and Tea Science (NIVTS)Japan

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