Theoretical and Applied Genetics

, Volume 108, Issue 2, pp 349–359 | Cite as

QTL and candidate genes phytoene synthase and ζ-carotene desaturase associated with the accumulation of carotenoids in maize

  • J. C. Wong
  • R. J. Lambert
  • E. T. Wurtzel
  • T. R. Rocheford
Article

Abstract

Carotenoids are a class of fat-soluble antioxidant vitamin compounds present in maize (Zea mays L.) that may provide health benefits to animals or humans. Four carotenoid compounds are predominant in maize grain: β-carotene, β-cryptoxanthin, zeaxanthin, and lutein. Although β-carotene has the highest pro-vitamin A activity, it is present in a relatively low concentration in maize kernels. We set out to identify quantitative trait loci (QTL) affecting carotenoid accumulation in maize kernels. Two sets of segregating families were evaluated—a set of F2:3 lines derived from a cross of W64a x A632, and their testcross progeny with AE335. Molecular markers were evaluated on the F2:3 lines and a genetic linkage map created. High-performance liquid chromatography was performed to measure β-carotene, β-cryptoxanthin, zeaxanthin, and lutein on both sets of materials. Composite interval mapping identified chromosome regions with QTL for one or more individual carotenoids in the per se and testcross progenies. Notably QTL in the per se population map to regions with candidate genes, yellow 1 and viviparous 9, which may be responsible for quantitative variation in carotenoids. The yellow 1 gene maps to chromosome six and is associated with phytoene synthase, the enzyme catalyzing the first dedicated step in the carotenoid biosynthetic pathway. The viviparous 9 gene maps to chromosome seven and is associated with ζ-carotene desaturase, an enzyme catalyzing an early step in the carotenoid biosynthetic pathway. If the QTL identified in this study are confirmed, particularly those associated with candidates genes, they could be used in an efficient marker-assisted selection program to facilitate increasing levels of carotenoids in maize grain.

Notes

Acknowledgements

This research was supported by a grant from the Bi-National Agricultural Research and Development Fund (BARD), and also supported by a University of Illinois Research Board Grant and the Agricultural Experiment Station. JCW was supported by the BARD grant, and by Pioneer Hi-Bred, Troyer/Darwin, and William and Nancy Ambrose Fellowships. We would also like to thank the following people for their support and assistance: Craig Anderson, Jerry Chandler, Janet Jackson, Jeremy Johnson, Venugopal Mikkilineni, Chandra Paul, Don Roberts, Jennifer Schultz, and Shane Zimmerman. We thank Martin Bohn for critical reading of the manuscript.

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • J. C. Wong
    • 1
    • 3
  • R. J. Lambert
    • 1
  • E. T. Wurtzel
    • 2
  • T. R. Rocheford
    • 1
  1. 1.Department of Crop SciencesUniversity of IllinoisUrbanaUSA
  2. 2.Department of Biological Sciences, Lehman College and The Graduate School and University CenterThe City University of New YorkWest BronxUSA
  3. 3.Horticulture and Crop Science DepartmentCalifornia Polytechnic State UniversitySan Luis ObispoUSA

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