Theoretical and Applied Genetics

, Volume 107, Issue 8, pp 1357–1361 | Cite as

The use of the S haplotype-specific F-box protein gene, SFB, as a molecular marker for S-haplotypes and self-compatibility in Japanese apricot (Prunus mume)

  • Hisayo Yamane
  • Koichiro Ushijima
  • Hidenori Sassa
  • Ryutaro Tao


Japanese apricot (Prunus mume) exhibits the S-RNase-based gametophytic self-incompatibility system as do other self-incompatible Prunus species. This report identifies the S haplotype-specific F-box protein gene (SFB), a candidate gene for pollen-S, of Japanese apricot, which leads to the development of a molecular typing system for S-haplotype in this fruit species. Both 5′- and 3′-RACE (rapid amplification of cDNA ends) were performed with SFB gene-specific oligonucleotide primers to clone Pm-SFB 1 and Pm-SFB 7 of 'Nanko (S 1 S 7 )'. As in the case of SFB of other Prunus species, Pm-SFB 1 and Pm-SFB 7 showed a high level of S-haplotype-specific sequence polymorphism and their expression was specific to pollen. Genomic DNA-blot analyses of 11 Japanese apricot cultivars with the Pm-SFB probes under low stringency conditions yielded RFLP bands specific to the S 1 - to S 8 -haplotypes as well as a self-compatible S f -haplotype. A practical usage of SFB as a molecular marker for S-haplotypes and self-compatibility in Japanese apricot is discussed.

Key words

F-box motif Gametophytic self-incompatibility Pollen determinant Prunus mume SFB S-RNase 



The authors gratefully acknowledge the gifts of plant material from Fukui Prefectural Horticultural Experiment Station (Fukui, Japan) and the National Institute of Fruit Tree Science (Tsukuba, Japan). This work was supported by a Grant-in-Aid (no. 13460014) for Scientific Research (B) to R.T. from the Japan Society for the Promotion of Science.


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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Hisayo Yamane
    • 1
  • Koichiro Ushijima
    • 1
  • Hidenori Sassa
    • 2
  • Ryutaro Tao
    • 1
  1. 1.Laboratory of Pomology, Graduate School of AgricultureKyoto UniversityKyotoJapan
  2. 2.Kihara Institute for Biological ResearchYokohama City UniversityYokohamaJapan

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