Theoretical and Applied Genetics

, Volume 107, Issue 8, pp 1357–1361 | Cite as

The use of the S haplotype-specific F-box protein gene, SFB, as a molecular marker for S-haplotypes and self-compatibility in Japanese apricot (Prunus mume)

  • Hisayo Yamane
  • Koichiro Ushijima
  • Hidenori Sassa
  • Ryutaro Tao

Abstract

Japanese apricot (Prunus mume) exhibits the S-RNase-based gametophytic self-incompatibility system as do other self-incompatible Prunus species. This report identifies the S haplotype-specific F-box protein gene (SFB), a candidate gene for pollen-S, of Japanese apricot, which leads to the development of a molecular typing system for S-haplotype in this fruit species. Both 5′- and 3′-RACE (rapid amplification of cDNA ends) were performed with SFB gene-specific oligonucleotide primers to clone Pm-SFB1 and Pm-SFB7 of 'Nanko (S1S7)'. As in the case of SFB of other Prunus species, Pm-SFB1 and Pm-SFB7 showed a high level of S-haplotype-specific sequence polymorphism and their expression was specific to pollen. Genomic DNA-blot analyses of 11 Japanese apricot cultivars with the Pm-SFB probes under low stringency conditions yielded RFLP bands specific to the S1- to S8-haplotypes as well as a self-compatible Sf-haplotype. A practical usage of SFB as a molecular marker for S-haplotypes and self-compatibility in Japanese apricot is discussed.

Key words

F-box motif Gametophytic self-incompatibility Pollen determinant Prunus mume SFB S-RNase 

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Hisayo Yamane
    • 1
  • Koichiro Ushijima
    • 1
  • Hidenori Sassa
    • 2
  • Ryutaro Tao
    • 1
  1. 1.Laboratory of Pomology, Graduate School of AgricultureKyoto UniversityKyotoJapan
  2. 2.Kihara Institute for Biological ResearchYokohama City UniversityYokohamaJapan

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