Theoretical and Applied Genetics

, Volume 107, Issue 7, pp 1195–1200 | Cite as

Oryzalin-induced chromosome doubling in Rosa and its effect on plant morphology and pollen viability

  • M. J. Kermani
  • V. Sarasan
  • A. V. RobertsEmail author
  • K. Yokoya
  • J. Wentworth
  • V. K. Sieber


Shoot tips of the diploid rose Thérèse Bugnet were treated in vitro to oryzalin at concentrations of 5 and 15 μM. Tetraploid shoots were obtained in highest frequencies (40%) after exposure to 5 μM oryzalin for 14 days. Thin (1 mm) nodal sections were treated with 5 μM oryzalin and the highest frequency of tetraploids (66%) was obtained after exposure for only 1 day. The shorter exposure times required to induce chromosome doubling in thin nodal sections is attributed to the more efficient delivery of oryzalin to the meristem. Tetraploids were obtained from four diploid roses and hexaploids from two triploid roses. Chromosome doubling was accompanied by increases in thickness and a darker green colouration of the leaves and, in all diploid to tetraploid and one triploid to hexaploid conversion, the breadth/length ratio of leaflets was significantly increased. Internodes were longer in tetraploids than diploids but significantly shorter in hexaploids than triploids. The number of petals per flower in the tetraploid form of Thérèse Bugnet was double that of the diploid. Significant increases in pollen viability accompanied chromosome doubling of all four diploids and one of the two triploids.


Colchicine Root Meristem Pollen Viability Chromosome Doubling Oryzalin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The authors acknowledge financial support from the Department of the Environment, Food and Rural Affairs, UK and David Austin Roses Ltd and thank Dr. Sara Walker for her help.


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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • M. J. Kermani
    • 1
  • V. Sarasan
    • 1
    • 2
  • A. V. Roberts
    • 1
    Email author
  • K. Yokoya
    • 1
    • 3
  • J. Wentworth
    • 1
    • 4
  • V. K. Sieber
    • 1
    • 5
  1. 1.School of Health and BioscienceUniversity of East LondonLondonUK
  2. 2.Micropropagation UnitRoyal Botanic GardensKewUK
  3. 3.Sir Alexander Flemming BuildingImperial College LondonLondonUK
  4. 4.Royal Commission on Environmental PollutionThe SanctuaryLondonUK
  5. 5.Medical Sciences Division, Department of Experimental PsychologyUniversity of OxfordOxfordUK

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