Theoretical and Applied Genetics

, Volume 107, Issue 7, pp 1201–1207 | Cite as

High variability and disomic segregation of microsatellites in the octoploid Fragaria virginiana Mill. (Rosaceae)

  • M. V. Ashley
  • J. A. Wilk
  • S. M. N. Styan
  • K. J. Craft
  • K. L. Jones
  • K. A. Feldheim
  • K. S. Lewers
  • T. L. Ashman
First Online:
Received:
Accepted:

Abstract

The objectives of the present study were to develop microsatellite markers for the wild strawberry, Fragaria virginiana, to evaluate segregation patterns of microsatellite alleles in this octoploid species, and assess genetic variability at microsatellite loci in a wild population. A genomic library was screened for microsatellite repeats and several PCR primers were designed and tested. We also tested the use of heterologous primers and found that F. virginiana primers amplified products in cultivated strawberry, Fragaria × ananassa Duch. and Fragaria chiloensis. Similarly, microsatellite loci developed from cultivated strawberry also successfully amplified F. virginiana loci. We investigated four microsatellite loci in detail, three developed from F. virginiana and one from cultivated strawberry. A survey of 100 individuals from a population of F. virginiana in Pennsylvania demonstrated high heterozygosities (He or gene diversity ranged from 0.80 to 0.88 per locus) and allelic diversity (12–17 alleles per locus), but individual plants had no more than two alleles per locus. Segregation patterns in parents and progeny of two controlled crosses at these four loci were consistent with disomic Mendelian inheritance. Together these findings suggest that the genome of F. virginiana is "highly diploidized" and at least a subset of microsatellite loci can be treated as codominant, diploid markers. Significant heterozygote deficiencies were found at three of the four loci for hermaphroditic individuals but for only one locus among females in this gynodioecious species.

Keywords

Fragaria virginiana Wild strawberry Microsatellites Polyploid Octoploid Fragaria × ananassa 
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Notes

Acknowledgements

The authors thank E. York, S. Warnagiris, J. Fessler, C. Evenovski, K. Salazar and S. Garrett for greenhouse, laboratory and other assistance. This work was supported by the National Science Foundation (DEB 9903802 and 9904115 to T.-L.A. and M.V.A, respectively). This work was conducted in part while M.V.A. was a Sabbatical Fellow at the National Center for Ecological Analysis and Synthesis, a Center funded by the National Science Foundation, the University of California, and the Santa Barbara Campus. This is contribution 129 to the Pymatuning Laboratory of Ecology.

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • M. V. Ashley
    • 1
  • J. A. Wilk
    • 1
  • S. M. N. Styan
    • 2
    • 4
  • K. J. Craft
    • 1
  • K. L. Jones
    • 1
  • K. A. Feldheim
    • 1
  • K. S. Lewers
    • 2
  • T. L. Ashman
    • 3
  1. 1.Department of Biological SciencesUniversity of Illinois at ChicagoChicagoUSA
  2. 2.USDA-ARS Fruit LabBld 010A, BARC-WBeltsvilleUSA
  3. 3.Department of Biological SciencesUniversity of PittsburghPittsburghUSA
  4. 4.Pioneer Hi-Bred International Incorporated. A DuPont CompanyWaimeaUSA

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