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Mapping osmotic adjustment in an advanced back-cross inbred population of rice

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Abstract.

Osmotic adjustment is one of several characters putatively associated with drought tolerance in rice. Indica cultivars are known to have a greater capacity for osmotic adjustment than japonica cultivars. We developed an advanced back-cross population using an indica donor, IR62266-42-6-2, to introgress osmotic adjustment into an elite japonica cultivar, IR60080-46A. One hundred and fifty BC3F3 families were genotyped using microsatellites and RFLP markers, and a few candidate genes. We evaluated osmotic adjustment in these lines under greenhouse conditions using the re-hydration technique. Using the composite interval mapping technique, we detected 14 QTLs located on chromosomes 1, 2, 3, 4, 5, 7, 8 and 10 that together explained 58% of the phenotypic variability. Most, but not all, of the alleles with positive effects came from the donor parent. On chromosome 8, two QTLs were associated in repulsion. The QTL locations were in good agreement with previous studies on this trait on rice and in other cereals. Some BC3F3 lines carried the favorable alleles at the two markers flanking up to four QTLs. Intercrossing these lines followed by marker-aided selection in their progenies will be necessary to recover lines with levels of osmotic adjustment equal to the donor parent. The advanced back-cross strategy appeared to be an appropriate method to accelerate the process of introgressing interesting traits into elite material.

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Acknowledgements.

This work was supported by the Rockefeller Foundation and by USAID through their grants to the International Rice Research Institute, The Philippines.

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Author notes

  1. S. Robin

    Present address: Tamil Nadu Agricultural University, Coimbatore 641 003, Tamil Nadu, India,

  2. B. Courtois

    Present address: Centre de Coopération Internationale en Recherche Agronomique pour le Développement, TA 4003 Avenue Agropolis, 34398 Montpellier Cedex 5, France,

Authors and Affiliations

  1. International Rice Research Institute, MCPO Box 3127, 1271 Makati City, The Philippines

    S. Robin, B. Courtois, R. Lafitte, S. Carandang, S. Lanceras, M. Amante & Z. Li

  2. Department of Agronomy, University of Missouri-Columbia, MO 65211, USA

    M. S. Pathan & H. T. Nguyen

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  1. S. Robin
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Correspondence to B. Courtois.

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Communicated by Q. Zhang

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Robin, S., Pathan, M.S., Courtois, B. et al. Mapping osmotic adjustment in an advanced back-cross inbred population of rice. Theor Appl Genet 107, 1288–1296 (2003). https://doi.org/10.1007/s00122-003-1360-7

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  • DOI: https://doi.org/10.1007/s00122-003-1360-7

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