Theoretical and Applied Genetics

, Volume 107, Issue 6, pp 997–1002 | Cite as

Identification of two loci for resistance to clubroot (Plasmodiophora brassicae Woronin) in Brassica rapa L.

  • K. Suwabe
  • H. Tsukazaki
  • H. Iketani
  • K. Hatakeyama
  • M. Fujimura
  • T. Nunome
  • H. Fukuoka
  • S. MatsumotoEmail author
  • M. Hirai


In an analysis of 114 F2 individuals from a cross between clubroot-resistant and susceptible lines of Brassica rapa L., 'G004' and 'Hakusai Chukanbohon Nou 7' (A9709), respectively, we identified two loci, Crr1 and Crr2, for clubroot (caused by Plasmodiophora brassicae Woronin) resistance. Each locus segregated independently among the F2 population, indicating that the loci reside on a different region of chromosomes or on different chromosomes. Genetic analysis showed that each locus had little effect on clubroot resistance by itself, indicating that these two loci are complementary for clubroot resistance. The resistance to clubroot was much stronger when both loci were homozygous for resistant alleles than when they were heterozygous. These results indicate that clubroot resistance in B. rapa is under oligogenic control and at least two loci are necessary for resistance.


Brassica rapa Clubroot Microsatellites (SSRs) Plasmodiophora brassicae Disease resistance 



We thank Prof. Dr. Y. Kowyama, Dr. K. Kakeda and Dr. T. Tsuchiya of Mie University for their valuable advice. We are grateful to Ms. K. Tanaka and Ms. H. Maeda for their technical assistance. This work was supported by the Cooperative System for Supporting Priority Research of the Japan Science and Technology Corporation and by grant from the Ministry of Agriculture, Forestry and Fisheries of Japan (Rice Genome Project DM-2105).


  1. Bell CJ, Ecker JR (1994) Assignment of 30 microsatellite loci to the linkage map of Arabidopsis. Genomics 19:137–144CrossRefPubMedGoogle Scholar
  2. Bittner-Eddy PD, Crute IR, Holub EB, Beynon JL (2000) RPP13 is a simple locus in Arabidopsis thaliana for alleles that specify downy mildew resistance to different avirulence determinants in Peronospora parasitica. Plant J 21:177–188CrossRefPubMedGoogle Scholar
  3. Botella MA, Coleman MJ, Hughes DE, Nishimura MT, Jones JDG, Somerville SC (1997) Map positions of 47 Arabidopsis sequences with sequence similarity to disease-resistant genes. Plant J 12:1197–1211PubMedGoogle Scholar
  4. Buczacki ST, Toxopeus H, Mattusch P, Johnston TD, Dixon GR, Hobolth LA (1975) Study of physiologic specialization in Plasmodiophora brassicae: proposals for rationalization through an international approach. Trans Br Mycol Soc 65:295–303Google Scholar
  5. Chiang BY, Chiang MS, Grant WF, Crete R (1980) Transfer of resistance to race 2 of Plasmodiophora brassicae from Brassica napus to cabbage (B. oleracea spp. capitata). IV. A resistant 18-chromosome B1 plant and its B2 progenies. Euphytica 29:47–55Google Scholar
  6. Crute IR, Pink DAC (1996) Genetic and utilization of pathogen resistance in plants. Plant Cell 8:1747–1755Google Scholar
  7. Crute IR, Gray AR, Crisp P, Buczacki ST (1980) Variation in Plasmodiophora brassicae and resistance to clubroot disease in brassicas and allied crops – a critical review. Plant Breed Abstr 50:91–104Google Scholar
  8. Dib C, Faure S, Fizames C, Samson D, Drouot N, Vignal A (1996) A comprehensive genetic map of the human genome based on 5,264 microsatellites. Nature 380:152–154PubMedGoogle Scholar
  9. Dietrich WF, Miller J, Steen R, Merchant MA, Damron-Boles D, Husain Z (1996) A comprehensive genetic map of the mouse genome. Nature 380:149–152PubMedGoogle Scholar
  10. Figdore SS, Ferreira ME, Slocum MK, Williams PH (1993) Association of RFLP markers with trait loci affecting clubroot resistance and morphological characters in Brassica oleracea L. Euphytica 69:33–44Google Scholar
  11. Ford CM, Paltridge NG, Rathjen JP, Moritz RL, Simpson RJ, Symons RH (1998) Rapid and informative assays for Yd2, the barley yellow dwarf virus resistance gene, based on the nucleotide sequence of a closely linked gene. Mol Breed 4:23–31CrossRefGoogle Scholar
  12. Fuchs H, Sacristán MD (1996) Identification of a gene in Arabidopsis thaliana controlling resistance to clubroot (Plasmodiophora brassicae) and characterization of the resistance response. Mol Plant-Microbe Interact 9:91–97Google Scholar
  13. Grandclément C, Thomas G (1996) Detection and analysis of QTLs based on RAPD markers for polygenic resistance to Plasmodiophora brassicae Woron in Brassica oleracea L. Theor Appl Genet 93:86–90CrossRefGoogle Scholar
  14. Gupta PK, Varshney RK (2000) The development and use of microsatellite markers for genetic analysis and plant breeding with emphasis on bread wheat. Euphytica 113:163–185CrossRefGoogle Scholar
  15. Hancock JM (1995) The contribution of slippage-like processes to genome evolution. J Mol Evol 41:1038–1047PubMedGoogle Scholar
  16. Hayashi T, Ukai Y (1999) Method of QTL mapping in an F2 population using phenotypic means of F3 lines. Breed Sci 49:105–114Google Scholar
  17. Jones DR, Ingram DS, Dixon GR (1982) Characterization of isolates derived from single resting spores of Plasmodiophora brassicae and studies of their interaction. Plant Pathol 31:239–246Google Scholar
  18. Kandemir N, Jones BL, Wesenberg DM, Ullrich SE, Kleinhofs A (2000) Marker-assisted analysis of three grain yield QTLs in barley (Hordeum vulgare L.) using near-isogenic lines. Mol Breed 6:157–167CrossRefGoogle Scholar
  19. Karling JS (1968) The Plasmodiophorales. Hafner Publishing Company, New YorkGoogle Scholar
  20. Kikuchi M, Ajisaka H, Kuginuki Y, Hirai M (1999) Conversion of RAPD markers for a clubroot resistance gene of Brassica rapa into sequence-tagged sites (STSs). Breed Sci 49:83–88Google Scholar
  21. Kresovich S, Szewc-McFadden AK, Bliek SM, McFerson JR (1995) Abundance and characterization of simple-sequence repeats (SSRs) isolated from a size-fractionated genomic library of Brasica napus L. (rapessed). Theor Appl Genet 91:206–211Google Scholar
  22. Kuginuki Y, Ajisaka H, Yui M, Yoshikawa H, Hida K, Hirai M (1997) RAPD markers linked to a clubroot-resistance locus in Brassca rapa L. Euphytica 98:149–154CrossRefGoogle Scholar
  23. Kuginuki Y, Yoshikawa H, Hirai M (1999) Variation in virulence of Plasmodiophora brassicae in Japan tested with clubroot-resistant cultivars of Chinese cabbage (Brassica rapa L. spp. pekinensis). Eur J Plant Pathol 105:327–332CrossRefGoogle Scholar
  24. Landry BS, Hubert N, Crête R, Chang MS, Lincoln SE, Etoh T (1992) A genetic map of Brassica oleracea based on RFLP markers detected with expressed DNA sequences and mapping of resistance genes to race 2 of Plasmodiophora brassicae (Woronin). Genome 35:409–419Google Scholar
  25. Morgante M, Olivieri AM (1993) PCR-amplified microsatellites as markers in plant genetics. Plant J 3:175–182PubMedGoogle Scholar
  26. Parker GD, Langridge P (2000) Development of STS marker linked to a major locus controlling flower color in wheat (Triticum aestivum L.). Mol Breed 6:169–174Google Scholar
  27. Speulman E, Bouchez D, Holub EB, Beynon JL (1998) Disease resistance gene homologs correlate with disease resistance loci of Arabidopsis thaliana. Plant J 14:467–474PubMedGoogle Scholar
  28. Suwabe K, Iketani H, Nunome T, Kage T, Hirai M (2002) Isolation and characterization of microsatellites in Brassica rapa L. Theor Appl Genet 104:1092–1098CrossRefGoogle Scholar
  29. Tautz D, Renz M (1984) Simple sequences are ubiquitous repetitive components of eukaryotic genomes. Nucleic Acids Res 25:4127–4138Google Scholar
  30. Uzunova MI, Ecke W (1999) Abundance, polymorphism and genetic mapping of microsatellites in oilseed rape (Brassica napus L.). Plant Breed 118:323–326CrossRefGoogle Scholar
  31. Voorrips RE (1995) Plasmodiophora brassicae: aspects of pathogenesis and resistance in Brassica oleracea. Euphytica 83:139–146Google Scholar
  32. Voorrips RE, Jongerius MC, Kanne HJ (1997) Mapping of two genes for resistance to clubroot (Plasmodiophora brassicae) in a population of doubled-haploid lines of Brassica oleracea by means of RFLP and AFLP markers. Theor Appl Genet 94:75–82CrossRefGoogle Scholar
  33. Westman AL, Kresovich S (1999) Simple sequence repeat (SSR)-based marker variation in Brassica nigra genebank accessions and weed populations. Euphytica 109:85–92CrossRefGoogle Scholar
  34. Williams PH (1966) A system for the determination of races of Plasmodiophora brassicae that infect cabbage and rutabaga. Phytopathology 56:624–626Google Scholar
  35. Yoshikawa H (1981) Breeding for clubroot resistance in Chinese cabbage. In: Talekar NS, Griggs TD (eds) Chinese cabbage. Proc 1st Int Symp, Tsukuba, Japan, pp 405–413Google Scholar

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • K. Suwabe
    • 1
  • H. Tsukazaki
    • 1
  • H. Iketani
    • 2
  • K. Hatakeyama
    • 1
  • M. Fujimura
    • 1
  • T. Nunome
    • 1
  • H. Fukuoka
    • 1
  • S. Matsumoto
    • 1
    Email author
  • M. Hirai
    • 1
    • 3
  1. 1.National Institute of Vegetable and Tea Science (NIVTS), 360 Kusawa, Ano, Age, Mie 514-2392, Japan
  2. 2.National Agricultural Research Organization, 3-1-1 Kannon-dai, Tsukuba, Ibaraki 305-8517, Japan
  3. 3.Faculty of Agriculture, University of Kyoto prefecture, Kitainaazuma, Seika, Kyoto 619-0244, Japan

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