Molecular cytogenetic analysis of genome structure in Lupinus angustifolius and Lupinus cosentinii

Inga Hajdera; Dorota Siwinska; Robert Hasterok; Jolanta Maluszynska

doi:10.1007/s00122-003-1303-3

Theoretical and Applied Genetics

October 2003, Volume 107, Issue 6, pp 988–996

Molecular cytogenetic analysis of genome structure in Lupinus angustifolius and Lupinus cosentinii

Authors
Authors and affiliations

Inga Hajdera
Dorota Siwinska
Robert HasterokEmail author
Jolanta Maluszynska

Article

First Online:: 02 October 2003

Received:: 28 November 2002
Accepted:: 20 January 2003

DOI: 10.1007/s00122-003-1303-3

Cite this article as:: Hajdera, I., Siwinska, D., Hasterok, R. et al. Theor Appl Genet (2003) 107: 988. doi:10.1007/s00122-003-1303-3

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Abstract

Molecular cytogenetic analysis of Lupinus angustifolius and Lupinus cosentinii was performed using flow cytometry, fluorescence in situ hybridisation (FISH) and differential chromosome staining. Genome size was determined as 2.07 pg for L. angustifolius and 1.54 pg for L. cosentinii. Analysis of nuclear DNA amount in cells during plant development has shown endopolyploidisation in different organs. The highest level of endopolyploidy was in cotyledons and reached 32C in L. angustifolius and 64C in L. cosentinii. Both of the investigated Lupinus species belong to the polysomatic type of plants. Double FISH with rDNA probes provided chromosomal landmarks for 10 out of 40 chromosomes for L. angustifolius and 8 out of 32 chromosomes for L. cosentinii. In L. angustifolius, the number and localisation of 25S rDNA hybridisation signals precisely corresponded to the chromomycin A3 (CMA⁺) bands, while in L. cosentinii both 25S and 5S rDNA loci overlapped with CMA⁺ bands. Silver staining revealed that only 45S rRNA genes located in secondary constriction regions were transcriptionally active. FISH with Arabidopsis-type telomeric arrays revealed the presence of signals at termini of all chromosomes. Despite the application of different DNA probes for FISH and different chromosome staining, a relatively small proportion of chromosomes in the Lupinus karyotypes can be distinguished. Identification of all chromosomes requires the use of more chromosome-specific markers.

Keywords

FISH Flow cytometry Lupinus rDNA Telomere

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Authors and Affiliations

Inga Hajdera
- 1
Dorota Siwinska
- 1
Robert Hasterok
- 1
Email author
Jolanta Maluszynska
- 1

1.Department of Plant Anatomy and Cytology, University of Silesia, Jagiellonska 28, 40-032 Katowice, Poland

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Molecular cytogenetic analysis of genome structure in Lupinus angustifolius and Lupinus cosentinii

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