Theoretical and Applied Genetics

, Volume 106, Issue 6, pp 1041–1047 | Cite as

Detection of Fusarium head blight resistance QTL in a wheat population using bulked segregant analysis

  •  X. Shen
  •  M. Zhou
  •  W. Lu
  •  H. Ohm

Abstract.

A population of 218 recombinant inbred lines (RILs) was developed from the cross of two wheat (Triticum aestivum L.) cultivars, 'Ning 894037' and 'Alondra'. Ning 894037 has resistance to Fusarium head blight (FHB) and Alondra is moderately susceptible. Response of the RILs and their parental lines to FHB infection was evaluated with point inoculation in four experiments both in greenhouse and in field conditions. Distribution of disease severity in the population is continuous, indicating quantitative inheritance of resistance to FHB. Bulked segregant analysis and QTL mapping based on simple sequence repeat (SSR) markers revealed three chromosome regions that are responsible for FHB resistance. A chromosome region on 3BS accounted for 42.5% of the phenotypic variation for FHB resistance. Additional QTLs were located on chromosomes 2D and 6B. These three QTLs jointly accounted for 51.6% of the phenotypic variation. SSR markers linked to the QTLs influencing resistance to FHB have potential for use in breeding programs.

Wheat Fusarium head blight (FHB) Quantitative trait loci (QTL) Simple sequence repeat (SSR) 

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  •  X. Shen
    • 1
  •  M. Zhou
    • 2
  •  W. Lu
    • 2
  •  H. Ohm
    • 1
  1. 1.Department of Agronomy, 1150 Lilly Hall, Purdue University, West Lafayette, IN 47907, USA
  2. 2.Institute of Genetics and Physiology, Jiangsu Academy of Agricultural Science, 50 Zhongling Street, Nanjing, Jiangsu, P.R. China

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