Theoretical and Applied Genetics

, Volume 106, Issue 4, pp 727–734 | Cite as

Development of isothiocyanate-enriched broccoli, and its enhanced ability to induce phase 2 detoxification enzymes in mammalian cells

  •  R. Mithen
  •  K. Faulkner
  •  R. Magrath
  •  P. Rose
  •  G. Williamson
  •  J. Marquez

Abstract.

Broccoli florets contain low levels of 3-methylsuphinylpropyl and 4-methylsulphinylbutyl glucosinolates. Following tissue disruption, these glucosinolates are hydrolysed to the corresponding isothiocyanates (ITCs), which have been associated with anticarcinogenic activity through a number of physiological mechanisms including the induction of phase II detoxification enzymes and apoptosis. In this paper, we describe the development of ITC-enriched broccoli through the introgression of three small segments of the genome of Brassica villosa, a wild relative of broccoli, each containing a quantitative trait locus (QTL), into a broccoli genetic background, via marker-assisted selection and analysis of glucosinolates in the florets of backcross populations. Epistatic and heterotic effects of these QTLs are described. The ITC-enriched broccoli had 80-times the ability to induce quinone reductase (a standard assay of phase II induction potential) when compared to standard commercial broccoli, due both to an increase in the precursor glucosinolates and a greater conversion of these into ITCs.

Isothiocyanates Glucosinolates Nitriles Broccoli Anticarcinogenesis Phase 2 enzymes Marker-assisted selection 

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  •  R. Mithen
    • 1
  •  K. Faulkner
    • 2
  •  R. Magrath
    • 2
  •  P. Rose
    • 2
  •  G. Williamson
    • 3
  •  J. Marquez
    • 1
  1. 1.School of Biosciences, University of Nottingham, Sutton Bonington, LE12 5RD, UK
  2. 2.John Innes Centre, Colney Lane, Norwich, NR4 7UH, UK
  3. 3.Institute of Food Research, Colney Lane, Norwich NR4 7UA, UK

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