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Theoretical and Applied Genetics

, Volume 106, Issue 4, pp 688–695 | Cite as

Reproduction and cytogenetic characterization of interspecific hybrids derived from Cucumis hystrix Chakr. × Cucumis sativus L.

  •  J. Chen
  •  J. Staub
  •  Ch. Qian
  •  J. Jiang
  •  X. Luo
  •  F. Zhuang

Abstract.

Interspecific hybrids between Cucumis hystrix Chakr. (2n = 2x = 24) and Cucumis sativus L. (2n = 2x = 14) were produced by means of F1 (2n = 19) embryo rescue and subsequent chromosome doubling. The hybridity was confirmed by genomic in situ hybridization (GISH) and chromosome analysis. The amphidiploid (2n = 38) was self-pollinated and backcrossed to cucumber resulting in lines with improved crossability to C. sativus. Examination of shape, stainability, and germination rate of pollen grains and yield as a function of mature fruit set per ten pollinated flowers indicated a tendency for increased fertility in BC1S1 progeny when compared to F1 and amphidiploid offspring. Cytogenetic characterization of F1 and amphidiploid progeny was performed. Generally normal meioses produced viable pollen grains, and fertilization resulted in partial fertility restoration in amphidiploid progeny. Chromosome anomalies such as "frying-pan trivalent", chromosome lagging and spindle mis-orientation were also observed. In most of the PMCs of the F1 diploid hybrid progeny, 19 univalents were observed at diakinesis and MI. In the amphidiploid, more than 90% of the configurations at MI consisted of the predicted 19 bivalents and less than 5% contained multivalents [trivalents (2.3%) + quadrivalents (0.3%)], suggesting the presence of preferential pairing, and a distinctive parental genome as well. The chiasmata observed between homoeologous chromosomes further demonstrated the introgression of the C. hystrix genome into that of C. sativus.

Interspecific hybridization Amphidiploid GISH Chromosome pairing 

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  •  J. Chen
    • 1
  •  J. Staub
    • 2
  •  Ch. Qian
    • 1
  •  J. Jiang
    • 3
  •  X. Luo
    • 1
  •  F. Zhuang
    • 1
  1. 1.State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095 China
  2. 2.U.S. Department of Agriculture, Agricultural Research Service
  3. 3.Department of Horticulture, University of Wisconsin-Madison, 1575 Linden Drive, Madison WI 53706 USA

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