Theoretical and Applied Genetics

, Volume 106, Issue 1, pp 173–180 | Cite as

Assessment of population genetic structure in common wild rice Oryza rufipogon Griff. using microsatellite and allozyme markers

  •  L-z. Gao
  •  B. Schaal
  •  C-h. Zhang
  •  J-z. Jia
  •  Y-s. Dong

Abstract.

The genetic structure of five natural populations of common wild rice Oryza rufipogon Griff. from China, was investigated with 21 microsatellite loci and compared to estimates of genetic diversity and genetic differentiation detected by 22 allozyme loci. Microsatellite loci, as expected, have much higher levels of genetic diversity (mean values of A = 3.1, P = 73.3%, Ho = 0.358 and He = 0.345) than allozyme loci (mean values of A = 1.2, P = 12.7%, Ho = 0.020 and He = 0.030). Genetic differentiation detected by microsatellite loci (FST = 0.468, mean I = 0.472) was higher than that for allozyme loci (FST =0.388, mean I = 0.976). However, microsatellite markers showed less deviation from Hardy-Weinberg expectation (Wright's inbreeding coefficient FIS = –0.069) than do allozymes (FIS = 0.337). These results suggest that microsatellite markers are powerful high-resolution tools for the accurate assessment of important parameters in population biology and conservation genetics of O. rufipogon, and offer advantages over allozyme markers.

China Allozyme variation Microsatellite variation Oryza rufipogon Population genetic structure 

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  •  L-z. Gao
    • 1
  •  B. Schaal
    • 2
  •  C-h. Zhang
    • 1
  •  J-z. Jia
    • 1
  •  Y-s. Dong
    • 1
  1. 1.Key Laboratory of Crop Germplasm and Biotechnology, Ministry of Agriculture of China, Institute of Crop Germplasm Resources, Chinese Academy of Agricultural Sciences, Beijing 100081, P. R. China
  2. 2.Department of Biology, Campus Box 1137, Washington University, St. Louis, MO 63130-4899, USA
  3. 3.Present address: Department of Ecology and Evolutionary Biology, University of Michigan, Natural Science Building, 830 North University Avenue, Ann Arbor, MI 48109, USA

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