Theoretical and Applied Genetics

, Volume 105, Issue 6–7, pp 1000–1009 | Cite as

AFLP maps of Petunia hybrida: building maps when markers cluster

  •  J. Strommer
  •  J. Peters
  •  J. Zethof
  •  P. de Keukeleire
  •  T. Gerats

Abstract.

AFLP mapping in Petunia hybrida was undertaken with the intention of building a high-density genetic map suitable for applications such as map-based gene cloning. In total five maps were constructed from two mapping populations, with placement of more than 800 markers. Despite the large number of markers the resulting map is roughly ten-fold smaller than those of other plant species, including the closely related tomato. Low levels of recombination are reflected in clusters of tightly linked markers, both AFLPs and RFLPs, in all the maps. Clustering patterns vary between mapping populations, however, such that loci tightly linked in one population may be separable in another. Combined with earlier reports of aberrant meiotic pairing and recombination, our results suggest that, for species like petunia, map-based cloning may be more complex than in model species such as arabidopsis and tomato.

AFLP Petunia hybrida Genetic map Marker clusters Recombination 

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Copyright information

© Springer 2002

Authors and Affiliations

  •  J. Strommer
    • 1
  •  J. Peters
    • 2
  •  J. Zethof
    • 2
  •  P. de Keukeleire
    • 2
  •  T. Gerats
    • 2
  1. 1.Department of Plant Agriculture and Department of Molecular Biology and Genetics, University of Guelph, Guelph, Ontario N1H 4W6, Canada
  2. 2.Department of Plant Genetics, Universiteit Gent/VIB, K.L Ledeganckstraat 35, B-9000 Gent, Belgium
  3. 3.Present addresses: Department of Plant Genetics, Universiteit van Nijmegen, Toernooiveld 1, 6525 ED, Nijmegen, The Netherlands

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