Theoretical and Applied Genetics

, Volume 105, Issue 6–7, pp 1087–1096

A genetic linkage map of willow (Salix viminalis) based on AFLP and microsatellite markers

  •  S. Hanley
  •  J. Barker
  •  J. Van Ooijen
  •  C. Aldam
  •  S. Harris
  •  I. Åhman
  •  S. Larsson
  •  A. Karp

Abstract.

The genus Salix (willow) contains a number of species of great value as biomass crops. Efforts to breed varieties with improved biomass yields and resistances to pests and diseases are limited by the lack of knowledge on the genetic basis of the traits. We have used AFLP and microsatellite markers to construct a genetic map of willow from a full-sib cross of the diploid species Salix viminalis (2n = 38). In accordance with a double pseudo-testcross approach, separate parental maps were constructed and merged to produce a consensus map comprising 291 AFLP and 39 willow microsatellite markers. Nineteen poplar microsatellites were also tested in willow. Five of these amplified loci, of which two were mapped. Linkage groups of the consensus map that could be identified in the parental maps are presented here and spanned 1,256.5 cM with an average interval between markers of 4.4 cM.

Willow Salix Biomass AFLP SSR Genetic map 

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Copyright information

© Springer 2002

Authors and Affiliations

  •  S. Hanley
    • 1
  •  J. Barker
    • 1
  •  J. Van Ooijen
    • 2
  •  C. Aldam
    • 1
  •  S. Harris
    • 1
  •  I. Åhman
    • 3
  •  S. Larsson
    • 3
  •  A. Karp
    • 1
  1. 1.IACR-Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, Long Ashton, Bristol, BS41 9AF, UK
  2. 2.Plant Research International, P.O. Box 16, 6700 AA Wageningen, The Netherlands
  3. 3.Svalöf Weibull AB, SE-268 81, Svalöv, Sweden

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