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Theoretical and Applied Genetics

, Volume 104, Issue 4, pp 571–576 | Cite as

Genetic analysis of Vrn-B1 for vernalization requirement by using linked dCAPS markers in bread wheat (Triticum aestivum L.)

  • K. Iwaki
  • J. Nishida
  • T. Yanagisawa
  • H. Yoshida
  • K. Kato

Abstract 

To identify a molecular marker closely linked to Vrn-B1, the Vrn-1 ortholog on chromosome 5B, sequence polymorphism at four orthologous RFLP loci closely linked to the Vrn-1 gene family was analyzed by using near-isogenic lines of ”Triple Dirk.” At Xwg644, a RFLP locus, three types of nucleotide sequence differing by the number of (TG) repeats, two or three times, and base changes were detected. A (TG)3-type sequence proved to be specific to chromosome 5B by nulli-tetrasomic analysis, and substitution of single nucleotide (C/T) was detected between TD(B) carrying the former Vrn2 allele and TD(C) carrying the vrn2 allele. A mismatch primer was designed for dCAPS analysis of this single nucleotide polymorphism (SNP). Polymorphism was successfully detected between two NILs, through nested PCR by using a (TG)3-specific primer (1st) and a dCAPS primer (2nd) followed by a NsiI digest. The analysis of a BF2 population [(TD(B)//TD(C)] revealed the close linkage (1.7 cM) between WG644–5B and Vrn2. It was therefore concluded that the former Vrn2 locus is located on chromosome 5B and equivalent to Vrn-B1.

Keywords dCAPS Orthologous gene Synteny Vernalization requirement Wheat 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2002

Authors and Affiliations

  • K. Iwaki
    • 1
  • J. Nishida
    • 1
  • T. Yanagisawa
    • 2
  • H. Yoshida
    • 2
  • K. Kato
    • 1
  1. 1.Faculty of Agriculture, Okayama University, 1-1-1 Tsushima-Naka, Okayama 700-8530, Japan e-mail: kenkato@cc.okayama-u.ac.jp, Fax. +81–86-251-8388, Present address: K. Iwaki, Ohmi Research Center, Flower Division, Suntory Limited, Youkaichi 523-0063, JapanJP
  2. 2.National Agriculture Research Center, 3–1-1 Kannondai, Tsukuba, Ibaraki 305-0856, JapanJP

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