Unrelated secondary reproductives in the neotropical termite Silvestritermes euamignathus (Isoptera: Termitidae)
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A termite colony is usually founded by a pair of alates, the primary reproductives, which produce all the nestmates. In some species, secondary reproductives appear to either replace the primaries or supplement colony reproduction. In termites, secondary reproductives are generally ergatoids derived from workers or nymphoids derived from nymphs. Silvestritermes euamignathus is a termite species that forms multiple nymphoid reproductives, and to date it was hypothesized that these secondary reproductives were the progeny of the primary founding reproductives. We developed markers for 12 microsatellite loci and used COI mitochondrial DNA (mtDNA) to genotype 59 nymphoid neotenics found in a colony of S. euamignathus to test this hypothesis. Our results showed that nymphoids of S. euamignathus are not all siblings. The microsatellite analysis suggests that the secondary reproductives derived from a minimum of four different pairs of reproductives belonging to at least two different matrilines. This is the first record of non-sibling secondary reproductives occupying the same nest in a higher termite. These unrelated reproductives might be the result of either pleometrotic colony foundation or colony fusion.
KeywordsMicrosatellites mtDNA COI Genotyping Neotenics
The authors thank FAPESP (Process No. 2012/23898-0, 2014/00720-6 and 2014/25857-4) and CNPq (Process No. 305539/2014-0) for financial support. The authors also thank the four anonymous reviewers for their critical contributions.
- Araujo RL (1958) Contribuição à biogeografia dos térmitas de São Paulo, Brasil (Insecta, Isoptera). Arq Inst Biol 25:185–217Google Scholar
- Costa-Leonardo AM, Barsotti RC, Soares HX (1996) Multiple nymphoid reproductives in the nests of the neotropical termite, Armitermes euamignathus (Isoptera, Termitidae, Nasutitermitinae). Sociobiology 28(2):197–205Google Scholar
- Goudet J (2002) FSTAT, a program to estimate and test gene diversities and fixation indicesGoogle Scholar
- Guaraldo AC, Costa-Leonardo AM (2009) Preliminary fusion testing between whole young colonies of Coptotermes gestroi (Isoptera: Rhinotermitidae). Sociobiology 53:767–774Google Scholar
- Hartke TR, Rosengaus RB (2013) Costs of pleometrosis in a polygamous termite. Proc R Soc Lond B Biol Sci 280:20122563Google Scholar
- Mathews AG (1977) Studies on termites from the Mato Grosso state, Brazil. Academia Brasileira de Ciências, Rio de Janeiro, p 267Google Scholar
- Miller LR (1969) Caste differentiation in the lower termites. In: Biology of Termites. Krishna K, Weesner FM (eds) pp. 283-310Google Scholar
- Myles TG (1999) Review of secondary reproduction in termites (Insecta: Isoptera) with comments on its role in termite ecology and social evolution. Sociobiology 33:1–91Google Scholar
- Noirot C (1969) Formation of castes in higher termites. In: Biology of Termites. Krishna K, Weesner FM (eds) pp. 311-350Google Scholar
- Vargo EL, Husseneder C (2011) Genetic structure of termite colonies and populations. In: Biology of Termites: a modern synthesis. Bignell DE, Roisin Y, Lo N (eds) pp. 321-347Google Scholar