The Science of Nature

, 102:19 | Cite as

Non-target effects of clothianidin on monarch butterflies

Short Communication

Abstract

Monarch butterflies (Danaus plexippus) frequently consume milkweed in and near agroecosystems and consequently may be exposed to pesticides like neonicotinoids. We conducted a dose response study to determine lethal and sublethal doses of clothianidin using a 36-h exposure scenario. We then quantified clothianidin levels found in milkweed leaves adjacent to maize fields. Toxicity assays revealed LC10, LC50, and LC90 values of 7.72, 15.63, and 30.70 ppb, respectively. Sublethal effects (larval size) were observed at 1 ppb. Contaminated milkweed plants had an average of 1.14 ± 0.10 ppb clothianidin, with a maximum of 4 ppb in a single plant. This research suggests that clothianidin could function as a stressor to monarch populations.

Keywords

Asclepias Danaus plexippus Neonicotinoid Non-target Seed treatment 

References

  1. Altizer SM, Oberhauser KS, Brower LP (2000) Association between host migration and the prevalence of a protozoan parasite in natural populations of adult monarch butterflies. Ecol Entomol 25:125–139CrossRefGoogle Scholar
  2. Brower LP, Zubieta RR, Ramirez MI, Slayback DA, Taylor OR, Williams EH (2012) Decline of monarch butterflies overwintering in Mexico: is the migratory phenomenon at risk? Insect Conserv Diver 5:95–100CrossRefGoogle Scholar
  3. Dively GP, Rose R, Sears MK, Hellmich RL, Stanley-Horn DE, Calvin DD, Russo JM, Anderson PL (2004) Effects on monarch butterfly larvae (Lepidoptera: Danaidae) after continuous exposure to Cry1Ab-expressing corn during anthesis. Environ Entomol 33:1116–1125CrossRefGoogle Scholar
  4. Goulson D (2013) An overview of the environmental risks posed by neonicotinoid insecticides. J Appl Ecol 50:977–987CrossRefGoogle Scholar
  5. Hellmich RL, Siegfried BD, Sears MK, Stanley-Horn DE, Daniels MJ, Mattila HR, Lewis LC (2001) Monarch larvae sensitivity to Bacillus thuringiensis-purified proteins and pollen. Proc Natl Acad Sci U S A 98:11925–11930Google Scholar
  6. Johnston CA (2014) Agricultural expansion: land use shell game in the U.S. Northern Plains. Landsc Ecol 29:81–95CrossRefGoogle Scholar
  7. Kingsolver JG, Huey RB (2008) Size, temperature, and fitness: three rules. Evol Ecol Res 10:251–268Google Scholar
  8. Krupke CH, Hunt GJ, Eitzer BD, Andino J, Given K (2012) Multiple routes of pesticide exposure for honey bees living near agricultural fields. PLoS One 7:e29268CrossRefPubMedCentralPubMedGoogle Scholar
  9. Lyons JI, Pierce AA, Barribeau SM, Sternberg ED, Mongue AJ, De Roode JC (2012) Lack of genetic differentiation between monarch butterflies with divergent migration destinations. Mol Ecol 21:3433–3444CrossRefPubMedGoogle Scholar
  10. Nauen R, Ebbinghaus-Kintscher U, Salgado VL, Kaussmann M (2003) Thiamethoxam is a neonicotinoid precursor converted to clothianidin in insects and plants. Pestic Biochem Phys 76:55–69CrossRefGoogle Scholar
  11. Oberhauser KS, Prysby MD, Mattila HR, Stanley-Horn DE, Sears MK, Dively GP, Olson E, Pleasants JM, Lam WF, Hellmich RL (2001) Temporal and spatial overlap between monarch larvae and corn pollen. Proc Natl Acad Sci U S A 98:11913–11918CrossRefPubMedCentralPubMedGoogle Scholar
  12. Pleasants JM, Oberhauser KS (2012) Milkweed loss in agricultural fields because of herbicide use: effect on the monarch butterfly population. Insect Conserv Diver 6:135–144CrossRefGoogle Scholar
  13. Schmuck R, Schoning R, Stork A, Schramel O (2001) Risk posed to honeybees (Apis mellifera L, Hymenoptera) by an imidacloprid seed dressing of sunflowers. Pest Manag Sci 32:1096–1100Google Scholar
  14. Tomizawa M, Casida JE (2005) Neonicotinoid insecticide toxicology: mechanisms of selective action. Annu Rev Pharmacol 45:247–268CrossRefGoogle Scholar
  15. Wright CK, Wimberly MC (2013) Recent land use change in the Western Corn Belt threatens grasslands and wetlands. Proc Natl Acad Sci U S A 110:4134Google Scholar
  16. Zipkin EF, Ries L, Reeves R, Regetz J, Oberhauser KS (2012) Tracking climate impacts on the migratory monarch butterfly. Glo Ch Biol 18:3039–3049CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg (outside the USA) 2015

Authors and Affiliations

  1. 1.Natural Resource ManagementSouth Dakota State UniversityBrookingsUSA
  2. 2.USDA-ARSNorth Central Agricultural Research LaboratoryBrookingsUSA

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