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Naturwissenschaften

, Volume 100, Issue 11, pp 1051–1059 | Cite as

Nest sanitation through defecation: antifungal properties of wood cockroach feces

  • Rebeca B. Rosengaus
  • Kerry Mead
  • William S. Du Comb
  • Ryan W. Benson
  • Veronica G. Godoy
Original Paper

Abstract

The wood cockroach Cryptocercus punctulatus nests as family units inside decayed wood, a substrate known for its high microbial load. We tested the hypothesis that defecation within their nests, a common occurrence in this species, reduces the probability of fungal development. Conidia of the entomopathogenic fungus, Metarhizium anisopliae, were incubated with crushed feces and subsequently plated on potato dextrose agar. Relative to controls, the viability of fungal conidia was significantly reduced following incubation with feces and was negatively correlated with incubation time. Although the cockroach's hindgut contained abundant β-1,3-glucanase activity, its feces had no detectable enzymatic function. Hence, these enzymes are unlikely the source of the fungistasis. Instead, the antifungal compound(s) of the feces involved heat-sensitive factor(s) of potential microbial origin. When feces were boiled or when they were subjected to ultraviolet radiation and subsequently incubated with conidia, viability was “rescued” and germination rates were similar to those of controls. Filtration experiments indicate that the fungistatic activity of feces results from chemical interference. Because Cryptocercidae cockroaches have been considered appropriate models to make inferences about the factors fostering the evolution of termite sociality, we suggest that nesting in microbe-rich environments likely selected for the coupling of intranest defecation and feces fungistasis in the common ancestor of wood cockroaches and termites. This might in turn have served as a preadaptation that prevented mycosis as these phylogenetically related taxa diverged and evolved respectively into subsocial and eusocial organizations.

Keywords

Metarhizium anisopliae Termites Preadaptation Cryptocercus punctulatus β-1,3-Glucanases Sociality 

Notes

Acknowledgments

We thank Drs. John Wenzel, Nan-Yao Su and Thomas Chouvenc for providing Cryptocercus cockroaches. Ashley McGuire helped with the DAPI technique. Dr. Christine Nalepa provided insightful comments during the preparation of this manuscript. We also thank four anonymous reviewers for their helpful comments and suggestions. This work was partially funded by an NSF (DEB 0447316) REU grant to RB Rosengaus and by a Northeastern University TIER1 award to RB Rosengaus, V Godoy, E Cram and M Hincapie. All colonies were contained in a USDA inspected and approved facility. The authors declare that they have no conflict of interest. These experiments comply with the current laws of the United States of America and were conducted under USDA permits.

Author contributions

RBR conceived and designed the experiment, analyzed the data, and wrote the manuscript. KM performed all the germination enumeration and DAPI staining. RBR together with WD, RB, and VG ran the glucanase gels and GDL experiments, while RB was involved in running all filtration experiments.

Supplementary material

114_2013_1110_MOESM1_ESM.docx (181 kb)
ESM 1 (DOCX 180 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Rebeca B. Rosengaus
    • 1
  • Kerry Mead
    • 1
  • William S. Du Comb
    • 2
  • Ryan W. Benson
    • 2
  • Veronica G. Godoy
    • 2
  1. 1.Department of Marine and Environmental SciencesNortheastern UniversityBostonUSA
  2. 2.Department of BiologyNortheastern UniversityBostonUSA

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