Small bite, large impact–saliva and salivary molecules in the medicinal leech, Hirudo medicinalis
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Blood-sucking leeches have been used for medical purposes in humans for hundreds of years. Accordingly, one of the most prominent species has been named Hirudo medicinalis by Carl Linne in 1758. Feeding on vertebrate blood poses some serious problems to blood-sucking ectoparasites, as they have to penetrate the body surface of the host and to suppress the normal reactions of the host to such injuries (swelling, pain, inflammation) to remain undetected during the feeding period. Furthermore, the parasites have to take measures to inhibit the normal reactions in host tissues to blood vessel damage, namely hemostasis and blood coagulation (platelet aggregation and activation, activation of thrombin and formation of fibrin clots). During evolution, leeches have acquired the ability to control these processes in their hosts by transferring various bioactive substances to the host. These substances are supposedly produced in unicellular salivary gland cells and injected into the wound at the feeding site through tiny salivary ductule openings in the jaws that the leech uses to slice open the host body surface and to cut blood vessels in the depth of the wound. This review summarizes current knowledge about the salivary gland cells and the biological effects of individual saliva components as well as hints to the potential usefulness of some of these compounds for medical purposes.
KeywordsHirudo spp. Salivary gland cells Saliva Blood feeding Salivary proteins Medical applications
Due to limitations in space, the authors were not able to cite every single piece of work of other researchers in the field and have focussed on the most influential publications. We apologize for every important contribution that we may have overlooked. The authors would like to thank Gabriele Uhl and Christine Putzar for their cooperation in the histological project and Steffen Harzsch and Verena Rieger for their help with the histamine immune fluorescence. Thanks to Detlef Menzel (BioRepro GmbH, Potsdam) for providing the animals. We are thankful to the four reviewers for their constructive and helpful comments. Sarah Lemke is the recipient of a doctoral stipend from the Konrad Adenauer-Stiftung, Germany.
We declare that the experiments described in this paper comply with the current laws in Germany.
Conflicts of interest
The authors declare that they have no conflict of interest.
- Damas D (1972) Durcissement de la cuticule des machoires chez Hirudo medicinalis (Annelide, Hirudinee). Aboutissant aux structures dentaires etude histochemique et ultrastructurale. Arch Zool Exp Gen 113:401–421Google Scholar
- Damas D (1974) Etude histologique et histochemique des glandes salivaires de la sangsue medicinale, Hirudo medicinalis (Hirudinee, Gnathobdelle). Arch Zool Exp Gen 115:279–292Google Scholar
- Domogalla B (2005) NMR-Lösungsstruktur des Proteins Saratin, strukturelle Charakterisierung der Saratin-Kollagen-Interaktion und des Carausius morosus-hyperthrehalosämischen Hormons (Cam-HrTH-I). Dissertation, University of RegensburgGoogle Scholar
- Fink E, Rehm H, Gippner C, Bode W, Eulitz M, Machleidt W, Fritz H (1986) The primary structure of bdellin B-3 from the leech Hirudo medicinalis. Bdellin B-3 is a compact proteinase inhibitor of a “non-classical” Kazal type. It is present in the leech in a high molecular mass form. Biol Chem Hoppe Seyler 367:1235–1242PubMedCrossRefGoogle Scholar
- Fradkov A, Berezhnoy S, Barsova E, Zavalova L, Lukyanov S, Baskova I, Sverdlov ED (1996) Enzyme from the medicinal leech (Hirudo medicinalis) that specifically splits endo-[epsilon](-[gamma]-Glu)-Lys isopeptide bonds: cDNA cloning and protein primary structure. FEBS Lett 390:145–148PubMedCrossRefGoogle Scholar
- Haycraft JB (1884) On the action of a secretion obtained from the medicinal leech on the coagulation of the blood. Proc R Soc Lond B 36:478–487Google Scholar
- Major RH (1954) A history of medicine. Charles Thomas, Springfield, IllinoisGoogle Scholar
- Mann KH (1962) Leeches (Hirudinea). Pergamon, OxfordGoogle Scholar
- Moser M, Auerswald E, Mentele R, Eckerskorn C, Fritz H, Fink E (1998) Bdellastasin, a serine protease inhibitor of the antistasin family from the medical leech (Hirudo medicinalis)—primary structure, expression in yeast, and characterisation of native and recombinant inhibitor. Eur J Biochem 253:212–220PubMedCrossRefGoogle Scholar
- Oppler P (1904) Feinere Anatomie der im Kopf- und Halsteil von Hirudo vorkommenden Drüsen. Dissertation, University of BernGoogle Scholar
- Reverter D, Vendrell J, Canals F, Horstmann J, Aviles FX, Fritz H, Sommerhoff CP (1998) A carboxypeptidase inhibitor from the medical leech Hirudo medicinalis. Isolation, sequence analysis, cDNA cloning, recombinant expression, and characterization. J Biol Chem 273:32927–32933PubMedCrossRefGoogle Scholar
- Rigbi M, Levy H, Eldor A, Iraqi F, Teitelbaum M, Orevi M, Horovitz A, Galun R (1987a) The saliva of the medicinal leech Hirudo medicinalis—II. Inhibition of platelet aggregation and of leukocyte activity and examination of reputed anaesthetic effects. Comp Biochem Physiol C 88:95–98CrossRefGoogle Scholar
- Sawyer RT (1986a) Leech biology and behaviour 1: anatomy, physiology, and behaviour. Oxford University Press, OxfordGoogle Scholar
- Sawyer RT (1986b) Leech biology and behaviour 2: feeding biology, ecology and systematics. Oxford University Press, OxfordGoogle Scholar
- Snider GL, Stone PJ, Lucey EC, Breuer R, Calore JD, Seshadri T, Catanese A, Maschler R, Schnebli HP (1985) Eglin-c, a polypeptide derived from the medicinal leech, prevents human neutrophil elastase-induced emphysema and bronchial secretory cell metaplasia in the hamster. Am Rev Respir Dis 132:1155–1161PubMedGoogle Scholar
- Stubbs MT, Morenweiser R, Stürzebecher J, Bauer M, Bode W, Huber R, Piechottka GP, Matschiner G, Sommerhoff CP, Fritz H, Auerswald EA (1997) The three-dimensional structure of recombinant leech-derived tryptase inhibitor in complex with trypsin. Implications for the structure of human mast cell tryptase and its inhibition. J Biol Chem 272:19931–19937PubMedCrossRefGoogle Scholar
- Wenning A (1996) Managing high salt loads: from neuron to urine in the leech. Physiol Zool 69:719–745Google Scholar
- Yip J, Shen Y, Andrews RK (2004) Primary platelet adhesion receptors. Australian Biochemist 35:4–8Google Scholar
- Zavalova LL, Baskova IP, Lukyanov SA, Sass AV, Snezhkov EV, Akopov SB, Artamonova II, Archipova VS, Nesmeyanov VA, Kozlov DG, Benevolensky SV, Kiseleva VI, Poverenny AM, Sverdlov ED (2000) Destabilase from the medicinal leech is a representative of a novel family of lysozymes. Biochim Biophys Acta–Prot Struct Mol Enzymol 1478:69–77CrossRefGoogle Scholar
- Zerbst-Boroffka I, Wenning A (1986) Mechanisms of regulatory salt and water excretion in the leech, Hirudo medicinalis L. Zool Beitr NF 30:359–377Google Scholar