Naturwissenschaften

, Volume 98, Issue 6, pp 473–492 | Cite as

Role of RNA interference in plant improvement

  • Umesh Balkrishna Jagtap
  • Ranjit Gajanan Gurav
  • Vishwas Anant Bapat
Review

Abstract

Research to alter crops for their better performance involving modern technology is underway in numerous plants, and achievements in transgenic plants are impacting crop improvements in unparalleled ways. Striking progress has been made using genetic engineering technology over the past two decades in manipulating genes from diverse and exotic sources, and inserting them into crop plants for inducing desirable characteristics. RNA interference (RNAi) has recently been identified as a natural mechanism for regulation of gene expression in all higher organisms from plants to humans and promises greater accuracy and precision to plant improvement. The expression of any gene can be down-regulated in a highly explicit manner exclusive of affecting the expression of any other gene by using RNAi technologies. Additional research in this field has been focused on a number of other areas including microRNAs, hairpin RNA, and promoter methylation. Manipulating new RNAi pathways, which generate small RNA molecules to amend gene expression in crops, can produce new quality traits and having better potentiality of protection against abiotic and biotic stresses. Nutritional improvement, change in morphology, or enhanced secondary metabolite synthesis are some of the other advantages of RNAi technology. In addition to its roles in regulating gene expression, RNAi is also used as a natural defense mechanism against molecular parasites such as jumping genes and viral genetic elements that affect genome stability. Even though much advancement has been made on the field of RNAi over the preceding few years, the full prospective of RNAi for crop improvement remains to be fully realized. The intricacy of RNAi pathway, the molecular machineries, and how it relates to plant development are still to be explained.

Keywords

Co-suppression Crop improvement Gene silencing RNA interference Stress Transgene 

References

  1. Achard P, Herr A, Baulcombe DC, Harberd NP (2004) Modulation of floral development by gibberellin-regulated microRNA. Development 131:3357–3365PubMedCrossRefGoogle Scholar
  2. Allen RS, Millgate AG, Chitty JA, Thisleton J, Miller JAC, Fist AG, Gerlach WA, Larkin PJ (2004) RNAi-mediated replacement of morphine with the non narcotic alkaloid reticuline in Opium poppy. Nat Biotechnol 22:1559–1566PubMedCrossRefGoogle Scholar
  3. Allen RS, James MAC, Julie CA, Anthony FJ, Wayne GL, Philip LJ (2008) Metabolic engineering of morphinan alkaloids by overexpression and RNAi suppression of salutaridinol 7-O-acetyltransferase in Opium poppy. Plant Biotech J 6:22–30Google Scholar
  4. Andika IB, Kondo H, Tamada T (2005) Evidence that RNA silencing-mediated resistance to beet necrotic yellow vein virus is less effective in roots than in leaves. Mol Plant Microbe Interact 18:194–204PubMedCrossRefGoogle Scholar
  5. Auer C, Frederick R (2009) Crop improvement using small RNAs: applications and predictive ecological risk assessments. Trends Biotechnol 27:644–651PubMedCrossRefGoogle Scholar
  6. Bapat VA, Trivedi PK, Ghosh A, Sane VA, Ganapathi TR, Nath P (2010) Ripening of fleshy fruit: molecular insight and the role of ethylene. Biotech Adv 28:94–107CrossRefGoogle Scholar
  7. Bartel DP (2004) MicroRNAs: genomics, biogenesis, mechanism and function. Cell 116:281–297PubMedCrossRefGoogle Scholar
  8. Baum JA, Bogaert T, Clinton W, Heck GR, Feldmann P, Ilagan O, Johnson S, Plaetinck G, Munyikwa T, Pleau M (2007) Control of coleopteran insect pests through RNA interference. Nat Biotech 25(11):1322–1326CrossRefGoogle Scholar
  9. Baumberger N, Baulcombe DC (2005) Arabidopsis ARGONAUTE1 is an RNA slicer that selectively recruits microRNAs and short interfering RNAs. Proc Natl Acad Sci USA 102:11928–11933PubMedCrossRefGoogle Scholar
  10. Belostotsky DA, Sieburth LE (2009) Kill the messenger: mRNA decay and plant development. Curr Opin Plant Biol 12:96–102PubMedCrossRefGoogle Scholar
  11. Boisson-Dernier A, Chabaud M, Garcia F, Becard G, Rosenberg C, Barker DG (2001) Agrobacterium rhizogenes-transformed roots of Medicago truncatula for the study of nitrogen-fixing andendomycorrhizal symbiotic associations. Mol Plant Microbe Interact 14:695–700PubMedCrossRefGoogle Scholar
  12. Borgio JF (2009) RNA interference (RNAi) technology: a promising tool for medicinal plant research. J Medicinal Plant Res 3:1176–1183Google Scholar
  13. Bravo A, Soberôn M (2008) How to cope with insect resistance to Bt-toxins.Trends Biotechnol 26:573–579Google Scholar
  14. Brown ME, Funk CC (2008) Climate: food security under climate change. Science 319:580–581PubMedCrossRefGoogle Scholar
  15. Camp WV (2005) Yield enhancement genes: seeds for growth. Curr Opin Biotechnol 16:147–153PubMedCrossRefGoogle Scholar
  16. Canter PH, Thomas H, Ernst E (2005) Bringing medicinal plants into cultivation: opportunities and challenges for biotechnology. Trends Biotechnol 23:180–185PubMedCrossRefGoogle Scholar
  17. Chiou TJ, Aung K, Lin S, Wu CC, Chiang SF, Su CL (2006) Regulation of phosphate homeostasis by microRNA in Arabidopsis. Plant Cell 18:412–421PubMedCrossRefGoogle Scholar
  18. Coles JP, Phillips AL, Croker SJ, Garcia-Lepe R, Lewis MJ, Hedden P (1999) Modification of gibberellin production and plant development in Arabidopsis by sense and antisense expression of gibberellin 20-oxidase genes. Plant J 17:547–556PubMedCrossRefGoogle Scholar
  19. Cushman JC, Bohnert HJ (2000) Genomic approaches to plant stress tolerance. Curr Opin Plant Biol 3:117–124PubMedCrossRefGoogle Scholar
  20. Dafny-Yelin M, Chung SM, Frankman EL, Tzfira T (2007) pSAT RNA interference vectors: a modular series for multiple gene down regulation in plants. Plant Physiol 145:1272–1281PubMedCrossRefGoogle Scholar
  21. Da-Hong L, Hui L, Yan-li Y, Ping-ping Z, Jian-sheng L (2009) Down-regulated expression of RACK1 gene by RNA interference enhances drought tolerance in rice. Rice Sci 16:14–20CrossRefGoogle Scholar
  22. de Dorlodot S, Forster B, Pages L, Price A, Tuberosa R, Draye X (2007) Root system architecture: opportunities and constraints for genetic improvement of crops. Trends Plant Sci 12:474–481PubMedCrossRefGoogle Scholar
  23. de Framond A, Rich PJ, McMillan J, Ejeta G (2007) Effects on Striga parasitism of transgenic maize armed with RNAi constructs targeting essential S. asiatica genes. In: Ejeta G, Gressel J (eds) Integrating new technologies for Striga control: towards ending the witch-hunt. World Scientific, Singapore, pp 185–196CrossRefGoogle Scholar
  24. De Jong M, Wolters-Arts M, Feron R, Mariani C, Vriezen WH (2009) The Solanum lycopersicum auxin response factor 7 (SlARF7) regulates auxin signaling during tomato fruit set and development. Plant J 5:160–170CrossRefGoogle Scholar
  25. Dexter R, Qualley A, Kish CM (2007) Characterization of a petunia acetyltransferase involved in the biosynthesis of the floral volatile isoeugenol. Plant J 49:265–275PubMedCrossRefGoogle Scholar
  26. Dodo HW, Konan KN, Chen FC, Egnin M, Viquez OM (2008) Alleviating peanut allergy using genetic engineering: the silencing of the immunodominant allergen Ara h 2 leads to its significant reduction and a decrease in peanut allergenicity. Plant Biotech J 6:135–145CrossRefGoogle Scholar
  27. Dunoyer P, Himber C, Voinnet O (2006) Induction, suppression and requirement of RNA silencing pathways in virulent Agrobacterium tumefaciens infections. Nat Genet 38(2):258–263PubMedCrossRefGoogle Scholar
  28. Eck JV, Conlin B, Garvin DF, Mason H, Navarre DA, Brown CR (2007) Enhanced beta-carotene content in potato via RNAi silencing of the beta-carotene hydroxylase gene. Am J Potato Res 84:l331–l1342CrossRefGoogle Scholar
  29. Fairbairn DJ, Cavallaro AS, Bernard M, Mahalinga-Iyer J, Graham MW, Botella JR (2007) Host-delivered RNAi: an effective strategy to silence genes in plant parasitic nematodes. Planta 226:1525–1533PubMedCrossRefGoogle Scholar
  30. Fire A, Xu S, Montgomery MK, Kostas SA, Driver SE, Mello CC (1998) Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391:806–811PubMedCrossRefGoogle Scholar
  31. Fuentes A, Ramos PL, Fiallo E, Callard D, Sanchez Y, Peral R, Rodriguez R, Pujol M (2006) Intron-hairpin RNA derived from replication associated protein C1 gene confers immunity to tomato yellow leaf curl virus infection in transgenic tomato plants. Transgen Res 15:291–304CrossRefGoogle Scholar
  32. Fujii S, Toriyama K (2008) DCW11, down-regulated gene 11 in CW-type cytoplasmic male sterile rice, encoding mitochondrial protein phosphatase 2C is related to CMS. Plant Cell Physiol 49:633–640PubMedCrossRefGoogle Scholar
  33. Fujii H, Chiou TJ, Lin SI, Aung K, Zhu JK (2005) A miRNA involved in phosphate starvation response in Arabidopsis. Curr Biol 15:2038–2043PubMedCrossRefGoogle Scholar
  34. Fujii N, Inui T, Iwasa K, Morishige T, Sato F (2007a) Knockdown of berberine bridge enzyme by RNAi accumulates (S)-reticuline and activates a silent pathway in cultured California poppy cells. Transgenic Res 16:363–375PubMedCrossRefGoogle Scholar
  35. Fujii S, Komatsu S, Toriyama K (2007b) Retrograde regulation of nuclear gene expression in CW–CMS of rice. Plant Mol Biol 63:405–417PubMedCrossRefGoogle Scholar
  36. Fukusaki E, Kawasaki K, Kajiyama S, An CI, Suzuki K, Tanaka Y, Kobayashi A (2004) Flower color modulations of Torenia hybrida by downregulation of chalcone synthase genes with RNA interference. J Biotech 111:229–240CrossRefGoogle Scholar
  37. Gil-Humanes J, Pisto’n F, Hernando A, Alvarez JB, Shewry PR, Barro F (2008) Silencing of g-gliadins by RNA interference (RNAi) in bread wheat. J Cereal Sci 48:565–568CrossRefGoogle Scholar
  38. Gilissen LJ, Bolhaar ST, Matos CI, Rouwendal GJ, Boone MJ, Krens FA, Zuidmeer L, Van Leeuwen A, Akkerdaas J, Hoffmann-Sommergruber K, Knulst AC, Bosch D, Van de Weg WE, Van Ree R (2005) Silencing the major apple allergen Mal d 1 by using the RNA interference approach. J Allergy Clin Immunol 115:364–369PubMedCrossRefGoogle Scholar
  39. Godfray HCJ, Beddington JR, Crute IR, Haddad L, Lawrence D, Muir JZ, Pretty J, Robinson S, Thomas SM, Toulmin C (2010) Food security: the challenge of feeding 9 billion people. Science 327:812–818PubMedCrossRefGoogle Scholar
  40. Gomez-Galera S, Pelacho AM, Gene A, Capell T, Christou P (2007) The genetic manipulation of medicinal and aromatic plants. Plant Cell Rep 26:1689–1715PubMedCrossRefGoogle Scholar
  41. Griesser M, Hoffmann T, Bellido ML, Rosati C, Fink B, Kurtze R, Aharoni A, Munoz-Blanco J, Schwab W (2008) Redirection of flavonoid biosynthesis through the down-regulation of an anthocyanidin glucosyltransferase in ripening strawberry fruit. Plant Physiol 146:1528–1539PubMedCrossRefGoogle Scholar
  42. Gronquist M, Bezzerides A, Attygalle A, Meinwald J, Eisner M, Eisner T (2001) Attractive and defensive functions of the ultraviolet pigments of a flower (Hypericum calycinum). Proc Natl Acad Sci USA 98:13745–13750PubMedCrossRefGoogle Scholar
  43. Hamilton AJ, Baulcombe DC (1999) A species of small antisense RNA in posttranscriptional gene silencing in plants. Science 286:950–952PubMedCrossRefGoogle Scholar
  44. Han JY, Kwon YS, Yang DC, Jung YR, Choi YE (2006) Expression and RNA interference-induced silencing of the dammarenediol synthase gene in Panax ginseng. Plant Cell Physiol 47:1653–1662PubMedCrossRefGoogle Scholar
  45. Hedden P, Phillips AL (2000) Manipulation of hormone biosynthetic genes in transgenic plants. Curr Opin Biotechnol 11:130–137PubMedCrossRefGoogle Scholar
  46. Herdt RW (2006) Biotechnology in agriculture. Ann Rev Environ Resour 31:265–295CrossRefGoogle Scholar
  47. Herman EM, Helm RM, Jung R, Kinney AJ (2003) Genetic modification removes an immunodominant allergen from soybean. Plant Physiol 132:36–43PubMedCrossRefGoogle Scholar
  48. Hernández I, Chacón O, Rodriguez R, Portieles R, Pujol YLM, Borrás-Hidalgo O (2009) Black shank resistant tobacco by silencing of glutathione S-transferase. Biochem Biophys Res Commun 387:300–304PubMedCrossRefGoogle Scholar
  49. Hily JM, Ravelonandro M, Damsteegt V, Basset C, Petri C, Liu Z, Scorza R (2007) Plum pox virus coat protein gene intron-hairpin-RNA (ihpR NA) constructs provide resistance to plum pox virus in Nicotiana bethamiana and Prunus domestica. J Am Soc Horti Sci 132:850–858Google Scholar
  50. Hirschi K (2008) Nutritional improvements in plants: time to bite on biofortified foods. Trends Plant Sci 13:459–463PubMedCrossRefGoogle Scholar
  51. Hu Y, Qin F, Huang L, Sun Q, Li C, Zhao Y, Zhou D (2009) Rice histone deacetylase genes display specific expression patterns and developmental functions. Biochem Biophys Res Commun 388:266–271PubMedCrossRefGoogle Scholar
  52. Huang G, Allen R, Davis EL, Baum TJ, Hussey RS (2006) Engineering broad root-knot resistance in transgenic plants by RNAi silencing of a conserved and essential root-knot nematode parasitism gene. Proc Natl Acad Sci USA 103:14302–14306PubMedCrossRefGoogle Scholar
  53. Hüsken A, Baumert A, Milkowski C, Becker HC, Strack D, Möllers C (2005) Resveratrol glucoside (Piceid) synthesis in seeds of transgenic oilseed rape (Brassica napus L.). Theor Appl Genet 111:1553–1562PubMedCrossRefGoogle Scholar
  54. Jian X, Zhang L, Li G, Zhang L, Wang X, Cao X, Fang X, Zha FC (2010) Identification of novel stress-regulated microRNAs from Oryza sativa L. Genomics 95:47–50PubMedCrossRefGoogle Scholar
  55. Johansson SG, Bieber T, Dahl R, Friedmann PS, Lanier BQ, Lockey RF, Motala C, Ortega Martell JA, Platts-Mills TA, Ring J, Thien F, Van Cauwenberge P, Williams HC (2004) Revised nomenclature for allergy for global use: report of the nomenclature review committee of the world allergy organization. J Allergy Clin Immunol 113:832–836PubMedCrossRefGoogle Scholar
  56. Jones-Rhoades MW, Bartel DP (2004) Computational identification of plant micro-RNAs and their targets, including a stress-induced miRNA. Mol Cell 14:787–799PubMedCrossRefGoogle Scholar
  57. Jørgensen K, Bak S, Busk PK, Sørensen C, Olsen CE, Puonti-Kaerlas J, Moller BL (2005) Cassava plants with a depleted cyanogenic glucoside content in leaves and tubers. Plant Physiol 139:363–374PubMedCrossRefGoogle Scholar
  58. Kamachi S, Mochizuki A, Nishiguchi M, Tabei Y (2007) Transgenic Nicotiana benthamiana plants resistant to cucumber green mottle mosaic virus based on RNA silencing. Plant Cell Rep 26:1283–1288PubMedCrossRefGoogle Scholar
  59. Kaminaga Y, Schnepp J, Peel G, Kish CM, Ben-Nissan G, Weis D, Orlova I, Lavie O, Rhode D, Wood K, Porterfield DM, Cooper AJL, Schloss JV, Pichersky E, Vainstein A, Dudareva N (2006) Phenylacetaldehyde synthase from Petunia hybrida is a biofunctional enzyme that catalyzes the efficient coupling of phenylalanine decarboxylation to phenylalanine oxidation. J Biol Chem 281:23357–23366PubMedCrossRefGoogle Scholar
  60. Kempe K, Higashi Y, Frick S, Sabarna K, Kutchan TM (2009) RNAi suppression of the morphine biosynthetic gene salAT and evidence of association of pathway enzymes. Phytochemistry 70:579–589PubMedCrossRefGoogle Scholar
  61. Kertbundit S, Pongtanom N, Ruanjan P, Chantasingh D, Tanwanchai A, Panyim S (2007) Resistance of transgenic papaya plants to papaya ringspot virus. Biologia Plantarum 51:333–339CrossRefGoogle Scholar
  62. Khush GS (1999) Green revolution: preparing for the 21st century. Genome 42:646–655PubMedCrossRefGoogle Scholar
  63. Kim YS, Lee YH, Kim HS, Kim MS, Hahn KW, Ko JH, Joung H, Jeon JH (2008) Development of patatin knockdown potato tubers using RNA interference (RNAi) technology, for the production of human-therapeutic glycoproteins. BMC Biotechnol 8:36PubMedCrossRefGoogle Scholar
  64. Koeduka T, Fridman E, Gang DR, Vassa DG, Jackson BL, Kish CM, Orlova I, Spassova SM, Lewis NG, Noel JP, Baiga TJ, Dudareva N, Pichersky E (2006) Eugenol and isoeugenol, characteristic aromatic constituents of spices, are biosynthesized via reduction of a coniferyl alcohol ester. Proc Natl Acad Sci USA 103:10128–10133PubMedCrossRefGoogle Scholar
  65. Koseki M, Goto K, Masuta C, Kanazawa A (2005) The star-type colour pattern in Petunia hybrida ‘red star’ flowers is induced by sequence-specific degradation of chalcone synthase RNA. Plant Cell Physiol 46:1879–1883PubMedCrossRefGoogle Scholar
  66. Krubphachaya P, Juříček M, Kertbundit S (2007) Induction of RNA-mediated resistance to papaya ring spot virus type W. J Biochem Mol Biol 40:401–411CrossRefGoogle Scholar
  67. Kusaba M (2004) RNA interference in crop plants. Curr Opin Biotechnol 15:139–143Google Scholar
  68. Kusaba M, Miyahara K, Iida S, Fukuoka H, Takano T, Sassa H, Nishimura M, Nishio T (2003) Low glutelin content 1: a dominant mutation that suppresses the glutelin multigene family via RNA silencing in rice. Plant Cell 15:1455–1467PubMedCrossRefGoogle Scholar
  69. Le LQ, Lorenz Y, Scheurer S, Fotisch K, Enrique E, Bartra J, Biemelt S, Vieths S, Sonnewald U (2006a) Design of tomato fruits with reduced allergenicity by dsRNAi-mediated inhibition of ns-LTP (Lyc e 3) expression. Plant Biotech J 4:231–242CrossRefGoogle Scholar
  70. Le LQ, Mahler V, Lorenz Y, Scheurer S, Biemelt S, Vieths S, Sonnewald U (2006b) Reduced allergenicity of tomato fruits harvested from Lyc e 1-silenced transgenic tomato plants. J Allergy Clin Immunol 118:1176–1183PubMedCrossRefGoogle Scholar
  71. Lewis RS, Jack AM, Morris JW, Robert VJM, Gavilano LB, Siminszky B, Bush LP, Hayes AJ, Dewey RE (2008) RNA interference (RNAi)-induced suppression of nicotine demethylase activity reduces levels of a key carcinogen in cured tobacco leaves. Plant Biotech J 6:346–354CrossRefGoogle Scholar
  72. Li JC, Guo JB, Xu WZ, Ma M (2007) RNA interference-mediated silencing of phytochelatin synthase gene reduces cadmium accumulation in rice seeds. J Integrative Plant Biol 49:1032–1037CrossRefGoogle Scholar
  73. Liu Q, Singh SP, Green AG (2002) High-stearic and high-oleic cottonseed oils produced by hairpin RNA-mediated post-transcriptional gene silencing. Plant Physiol 129:1732–1743PubMedCrossRefGoogle Scholar
  74. Lobell DB, Burke MB, Tebaldi C, Mastrandrea MD, Falcon WP, Naylor RL (2008) Prioritizing climate change adaptation needs for food security in 2030. Science 319:607–610PubMedCrossRefGoogle Scholar
  75. López C, Cervera M, Fagoaga C, Moreno P, Navarro L, Flores R, Peña L (2010) Accumulation of transgene-derived siRNAs is not sufficient for RNAi-mediated protection against Citrus tristeza virus in transgenic Mexican lime. Mol Plant Pathol 11:33–41PubMedCrossRefGoogle Scholar
  76. Mansoor S, Amin I, Hussain M, Zafar Y, Briddon RW (2006) Engineering novel traits in plants through RNA interference. Trends Plant Sci 11:559–565PubMedCrossRefGoogle Scholar
  77. Mao YB, Cai WJ, Wang JW, Hong GJ, Tao XY, Wang LJ, Huang YP, Chen XY (2007) Silencing a cotton bollworm P450 monooxygenase gene by plant-mediated RNAi impairs larval tolerance of gossypol. Nat Biotech 25(11):1307–1313CrossRefGoogle Scholar
  78. Missiou A, Kalantidis K, Boutla A, Tzortzakaki S, Tabler M, Tsagris M (2004) Generation of transgenic potato plants highly resistant to potato virus Y (PVY) through RNA silencing. Mol Breeding 14:185–197CrossRefGoogle Scholar
  79. Mittler R, Blumwald E (2010) Genetic engineering for modern agriculture: challenges and perspectives. Ann Rev Plant Biol 61:443–462CrossRefGoogle Scholar
  80. Molesini B, Rotino GL, Spena A, Pandolfini T (2009) Expression profile analysis of early fruit development in iaaM-parthenocarpic tomato plants. BMC Res Notes 2:1–7CrossRefGoogle Scholar
  81. Moritoh S, Miki D, Akiyama M, Kawahara M, Izawa T, Maki H, Shimamoto K (2005) RNAi-mediated silencing of OsGEN-L (OsGEN-like), a new member of the RAD2/XPG nuclease family, causes male sterility by defect of microspore development in rice. Plant Cell Physiol 46:699–715PubMedCrossRefGoogle Scholar
  82. Nakatsuka T, Mishiba KI, Kubota A, Abe Y, Yamamura S, Nakamura N, Tanaka Y, Nishihara M (2010) Genetic engineering of novel flower colour by suppression of anthocyanin modification genes in gentian. J Plant Physiol 167:231–237PubMedCrossRefGoogle Scholar
  83. Napoli C, Lemieux C, Jorgensen R (1990) Introduction of a chimeric chalcone synthase gene into Petunia results in reversible co-suppression of homologous genes in trans. Plant Cell 2:279–289PubMedCrossRefGoogle Scholar
  84. Navarro L, Dunoyer P, Jay F, Arnold B, Dharmasiri N, Estelle M, Voinnet O, Jones JD (2006) A plant miRNA contributes to antibacterial resistance by repressing auxin signalling. Science 312:436–439PubMedCrossRefGoogle Scholar
  85. Nawaz-ul-Rehman MS, Mansoor S, Khan AA, Zafar Y, Briddon RW (2007) RNAi-mediated male sterility of tobacco by silencing TA29. Mol Biotechnol 36:159–165PubMedCrossRefGoogle Scholar
  86. Nazmul-Haque AKM, Tanaka Y, Sonoda S, Nishiguchi M (2007) Analysis of transitive RNA silencing after grafting in transgenic plants with the coat protein gene of sweet potato feathery mottle virus. Plant Mol Biol 63:35–47CrossRefGoogle Scholar
  87. Nishihara M, Nakatsuka T, Yamamura S (2005) Flavonoid components and flower color change in transgenic tobacco plants by suppression of chalcone isomerase gene. FEBS Lett 579:6074–6078PubMedCrossRefGoogle Scholar
  88. Noris E, Lucioli A, Tavazza R, Caciagli P, Accotto GP, Tavazza M (2004) Tomato yellow leaf curl Sardinia virus can overcome transgene-mediated RNA silencing of two essential viral genes. J Gen Virol 85:1745–1749PubMedCrossRefGoogle Scholar
  89. Nunes ACS, Giovanni R, Cuneo VF, Amaya-Farfan J, de Capdeville G, Rech EL, Aragao FJL (2006) RNAi-mediated silencing of the myo-inositol-1-phosphate synthase gene (GmMIPS1) in transgenic soybean inhibited seed development and reduced phytate content. Planta 224:125–132PubMedCrossRefGoogle Scholar
  90. Ogita S, Uefuji H, Yamaguchi Y, Koizumi N, Sano H (2003) RNA interference: producing decaffeinated coffee plants. Nature 423:823PubMedCrossRefGoogle Scholar
  91. Ogita S, Uefuji H, Morimoto M, Sano H (2004) Application of RNAi to confirm theobromine as the major intermediate for caffeine biosynthesis in coffee plants with potential for construction of decaffeinated varieties. Plant Mol Biol 54:931–941PubMedCrossRefGoogle Scholar
  92. Orlova I, Marshall-Colón A, Schnepp J, Wood B, Varbanova M, Fridman E, Blakeslee JJ, Peer WA, Murphy AS, Rhodes D, Pichersky E, Dudareva N (2006) Reduction of benzenoid synthesis in petunia flowers reveals multiple pathways to benzoic acid and enhancement in auxin transport. Plant Cell 18:3458–3475PubMedCrossRefGoogle Scholar
  93. Pardo JM (2010) Biotechnology of water and salinity stress tolerance. Curr Opin Biotechnol 21:1–12CrossRefGoogle Scholar
  94. Pichersky E, Dudareva N (2007) Scent engineering: toward the goal of controlling how flowers smell. Trends Biotechnol 25:105–110PubMedCrossRefGoogle Scholar
  95. Pooggin M, Shivaprasad PV, Veluthambi K, Hohn T (2003) RNAi targeting of DNA virus in plants. Nature Biotechnol 21:131–132CrossRefGoogle Scholar
  96. Qiao F, Yang Q, Wang C, Fan Y, Wu X, Zhao K (2007) Modification of plant height via RNAi suppression of OsGA20ox2 gene in rice. Euphytica 158:35–45CrossRefGoogle Scholar
  97. Rahman M, Ali I, Husnain T, Riazuddin S (2008) RNA interference: the story of gene silencing in plants and humans. Biotech Adv 26:202–209CrossRefGoogle Scholar
  98. Regina A, Bird A, Topping D, Bowden S, Freeman J, Barsby T, Kosar-Hashemi B, Li Z, Rahman S, Morell M (2006) High-amylose wheat generated by RNA interference improves indices of large-bowel health in rats. Proc Natl Acad Sci USA 103:3546–3551PubMedCrossRefGoogle Scholar
  99. Regina A, Kosar-Hashemi B, Ling S, Li Z, Rahman S, Morell M (2010) Control of starch branching in barley defined through differential RNAi suppression of starch branching enzyme IIa and IIb. J Exp Bot 61:1469–1482PubMedCrossRefGoogle Scholar
  100. Reyes JL, Chua NH (2007) ABA induction of miR159 controls transcript levels of two MYB factors during Arabidopsis seed germination. Plant J 49:592–606PubMedCrossRefGoogle Scholar
  101. Riechen J (2007) Establishment of broad-spectrum resistance against Blumeria graminis f. sp. tritici in Triticum aestivum by RNAi-mediated knock-down of MLO. J Verbraucherschutz und Lebensmittelsicherheit 2:120CrossRefGoogle Scholar
  102. Romano N, Macino G (1992) Quelling: transient inactivation of gene expression in Neurospora crassa by transformation with homologous sequences. Mol Microbiol 22:3343–3353CrossRefGoogle Scholar
  103. Sano T, Matsuura Y (2004) Accumulation of short interfering RNAs characteristic of RNA silencing precedes recovery of tomato plants from severe symptoms of potato spindle tuber viroid infection. J Gen Plant Pathol 70:50–53CrossRefGoogle Scholar
  104. Schijlen EGWM, de Vos RCH, Martens S, Jonker HH, Rosin FM, Molthoff JW, Tikunov YM, Angenent GC, van Tunen AJ, Bovy AG (2007) RNA interference silencing of chalcone synthase, the first step in the flavonoid biosynthesis pathway, leads to parthenocarpic tomato fruits. Plant Physiol 144:1520–1530PubMedCrossRefGoogle Scholar
  105. Schwind N, Zwiebel M, Itaya A, Ding B, Wang M, Krczal G, Wassenegger M (2009) RNAi-mediated resistance to potato spindle tuber viroid in transgenic tomato expressing a viroid hairpin RNA construct. Mol Plant Pathol 10:459–469PubMedCrossRefGoogle Scholar
  106. Segal G, Song R, Messing J (2003) A new opaque variant of maize by a single dominant RNA-interference inducing transgene. Genetics 165:387–397PubMedGoogle Scholar
  107. Seitz C, Vitten M, Steinbach P, Hartl S, Hirsche J, Rathje W, Treutter D, Forkmann G (2007) Redirection of anthocyanin synthesis in Osteospermum hybrida by a two-enzyme manipulation strategy. Phytochem 68:824–833CrossRefGoogle Scholar
  108. Shao Y, Zhu HL, Tian HQ, Wang XG, Lin XJ, Zhu BJ, Xie YH, Luo YB (2008) Virus induced gene silencing in plant species. Russian J Plant Physiol 55(2):168–174CrossRefGoogle Scholar
  109. Sharma HC, Crouch JH, Sharma KK, Seetharama N, Hash CT (2002) Applications of biotechnology for crop improvement: prospects and constraints. Plant Sci 163:381–395CrossRefGoogle Scholar
  110. Shepherd DN, Martin DP, Thomson JA (2009) Transgenic strategies for developing crops resistant to geminivirus. Plant Sci 176:1–11Google Scholar
  111. Shukla LI, Chinnusamy V, Sunkar R (2008) The role of microRNAs and other endogenous small RNAs in plant stress responses. Biochim Biophys Acta 1779:743–748PubMedGoogle Scholar
  112. Sicherer SH, Sampson HA (2010) Food allergy. J Allergy Clin Immunol 125:116–125CrossRefGoogle Scholar
  113. Sindhu AS, Maier TR, Mitchum MG, Hussey RS, Davis EL, Baum TJ (2009) Effective and specific in planta RNAi in cyst nematodes: expression interference of four parasitism genes reduces parasitic success. J Exp Bot 60:315–324PubMedCrossRefGoogle Scholar
  114. Sunilkumar G, Campbell LM, Puckhaber L, Stipanovic RD, Rathore KS (2006) Engineering cottonseed for use in human nutrition by tissue-specific reduction of toxic gossypol. Proc Natl Acad Sci USA 103:18054–18059PubMedCrossRefGoogle Scholar
  115. Sunkar R, Zhu JK (2004) Novel and stress-regulated micro RNAs and other small RNAs from Arabidopsis. Plant Cell 16:2001–2019PubMedCrossRefGoogle Scholar
  116. Takahashi H, Watanabe-Takahasi A, Smith FW, Blake-Kalf M, Hawkesford MJ, Saito K (2000) The roles of three functional sulphate transporters involved in uptake and translocation of sulphate in Arabidopsis thaliana. Plant J 23:171–182PubMedCrossRefGoogle Scholar
  117. Tang G, Galili G, Zhuang X (2007) RNAi and microRNA: breakthrough technologies for the improvement of plant nutritional value and metabolic engineering. Metabolomics 3:357–369CrossRefGoogle Scholar
  118. Tang G, Tang X, Mendu V, Tang X, Jia X, Chen Q, He L (2008) The art of microRNA: various strategies leading to gene silencing via an ancient pathway. Biochim Biophys Acta 1779:655–662PubMedGoogle Scholar
  119. Tenllado F, Martínez-García B, Vargas M, Díaz-Ruíz JR (2003) Crude extracts of bacterially expressed dsRNA can be used to protect plants against virus infections. BMC Biotechnol 3:3–14PubMedCrossRefGoogle Scholar
  120. Tester M, Langridge P (2010) Breeding technologies to increase crop production in a changing world. Science 327:818–822PubMedCrossRefGoogle Scholar
  121. Tomilov AA, Tomilova NB, Yoder JI (2007) Agrobacterium tumefaciens and Agrobacterium rhizogenes transformed roots of the parasitic plant Triphysaria versicolor retain parasitic competence. Planta 225:1059–1071PubMedCrossRefGoogle Scholar
  122. Tomilov AA, Tomilova NB, Wroblewski T, Michelmore R, Yoder JI (2008) Trans-specific gene silencing between host and parasitic plants. Plant J 56:389–397PubMedCrossRefGoogle Scholar
  123. Torres MJ, Tomilov AA, Tomilova N, Reagan RL, Yoder JI (2005) Pscroph, a parasitic plant EST database enriched for parasite associated transcripts. BMC Plant Biol 5:24PubMedCrossRefGoogle Scholar
  124. Tucker G (2003) Nutritional enhancement of plants. Curr Opin Biotechnol 14:221–225PubMedCrossRefGoogle Scholar
  125. Tyagi H, Rajasubramaniam S, Venkat Rajam M, Dasgupta I (2008) RNA-interference in rice against rice tungro bacilliform virus results in its decreased accumulation in inoculated rice plants. Transgen Res 17:897–904CrossRefGoogle Scholar
  126. Underwood BA, Tieman DM, Shibuya K, Dexter RJ, Loucas HM, Simkin AJ, Sims CA, Schmelz EA, Klee HJ, Clark DG (2005) Ethylene-regulated floral volatile synthesis in petunia corollas. Plant Physiol 138:255–266PubMedCrossRefGoogle Scholar
  127. Vanderschuren H, Alder A, Zhang P, Gruissem W (2009) Dose-dependent RNAi-mediated geminivirus resistance in the tropical root crop cassava. Plant Mol Biol 64:549–557CrossRefGoogle Scholar
  128. Vanitharani R, Chellappan P, Fauquet CM (2003) Short interfering RNA-mediated interference of gene expression and viral DNA accumulation in cultured plant cells. Proc Natl Acad Sci USA 100:9632–9636PubMedCrossRefGoogle Scholar
  129. Vaucheret H (2008) Plant ARGONAUTES. Trends Plant Sci 13:350–358PubMedCrossRefGoogle Scholar
  130. Vierstra RD (2003) The ubiquitin/26S proteasome pathway, the complex last chapter in the life of many plant proteins. Trends Plant Sci 8:135–142PubMedCrossRefGoogle Scholar
  131. Vrebalov J, Pan IL, Arroyo AJM, McQuinn R, Chung M, Poole M, Rose J, Seymour G, Grandillo S, Giovannoni J, Iris VF (2009) Fleshy fruit expansion and ripening are regulated by the tomato SHATTERPROOF Gene TAGL1. Plant Cell 21:3041–3062PubMedCrossRefGoogle Scholar
  132. Wang Y, Li J (2006) Genes controlling plant architecture. Curr Opin Biotechnol 17:123–129PubMedCrossRefGoogle Scholar
  133. Wang Y, Li J (2008) Molecular basis of plant architecture. Ann Rev Plant Biol 59:253–279CrossRefGoogle Scholar
  134. Waterhouse PM, Graham WMB (1998) Virus resistance and gene silencing in plants can be induced by simultaneous expression of sense and antisense RNA. Proc Natl Acad Sci USA 95:13959–13964PubMedCrossRefGoogle Scholar
  135. Wei S, Li X, Gruber MY, Li R, Zhou R, Zebarjadi A, Hannoufa A (2009) RNAi-mediated suppression of DET1 alters the levels of carotenoids and sinapate esters in seeds of Brassica napus. J Agric Food Chem 57:5326–5333PubMedCrossRefGoogle Scholar
  136. Wolfenbarger LL, Phifer PR (2000) Biotechnology and ecology—the ecological risks and benefits of genetically engineered plants. Science 290:2088–2093PubMedCrossRefGoogle Scholar
  137. Wroblewski T, Piskurewicz U, Tomczak A, Ochoa Michelmore RW (2007) Silencing of the major family of NBS-LRR-encoding genes in lettuce results in the loss of multiple resistance specificities. Plant J 5:803–818CrossRefGoogle Scholar
  138. Xiong A, Yao Q, Peng R, Li X, Han P, Fan H (2005) Different effects on ACC oxidase gene silencing triggered by RNA interference in transgenic tomato. Plant Cell Rep 23:639–646PubMedCrossRefGoogle Scholar
  139. Xu M, Zhu L, Shou H, Wu P (2005) A PIN1 family gene, OsPIN1, involved in auxin-dependent adventitious root emergence and tillering in rice. Plant Cell Physiol 46:1674–1681PubMedCrossRefGoogle Scholar
  140. Yoder JI, Gunathilake P, Wu B, Tomilova N, Tomilov AA (2009) Engineering host resistance against parasitic weeds with RNA interference. Pest Manag Sci 65:460–466PubMedCrossRefGoogle Scholar
  141. Zamore PD, Tuschl T, Sharp PA, Bartel DP (2000) RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals. Cell 101:25–33PubMedCrossRefGoogle Scholar
  142. Zhang L, Jing F, Li F, Li M, Wang Y, Wang G, Sun X, Tang K (2009) Development of transgenic Artemisia annua (Chinese wormwood) plants with an enhanced content of artemisinin, an effective anti-malarial drug, by hairpin-RNA mediated gene silencing. Biotechnol Appl Biochem 52:199–207PubMedCrossRefGoogle Scholar
  143. Zhao MM, An DR, Zhao J, Huang G, He Z, Chen J (2006) Transiently expressed short hairpin RNA targeting 126 kDa protein of tobacco mosaic virus interferes with virus infection. Acta Biochim Biophys Sin 38:22–28PubMedCrossRefGoogle Scholar
  144. Zhao BT, Liang RQ, Ge LF, Li W, Xiao HS, Lin HX, Ruan KC, Jin YX (2007) Identification of drought-induced microRNAs in rice. Biochem Biophys Res Commun 354:585–590PubMedCrossRefGoogle Scholar
  145. Zhou H, He SJ, Cao Y, Chen T, Du B, Chu C, Zhang J, Chen S (2006) OsGLU1, a putative membrane bound endo-1, 4-b-D-glucanase from rice, affects plant internode elongation. Plant Mol Biol 60:137–151PubMedCrossRefGoogle Scholar
  146. Zuidmeer L, Goldhahn K, Rona RJ, Gislason D, Madsen C, Summers C, Sodergren E, Dahlstrom J, Lindner T, Sigurdardottir ST, McBride D, Keil T (2008) The prevalence of plant food allergies: a systematic review. J Allergy Clin Immunol 121:1210–1218PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Umesh Balkrishna Jagtap
    • 1
  • Ranjit Gajanan Gurav
    • 1
  • Vishwas Anant Bapat
    • 1
  1. 1.Department of BiotechnologyShivaji UniversityKolhapurIndia

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