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Naturwissenschaften

, Volume 95, Issue 2, pp 149–153 | Cite as

Foraging scent marks of bumblebees: footprint cues rather than pheromone signals

  • Jessica Wilms
  • Thomas EltzEmail author
Short Communication

Abstract

In their natural habitat foraging bumblebees refuse to land on and probe flowers that have been recently visited (and depleted) by themselves, conspecifics or other bees, which increases their overall rate of nectar intake. This avoidance is often based on recognition of scent marks deposited by previous visitors. While the term ‘scent mark’ implies active labelling, it is an open question whether the repellent chemicals are pheromones actively and specifically released during flower visits, or mere footprints deposited unspecifically wherever bees walk. To distinguish between the two possibilities, we presented worker bumblebees (Bombus terrestris) with three types of feeders in a laboratory experiment: unvisited control feeders, passive feeders with a corolla that the bee had walked over on its way from the nest (with unspecific footprints), and active feeders, which the bee had just visited and depleted, but which were immediately refilled with sugar–water (potentially with specific scent marks). Bumblebees rejected both active and passive feeders more frequently than unvisited controls. The rate of rejection of passive feeders was only slightly lower than that of active feeders, and this difference vanished completely when passive corollas were walked over repeatedly on the way from the nest. Thus, mere footprints were sufficient to emulate the repellent effect of an actual feeder visit. In confirmation, glass slides on which bumblebees had walked on near the nest entrance accumulated hydrocarbons (alkanes and alkenes, C23 to C31), which had previously been shown to elicit repellency in flower choice experiments. We conclude that repellent scent marks are mere footprints, which foraging bees avoid when they encounter them in a foraging context.

Keywords

Footprints Cuticular hydrocarbons Repellent Bees Olfaction Bombusterrestris 

Notes

Acknowledgements

We thank Sebastian Witjes for advice and help with the experiments. Klaus Lunau and the members of Sensory Ecology Seminar provided critical comments that improved the manuscript. The experiments comply with the current laws of Germany. This study is supported by DFG grant EL 249/4.

Supplementary material

114_2007_298_MOESM1_ESM.doc (544 kb)
ESM 1 (DOC 557 kb)

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  1. 1.Department of NeurobiologySensory Ecology Group, University of DüsseldorfDüsseldorfGermany

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