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Naturwissenschaften

, Volume 94, Issue 9, pp 781–786 | Cite as

Dynamics of sperm transfer in the ant Leptothorax gredleri

  • Angelika OppeltEmail author
  • Jürgen Heinze
Short Communication

Abstract

Mating tactics differ remarkably between and within species of social Hymenoptera (bees, wasps, ants) concerning, e.g., mating frequencies, sperm competition, and the degree of male sperm limitation. Although social Hymenoptera might, therefore, potentially be ideal model systems for testing sexual selection theory, the dynamics of mating and sperm transfer have rarely been studied in species other than social bees, and basic information needed to draw conclusions about possible sperm competition and female choice is lacking. We investigated sperm transfer in the ant Leptothorax gredleri, a species in which female sexuals attract males by “female calling.” The analysis of 38 female sexuals fixed immediately or up to 7 days after copulation with a single male each revealed that the sperm is transferred into the female bursa copulatrix embedded in a gelatinous mass, presumably a spermatophore. Sperm cells rapidly start to migrate from the tip of the spermatophore towards the spermatheca, but transfer is drastically slowed down by an extreme constriction of the spermathecal duct, through which sperm cells have to pass virtually one by one. This results in the spermatheca being filled only between one and several hours after mating. During this time, the posterior part of the spermatophore seals the junction between bursa copulatrix and spermathecal duct and prevents sperm loss. The prolonged duration of sperm transfer might allow female sexuals to chose between ejaculates and explain previously reported patterns of single paternity of the offspring of multiply mated queens.

Keywords

Reproductive biology Mating Spermatophore Spermatheca Spermathecal duct Formicidae 

Notes

Acknowledgements

We thank Birgit Lautenschläger and Maria Schiwek for their support with histology, Wolfgang Göttler for his help with 3D reconstruction, and Stefan Buchhauser for assistance in image editing. B. Baer and two anonymous referees made helpful comments on an earlier draft of the manuscript. This study was supported by Deutsche Forschungsgemeinschaft (He 1623/19).

Supplementary material

References

  1. Allard D, Gobin B, Ito F, Tsuji K, Billen J (2002) Sperm transfer in the Japanese queenless ant Diacamma sp. (Hymenoptera: Formicidae). Neth J Zool 52:77–86CrossRefGoogle Scholar
  2. Allard D, Børgesen M, van Hulle M, Bobbaers A, Billen J, Gobin B (2006) Sperm transfer during mating in the pharaoh’s ant, Monomorium pharaonis. Physiol Entomol 31:294–298CrossRefGoogle Scholar
  3. Baer B (2005) Sexual selection in Apis bees. Apidologie 36:187–200CrossRefGoogle Scholar
  4. Baer B, Boomsma JJ (2004) Male reproductive investment and queen mating frequency in fungus growing ants. Behav Ecol 15:426–432CrossRefGoogle Scholar
  5. Baer B, Boomsma JJ (2006) Mating biology of the leaf-cutting ants Atta colombica and A. cephalotes. J Morphol 267:1165–1171PubMedCrossRefGoogle Scholar
  6. Baer B, Maile R, Schmid-Hempel P, Morgan ED, Jones GR (2000) Chemistry of a mating plug in bumblebees. J Chem Ecol 26:1869–1875CrossRefGoogle Scholar
  7. Baer B, Morgan ED, Schmid-Hempel P (2001) A nonspecific fatty acid within the bumblebee mating plug prevents females from remating. Proc Natl Acad Sci USA 98:3926–3928PubMedCrossRefGoogle Scholar
  8. Baer B, Schmid-Hempel P, Hoeg JT, Boomsma JJ (2003) Sperm length, sperm storage and mating system characteristics in bumblebees. Insectes Soc 50:101–108CrossRefGoogle Scholar
  9. Boomsma JJ, Baer B, Heinze J (2005) The evolution of male traits in social insects. Annu Rev Entomol 50:395–420PubMedCrossRefGoogle Scholar
  10. Bresslau E (1905) Der Samenblasengang der Bienenkönigin. Zool Anz 29:299–325Google Scholar
  11. Buschinger A (1968) Locksterzeln begattungsbereiter ergatoider Weibchen von Harpagoxenus sublaevis Nyl. (Hymenoptera, Formicidae). Experientia 24:297PubMedCrossRefGoogle Scholar
  12. Buschinger A (1974) Experimente und Beobachtungen zur Gründung und Entwicklung neuer Sozietäten der sklavenhaltenden Ameise Harpagoxenus sublaevis (Nyl.). Insectes Soc 21:381–406CrossRefGoogle Scholar
  13. Colonello NA, Hartfelder K (2005) She’s my girl – male accessory gland products and their function in the reproductive biology of social bees. Apidologie 36:231–244CrossRefGoogle Scholar
  14. Dallai R (1975) Fine structure of the spermatheca of Apis mellifera. J Insect Physiol 21:1110–1989CrossRefGoogle Scholar
  15. Duvoisin N, Baer B, Schmid-Hempel P (1999) Sperm transfer and male competition in a bumblebee. Anim Behav 58:743–749PubMedCrossRefGoogle Scholar
  16. Gillott C (2003) Male accessory gland secretions: modulators of female reproductive physiology and behavior. Annu Rev Entomol 48:163–184PubMedCrossRefGoogle Scholar
  17. Gobin B, Ito F, Peeters C, Billen J (2006) Queen-worker differences in spermatheca reservoir of phylogenetically basal ants. Cell Tissue Res 326:169–178PubMedCrossRefGoogle Scholar
  18. Heinze J, Ortius D (1991) Social organization of Leptothorax acervorum from Alaska (Hymenoptera: Formicidae). Psyche 98:227–240CrossRefGoogle Scholar
  19. Heinze J, Lipski N, Hölldobler B (1992) Reproductive competition in colonies of the ant Leptothorax gredleri. Ethology 90:265–278CrossRefGoogle Scholar
  20. Hölldobler B, Bartz SH (1985) Sociobiology of reproduction in ants. In: Hölldobler B, Lindauer M (eds) Experimental behavioral ecology and sociobiology. Sinauer Associates, Sunderland, MA, pp 237–257Google Scholar
  21. Hölldobler B, Wilson EO (1990) The ants. Harvard University Press, Cambridge, MAGoogle Scholar
  22. Janet C (1902) Anatomie du gaster de la Myrmica rubra. Carré et Naud, ParisGoogle Scholar
  23. Koeniger G (2005) The neglected gender – males in bees. Apidologie 36:143–144CrossRefGoogle Scholar
  24. Koeniger N, Koeniger G (1991) An evolutionary approach to mating behaviour and drone copulatory organs in Apis. Apidologie 22:581–590Google Scholar
  25. Koeniger N, Koeniger G, Wongsiri S (1989) Mating and sperm transfer in Apis florea. Apidologie 20:413–418Google Scholar
  26. Koeniger G, Koeniger N, Tingek S, Kelitu A (2000) Mating flights and sperm transfer in the dwarf honeybee Apis andreniformis (Smith, 1858). Apidologie 31:301–311CrossRefGoogle Scholar
  27. Martins GF, Serrão JE (2004) Changes in the reproductive tract of Melipona quadrifasciata anthidioides (Hymenoptera: Apidae, Meliponini) queen after mating. Sociobiology 44:241–254Google Scholar
  28. Martins GF, Serrão JE, Schmidt Furieri K (2005) Notes on the spermatheca of Vespidae and Sphecidae (Hymenoptera). Sociobiology 45:119–127Google Scholar
  29. Melo GAR, Buschini MLT, Campos LAO (2001) Ovarian activation in Melipona quadrifasciata queens triggered by mating plug stimulation (Hymenoptera, Apidae). Apidologie 32:355–361CrossRefGoogle Scholar
  30. Mikheyev AS (2004) Male accessory gland size and the evolutionary transition from single to multiple mating in the fungus-gardening ants. J Insect Sci 4:37PubMedGoogle Scholar
  31. Oberstadt B, Heinze J (2003) Mating biology and population structure of the ant, Leptothorax gredleri. Insectes Soc 50:340–345CrossRefGoogle Scholar
  32. Reichardt AK, Wheeler DE (1996) Multiple mating in the ant Acromyrmex versicolor: a case of female control. Behav Ecol Sociobiol 38:219–225CrossRefGoogle Scholar
  33. Richardson KC, Jarett L, Finke EH (1960) Embedding in epoxy resins for ultrathin sectioning in electron microscopy. Stain Technol 35:313–325PubMedGoogle Scholar
  34. Robertson HG (1995) Sperm transfer in the ant Carebara vidua F Smith (Hymenoptera: Formicidae). Insectes Soc 42:411–418CrossRefGoogle Scholar
  35. Schoeters E, Billen J (2000) The importance of the spermathecal duct in bumblebees. J Insect Physiol 46:1303–1312PubMedCrossRefGoogle Scholar
  36. Schrempf A, Heinze J, Cremer S (2005) Sexual cooperation: mating increases longevity in ant queens. Curr Biol 15:267–270PubMedGoogle Scholar
  37. Simmons LW (2001) Sperm competition and its evolutionary consequences in the insects. Princeton University Press, Princeton, USAGoogle Scholar
  38. Wheeler DE, Krutzsch PH (1994) Ultrastructure of the spermatheca and its associated gland in the Crematogaster opuntiae (Hymenoptera, Formicidae). Zoomorphology 114:203–212CrossRefGoogle Scholar
  39. Woyke J (1983) Dynamics of entry of spermatozoa into the spermatheca of instrumentally inseminated queen honey bees Apis mellifera. J Apic Res 22:150–154Google Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  1. 1.Biology I, ZoologyUniversity RegensburgRegensburgGermany

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