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Naturwissenschaften

, Volume 93, Issue 1, pp 42–45 | Cite as

Metabolically active Crenarchaeota in Altamira Cave

  • Juan M. Gonzalez
  • M. Carmen Portillo
  • Cesareo Saiz-Jimenez
Short Communication

Abstract

Altamira Cave contains valuable paleolithic paintings dating back to 15,000 years. The conservation of these unique paintings is attracting increasing interest, and so, understanding microbial proliferation in Altamira Cave represents a prioritary objective. Here, we show for the first time that members of the Crenarchaeota were metabolically active components of developing microbial communities. RNA was extracted directly from the studied environment, and a number of 16S rRNA gene sequences belonging to the low-temperature Crenarchaeota were detected. Although low-temperature Crenarchaeota detected in a variety of ecosystems by using molecular techniques remain uncultured, this RNA-based study confirms an active participation of the Crenarchaeota in cave biogeochemical cycles.

Keywords

Microbial Community Archaea Archaeal Community Microbial Colonization Fusarium Solani 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

The authors acknowledge support through a contract with the Ministry of Culture, and projects BTE2002-04492, REN2002-00041, and the Ramon y Cajal program from the Spanish Ministry of Education and Science (MEC).

References

  1. 1.
    Allemand L, Bahn PG (2005) Best way to protect rock art is to leave it alone. Nature 433:800PubMedCrossRefGoogle Scholar
  2. 2.
    Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Biol Mol 215:403–410Google Scholar
  3. 3.
    Bidle KA, Kastner M, Bartlett DH (1999) A phylogenetic analysis of microbial communities associated with methane hydrate containing marine fluids and sediments in the Cascadia margin (ODP site 892B). FEMS Microbiol Lett 177:101–108PubMedCrossRefGoogle Scholar
  4. 4.
    Castellani F (2005) The film crew. Nature 433:100–101PubMedCrossRefGoogle Scholar
  5. 5.
    Cavicchioli R, Siddiqui KS, Andrews D, Sowers K (2002) Low-temperature extremophiles and their applications. Curr Opin Biotechnol 13:253–261PubMedCrossRefGoogle Scholar
  6. 6.
    Chelius M, Moore J (2004) Molecular phylogenetic analysis of Archaea and Bacteria in Wind Cave, South Dakota. Geomicrobiol J 21:123–134CrossRefGoogle Scholar
  7. 7.
    DeLong EF (1998) Everything in moderation: Archaea as ‘non-extremophiles’. Curr Opin Genet Dev 8:649–654PubMedCrossRefGoogle Scholar
  8. 8.
    Gonzalez JM, Ortiz-Martinez A, Gonzalez-delValle MA, Laiz L, Saiz-Jimenez C (2003) An efficient strategy for screening large cloned libraries of amplified 16S rDNA sequences from complex environmental communities. J Microbiol Methods 55:459–463PubMedCrossRefGoogle Scholar
  9. 9.
    Gonzalez JM, Portillo MC, Saiz-Jimenez C (2005) Multiple displacement amplification as a pre-PCR reaction to process difficult to amplify samples and low copy number sequences from natural environments. Environ Microbiol 7:1024–1028PubMedCrossRefGoogle Scholar
  10. 10.
    Groth I, Vetermann R, Schuetze B, Schumann P, Saiz-Jimenez C (1999) Actinomycetes in kartic caves of Northern Spain (Altamira and Tito Bustillo). J Microbiol Methods 36:115–122PubMedCrossRefGoogle Scholar
  11. 11.
    Hershberger KL, Barns SM, Reysenbach AL, Dawson SC, Pace NR (1996) Wide diversity of Crenarchaeota. Nature 384:420PubMedCrossRefGoogle Scholar
  12. 12.
    Holden C (2002) Cave paintings in jeopardy. Science 297:47Google Scholar
  13. 13.
    Holden C (2003) Wanted: solution for cave mold. Science 300:245Google Scholar
  14. 14.
    Ludwig W, Strunk O, Westram R, Richter L, Meier H, Kumar Y, Buchner A, Lai T, Steppi S, Jobb G, Förster W, Brettske I, Gerber S, Ginhart AW, Gross O, Grumann S, Hermann S, Jost R, König A, Liss T, Lüßmann R, May M, Nonhoff B, Reichel B, Strehlow R, Stamatakis A, Stuckmann N, Vilbig A, Lenke M, Ludwig T, Bode A, Schleifer KH (2004) ARB: a software environment for sequence data. Nucleic Acids Res 32:1363–1371PubMedCrossRefGoogle Scholar
  15. 15.
    Kasai Y, Takahata Y, Hoaki T, Watanabe K (2005) Physiological and molecular characterization of a microbial community established in unsaturated, petroleum-contaminated soil. Environ Microbiol 7:806–818PubMedCrossRefGoogle Scholar
  16. 16.
    Mills HJ, Martinez RJ, Story S, Sobecky PA (2004) Identification of members of the metabolically active microbial populations associated with Beggiatoa species mat communities from Gulf of Mexico cold-seep sediments. Appl Environ Microbiol 70:5447–5458PubMedCrossRefGoogle Scholar
  17. 17.
    Molin S, Givskov M (1999) Application of molecular tools for in situ monitoring of bacterial growth activity. Environ Microbiol 1:383–391PubMedCrossRefGoogle Scholar
  18. 18.
    Neefs JM, Van de Peer Y, Hendriks L, De Wachter R (1990) Compilation of small ribosomal subunit RNA sequences. Nucleic Acids Res 18:2237–2317PubMedCrossRefGoogle Scholar
  19. 19.
    Nicol GW, Glover LA, Prosser JI (2003) Spatial analysis of archaeal community structure in grassland soil. Appl Environ Microbiol 69:7420–7429PubMedCrossRefGoogle Scholar
  20. 20.
    Northup DE, Barns SM, Yu LE, Spilde MN, Schelble RT, Dano KE, Crossey LJ, Connolly CA, Boston PJ, Natvig DO, Dahm CN (2003) Diverse microbial communities inhabiting ferromanganese deposits in Lechuguilla and Spider Caves. Environ Microbiol 5:1071–1086PubMedCrossRefGoogle Scholar
  21. 21.
    Ochsenreiter T, Selezi D, Quaiser A, Bonch-Osmolovskaya L, Schleper C. (2003) Diversity and abundance of Crenarchaeota in terrestrial habitats studied by 16S RNA surveys and real time PCR. Environ Microbiol 5:787–797PubMedCrossRefGoogle Scholar
  22. 22.
    Schabereiter-Gurtner C, Saiz-Jimenez C, Piñar G, Lubitz W, Rölleke S (2002a) Altamira cave Paleolithic paintings harbor partly unknown bacterial communities. FEMS Microbiol Lett 211:7–11PubMedCrossRefGoogle Scholar
  23. 23.
    Schabereiter-Gurtner C, Saiz-Jimenez C, Piñar G, Lubitz W, Rölleke S (2002b) Phylogenetic 16S rRNA analysis reveals the presence of complex and partly unknown bacterial communities in Tito Bustillo cave, Spain, and on its Paleolithic paintings. Environ Microbiol 4:392–400PubMedCrossRefGoogle Scholar
  24. 24.
    Schleper C, Jurgens G, Jonuscheit M (2005) Genomic studies of uncultivated archaea. Nat Rev Microbiol 3:479–488PubMedCrossRefGoogle Scholar
  25. 25.
    Simon HM, Dodsworth JA, Goodman RM (2000) Crenarchaeota colonize terrestrial plant roots. Environ Microbiol 2:495–505PubMedCrossRefGoogle Scholar
  26. 26.
    Sliwinski MK, Goodman RM (2004) Spatial heterogeneity of crenarchaeal assemblages within mesophilic soil ecosystems as revealed by PCR-single-stranded conformation polymorphism profiling. Appl Environ Microbiol 70:1811–1820PubMedCrossRefGoogle Scholar
  27. 27.
    Strimmer K, von Haeseler A (1996) Quartet puzzling: a quartet maximum likelihood method for reconstructing tree topologies. Mol Biol Evol 13:964–969Google Scholar
  28. 28.
    Vetriani C, Reysenbach AL, Dore J (1998) Recovery and phylogenetic analysis of archaeal rRNA sequences from continental shelf sediments. FEMS Microbiol Lett 161:83–88PubMedCrossRefGoogle Scholar
  29. 29.
    Ward DM, Weller, R, Bateson MM (1990) 16S rRNA sequences reveal numerous uncultured microorganisms in a natural community. Nature 345:63–65PubMedCrossRefGoogle Scholar
  30. 30.
    Woese CR, Kandler O, Wheelis ML (1990) Towards a natural system of organisms. Proposal for the domains Archaea, Bacteria and Eukcarya. Proc Natl Acad Sci U S A 87:4576–4579PubMedCrossRefGoogle Scholar
  31. 31.
    Zimmermann J, Gonzalez JM, Ludwig W, Saiz-Jimenez C (2005) Detection and phylogenetic relationships of a highly diverse uncultured acidobacterial community on paleolithic paintings in Altamira Cave using 23S rRNA sequence analyses. Geomicrobiol J 22:379–388CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Juan M. Gonzalez
    • 1
  • M. Carmen Portillo
    • 1
  • Cesareo Saiz-Jimenez
    • 1
  1. 1.Instituto de Recursos Naturales y Agrobiologia, CSICSevillaSpain

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