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Naturwissenschaften

, Volume 92, Issue 3, pp 147–150 | Cite as

Morphology and structure of the tarsal glands of the stingless bee Melipona seminigra

  • Stefan JarauEmail author
  • Michael Hrncir
  • Ronaldo Zucchi
  • Friedrich G. Barth
Short Communication

Abstract

Footprint secretions deposited at the nest entrance or on food sources are used for chemical communication by honey bees, bumble bees, and stingless bees. The question of the glandular origin of the substances involved, however, has not been unequivocally answered yet. We investigated the morphology and structure of tarsal glands within the fifth tarsomeres of the legs of workers of Melipona seminigra in order to clarify their possible role in the secretion of footprints. The tarsal gland is a sac-like fold forming a reservoir. Its glandular tissue is composed of a unicellular layer of specialized epidermal cells, which cover the thin cuticular intima forming the reservoir. We found that the tarsal glands lack any openings to the outside and therefore conclude that they are not involved in the secretion of footprint substances. The secretion produced accumulates within the gland’s reservoir and reaches as far as into the arolium. Thus it is likely that it serves to fill and unfold the arolium during walking to increase adhesion on smooth surfaces, as is known for honey bees and weaver ants.

Keywords

Glandular Epithelium Scent Mark Tarsal Gland Borax Solution Imaginal Moult 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

We are very grateful to Sidnei Mateus for his unfailing help in handling the bees. This study was supported by grant P 14328 of the Austrian Science Foundation (FWF) to F. G. B. The work in Brazil was conducted under permit Ibama 074/00-Difas-Direc and complies with the current laws of Brazil.

References

  1. Arnhart L (1923) Das Krallenglied der Honigbiene. Arch Bienenkd 5:37–86Google Scholar
  2. Billen JPJ (1984) Morphology of the tibial gland in the ant Crematogaster scutellaris. Naturwissenschaften 71:324–325Google Scholar
  3. Billen JPJ (1986) Etude morphologique des glandes tarsales chez la guêpe Polistes annularis (L.) (Vespidae, Polistinae). Actes Coll Insect Soc 3:51–60Google Scholar
  4. Butler CG, Fletcher DJC, Watler D (1969) Nest-entrance marking with pheromones by the honeybee—Apis mellifera L., and by a wasp, Vespula vulgaris L. Anim Behav 17:142–147Google Scholar
  5. da Cruz-Landim C, Silva de Moraes RLM, Salles HC, Reginato RD (1998) Note on glands present in Meliponinae (Hymenoptera, Apidae) bees legs. Rev Bras Zool 15:159–165Google Scholar
  6. Dahl F (1885) Die Fussdrüsen der Insekten. Arch Mikroskop Anat 25:236–262Google Scholar
  7. Federle W, Brainerd EL, McMahon TA, Hölldobler B (2001) Biomechanics of the movable pretarsal adhesive organ in ants and bees. PNAS 98:6215–6220Google Scholar
  8. Goulson D, Stout JC, Langley J, Hughes WOH (2000) Identity and function of scent marks deposited by foraging bumblebees. J Chem Ecol 26:2897–2911Google Scholar
  9. Hölldobler B, Palmer JM (1989) A new tarsal gland in ants and the possible role in chemical communication. Naturwissenschaften 76:385–386Google Scholar
  10. Hölldobler B, Obermayer M, Wilson EO (1992) Communication in the primitive cryptobiotic ant Prionopelta amabilis (Hymenoptera: Formicidae). J Comp Physiol A 171:9–16Google Scholar
  11. Hrncir M, Jarau S, Zucchi R, Barth FG (2004) On the origin and properties of scent marks deposited at the food source by a stingless bee, Melipona seminigra. Apidologie 35:3–13Google Scholar
  12. Jarau S, Hrncir M, Ayasse M, Schulz C, Francke W, Zucchi R, Barth FG (2004) A stingless bee (Melipona seminigra) marks food sources with a pheromone from its claw retractor tendons. J Chem Ecol 30:793–804Google Scholar
  13. Lensky Y, Cassier P, Finkel A, Delorme-Joulie C, Levinsohn M (1985) The fine structure of the tarsal glands of the honeybee Apis mellifera L. (Hymenoptera). Cell Tissue Res 240:153–158Google Scholar
  14. Noirot C, Quennedey A (1974) Fine structure of insect epidermal glands. Annu Rev Entomol 19:61–80Google Scholar
  15. Pouvreau A (1991) Morphology and histology of tarsal glands in bumble bees of the genera Bombus, Pyrobombus, and Megabombus. Can J Zool 69:866–872Google Scholar
  16. Quennedey A (1998) Insect epidermal cells: ultra structure and morphogenesis. In: Harrison FW, Locke M (eds) Microscopic anatomy of invertebrates, vol 11A. Insecta. Wiley–Liss, New York, pp. 177–207Google Scholar
  17. Salles HC, da Cruz-Landim C (1998) Levantamento das glândulas exócrinas presentes em Camargoia nordestina Moure, 1989 (Hymenoptera, Apidae, Meliponinae). Rev Bras Entomol 41:297–302Google Scholar
  18. Schmitt U, Lübke G, Francke W (1991) Tarsal secretion marks food sources in bumblebees (Hymenoptera: Apidae). Chemoecology 2:35–40Google Scholar
  19. Stout JC, Goulson D, Allen JA (1998) Repellent scent-marking of flowers by a guild of foraging bumblebees (Bombus spp.). Behav Ecol Sociobiol 43:317–326Google Scholar
  20. Tijskens M, Ito F, Billen J (2002) Novel exocrine glands in the legs of the ponerine ant Amblyopone reclinata (Hymenoptera, Formicidae). Neth J Zool 52:69–75Google Scholar

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Stefan Jarau
    • 1
    Email author
  • Michael Hrncir
    • 2
  • Ronaldo Zucchi
    • 3
  • Friedrich G. Barth
    • 2
  1. 1.Department of Experimental EcologyUniversity of UlmUlmGermany
  2. 2.Biocentre, Institute of ZoologyUniversity of ViennaViennaAustria
  3. 3.Department of BiologyUniversity of São Paulo, FFCLRPRibeirão PretoBrazil

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