Naturwissenschaften

, Volume 91, Issue 7, pp 329–333 | Cite as

Floral CO2 emission may indicate food abundance to nectar-feeding moths

  • Pablo G. Guerenstein
  • Enrico A.Yepez
  • Joost van Haren
  • David G. Williams
  • John G. Hildebrand
Short Communication

Abstract

As part of a study of the roles of the sensory subsystem devoted to CO2 in the nectar-feeding moth Manduca sexta, we investigated CO2 release and nectar secretion by flowers of Datura wrightii, a preferred hostplant of Manduca. Datura flowers open at dusk and wilt by the following noon. During the first hours after dusk, when Manduca feeds, the flowers produce considerable amounts of nectar and emit levels of CO2 that should be detectable by moths nearby. By midnight, however, both nectar secretion and CO2 release decrease significantly. Because nectar production requires high metabolic activity, high floral CO2 emission may indicate food abundance to the moths. We suggest that hovering moths could use the florally emitted CO2 to help them assess the nectar content before attempting to feed in order to improve their foraging efficiency.

Supplementary material

Fig. S1Datura wrigthii flower dissected longitudinally, showing the spurs (canals) in the corolla tube (see Materials and Methods)

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Fig. S2 A Manduca moth hovering in front of a Datura wrightii flower (left-down corner of the photo) at a time when we were recording CO2 and nectar production from other flowers of the same and different plants (see Discussion)

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References

  1. Bogner F (1990) Sensory physiological investigation of carbon-dioxide receptors in Lepidoptera. J Insect Physiol 36:951–957CrossRefGoogle Scholar
  2. Búrquez A, Corbet SA (1991) Do flowers reabsorb nectar? Funct Ecol 5:369–379Google Scholar
  3. Casas A, Valiente-Banuet A, Rojas-Martínez A, Dávila P (1999) Reproductive biology and the process of domestication of the columnar cactus Stenocereus stellatus in central Mexico. Am J Bot 86:534–542PubMedGoogle Scholar
  4. Castellanos MC, Wilson P, Thomson JD (2002) Dynamic nectar replenishment in flowers of Penstemon (Scrophulariaceae). Am J Bot 89:111–118Google Scholar
  5. Cruden RW, Hermann SM, Peterson S (1983) Patterns of nectar production and plant-pollinator coevolution. In: Elias T, Bentley B (eds) The biology of nectaries. Columbia University Press, New York, pp 80–125Google Scholar
  6. Dobson HEM (1994) Floral volatiles in insect biology. In: Bernays EA (ed) Insect-plant interactions, vol 5. CRC, Boca Raton, pp 47–81Google Scholar
  7. Drawert F, Klisch W, Sommer G (1987) Fermentation processes. In: Prave P, Faust V, Sittig W, Sukatsch D (eds) Fundamentals of biotechnology (English translation). VCH, New York, pp 381–435Google Scholar
  8. Fraser AM, Mechaber WL, Hildebrand JG (2003) Electroantennographic and behavioral responses of the sphinx moth Manduca sexta to hostplant volatiles. J Chem Ecol 29:1813–1833CrossRefPubMedGoogle Scholar
  9. Galetto L, Bernardello L (1993) Nectar secretion pattern and removal effects in 3 species of Solanaceae. Can J Bot 71:1394–1398Google Scholar
  10. Guerenstein PG, Christensen TA, Hildebrand JG (2002) Sensory processing of environmental-CO2 information in the moth nervous system (abstract). Chem Senses 27:661–671CrossRefGoogle Scholar
  11. Herzberg M, Fischer R, Titze A (2002) Conflicting results obtained by RAPD-PCR and large-subunit rDNA sequences in determining and comparing yeast strains isolated from flowers: a comparison of two methods. Int J Syst Evol Microbiol 52:1423–1433CrossRefPubMedGoogle Scholar
  12. Hew CS, Thio YC, Wong SY, Chin TY. (1978) Rhythmic production of CO2 by tropical orchid flowers. Physiol Plant 42:226–230Google Scholar
  13. Kent KS, Harrow ID, Quartararo P, Hildebrand JG (1986) An accessory olfactory pathway in Lepidoptera: the labial pit organ and its central projections in Manduca sexta and certain other sphinx moths and silk moths. Cell Tissue Res 245:237–245PubMedGoogle Scholar
  14. Lacher V (1964) Elektrophysiologische Untersuchungen an einzelnen Rezeptoren für Geruch, Kohlendioxyd, Luftfeuchtigkeit und Temperatur auf den Antennen der Arbeitsbiene und der Drohne (Apis mellifera L.). Z Vergl Physiol 48:587–623Google Scholar
  15. LI-COR (1998) Measuring small volumes of CO2 with the LI-COR LI-6200 system (Application Note no. 121). LI-COR Biosciences, Environmental Division, LincolnGoogle Scholar
  16. Madden AH, Chamberlin FS (1945) Biology of the tobacco hornworm in the southern cigar-tobacco district. Technical Bulletin No. 896. USDA, Washington, DCGoogle Scholar
  17. Martínez del Río C, Búrquez A (1986) Nectar production and temperature-dependent pollination in Mirabilis jalapa L. Biotropica 18:28–31Google Scholar
  18. Raguso RA, Willis MA (2002) Synergy between visual and olfactory cues in nectar feeding by naive hawkmoths, Manduca sexta. Anim Behav 64:685–695CrossRefGoogle Scholar
  19. Raguso RA, Light DM, Pichersky E (1996) Electroantennogram responses of Hyles lineata (Sphingidae: Lepidoptera) to volatile compounds from Clarkia breweri (Onagraceae) and other moth-pollinated flowers. J Chem Ecol 22:1735–1766Google Scholar
  20. Stange G (1992) High-resolution measurement of atmospheric carbon dioxide concentration changes by the labial palp organ of the moth Heliothis armigera (Lepidoptera, Noctuidae). J Comp Physiol A 171:317–324Google Scholar
  21. Stange G (1997) Effects of changes in atmospheric carbon dioxide on the location of hosts by the moth, Cactoblastis cactorum. Oecologia 110:539–545CrossRefGoogle Scholar
  22. Stange G, Monro J, Stowe S, Osmond CB (1995) The CO2 sense of the moth Cactoblastis cactorum and its probable role in the biological control of the CAM plant Opuntia stricta. Oecologia 102:341–352Google Scholar
  23. Thom C, Guerenstein P, Yepez E, Mechaber W, van Haren J, Hildebrand J, Williams D (2003) CO2 emission by Datura flowers and its significance for foraging Manduca sexta moths (abstract). Chem Senses 28:551–563 (A25)CrossRefGoogle Scholar
  24. Willmott AP, Búrquez A (1996) The pollination of Merremia palmeri (Convolvulaceae): can hawk moths be trusted? Am J Bot 83:1050–1056Google Scholar
  25. Zar JH (1999) Biostatistical analysis, 4th edn. Prentice-Hall, New JerseyGoogle Scholar

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Pablo G. Guerenstein
    • 1
  • Enrico A.Yepez
    • 2
  • Joost van Haren
    • 3
  • David G. Williams
    • 2
  • John G. Hildebrand
    • 1
  1. 1.ARL Division of NeurobiologyUniversity of ArizonaTucsonUSA
  2. 2.School of Renewable Natural ResourcesUniversity of ArizonaTucsonUSA
  3. 3.Biosphere 2 Center Columbia UniversityOracleUSA
  4. 4.Southwest Watershed Research CenterUSDA-ARSTucsonUSA

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