Journal of Molecular Medicine

, Volume 95, Issue 11, pp 1203–1213 | Cite as

Far infrared promotes wound healing through activation of Notch1 signaling

  • Yung-Ho Hsu
  • Yuan-Feng Lin
  • Cheng-Hsien Chen
  • Yu-Jhe Chiu
  • Hui-Wen ChiuEmail author
Original Article


The Notch signaling pathway is critically involved in cell proliferation, differentiation, development, and homeostasis. Far infrared (FIR) has an effect that promotes wound healing. However, the underlying molecular mechanisms are unclear. In the present study, we employed in vivo and HaCaT (a human skin keratinocyte cell line) models to elucidate the role of Notch1 signaling in FIR-promoted wound healing. We found that FIR enhanced keratinocyte migration and proliferation. FIR induced the Notch1 signaling pathway in HaCaT cells and in a microarray dataset from the Gene Expression Omnibus database. We next determined the mRNA levels of NOTCH1 in paired normal and wound skin tissues derived from clinical patients using the microarray dataset and Ingenuity Pathway Analysis software. The result indicated that the Notch1/Twist1 axis plays important roles in wound healing and tissue repair. In addition, inhibiting Notch1 signaling decreased the FIR-enhanced proliferation and migration. In a full-thickness wound model in rats, the wounds healed more rapidly and the scar size was smaller in the FIR group than in the light group. Moreover, FIR could increase Notch1 and Delta1 in skin tissues. The activation of Notch1 signaling may be considered as a possible mechanism for the promoting effect of FIR on wound healing. FIR stimulates keratinocyte migration and proliferation. Notch1 in keratinocytes has an essential role in FIR-induced migration and proliferation. NOTCH1 promotes TWIST1-mediated gene expression to assist wound healing. FIR might promote skin wound healing in a rat model.

Key messages

  • FIR stimulates keratinocyte migration and proliferation.

  • Notch1 in keratinocytes has an essential role in FIR-induced migration and proliferation.

  • NOTCH1 promotes TWIST1-mediated gene expression to assist wound healing.

  • FIR might promote skin wound healing in a rat model.


Far infrared Wound healing Notch1 Migration Proliferation 



This study was supported by the Taipei Medical University-Shuang Ho Hospital (106TMU-SHH-07).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary material

109_2017_1580_MOESM1_ESM.pdf (184 kb)
ESM 1 (PDF 184 kb)


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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  1. 1.Division of Nephrology, Department of Internal Medicine, Shuang Ho HospitalTaipei Medical UniversityNew TaipeiTaiwan
  2. 2.Department of Internal Medicine, School of Medicine, College of MedicineTaipei Medical UniversityTaipeiTaiwan
  3. 3.Graduate Institute of Clinical Medicine, College of MedicineTaipei Medical UniversityTaipeiTaiwan
  4. 4.Division of Nephrology, Department of Internal Medicine, Wan Fang HospitalTaipei Medical University TaipeiTaipeiTaiwan

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