Peroxisome proliferator-activated receptor γ inhibits pulmonary hypertension targeting store-operated calcium entry
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In this study, we investigated the role of peroxisome proliferator-activated receptor γ (PPARγ) on store-operated calcium entry (SOCE) and expression of the main store-operated calcium channel (SOCCs) components, canonical transient receptor potential (TRPC) in chronic hypoxia (CH)-induced pulmonary hypertension (CHPH) rat models. Small interfering RNA (siRNA) knockdown and adenoviral overexpression strategies were constructed for loss-of-function and gain-of-function experiments. PPARγ agonist rosiglitazone attenuates the pathogenesis of CHPH and suppresses Hif-1α, TRPC1, TRPC6 expression in the distal pulmonary arteries (PA), and SOCE in freshly isolated rat distal pulmonary arterial smooth muscle cells (PASMCs). By comprehensive use of knockdown and overexpression studies, and bioinformatical analysis of the TRPC gene promoter and luciferase reporter assay, we demonstrated that PPARγ exerts roles of anti-proliferation, anti-migration, and pro-apoptosis in PASMCs, likely by inhibiting the elevated SOCE and TRPC expression. These effects were inhibited under the conditions of hypoxia or Hif-1α accumulation. We also found that under hypoxia, accumulated Hif-1α protein acts as upstream of suppressed PPARγ level; however, targeted PPARγ rescue acts as negative feedback on suppressing Hif-1α level and Hif-1α mediated signaling pathway. PPARγ inhibits CHPH by targeting SOCE and TRPC via inhibiting Hif-1α expression and signaling transduction.
Rosiglitazone protects PH by normalizing RVSP but not right ventricle hypotrophy.
PPARγ inhibits PASMCs proliferation via targeting SOCE and TRPC by suppressing Hif-1α.
PPARγ and Hif-1α share mutual inhibitory regulation in PASMCs.
PPARγ restoration might be a beneficial strategy for PH treatment.
KeywordsPulmonary hypertension PPARγ TRPC Hif-1α
We thank Drs. Qicai Liu and Bing Li for technical assistance and constructive discussion in this study.
This work was supported by NIH (R01-HL093020), National Natural Science Foundation of China (81173112, 81470246, 81170052, 81220108001), Guangdong Natural Science Foundation team (1035101200300000), Guangzhou Department of Education Yangcheng Scholarship (12A001S), Guangzhou Department of Natural Science (2014Y2-00167), and Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme (2014, W Lu), China.
Conflict of interest
JW initiated and designed the project, analyzed data, and wrote the paper; WL designed the project and edited the paper; YW performed the animal, functional, and molecular experiments; YW, JZ, XY, and JJ performed the molecular experiments; KY and LT edited the paper; YC, QJ, BZ, and XC performed the animal experiments.
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