Journal of Molecular Medicine

, Volume 89, Issue 6, pp 603–610

A peptide binding to dimerized translationally controlled tumor protein modulates allergic reactions

  • Miyoung Kim
  • Junho Chung
  • Chulhee Lee
  • Jaehoon Jung
  • Youngjoo Kwon
  • Kyunglim Lee
Original Article

DOI: 10.1007/s00109-011-0740-8

Cite this article as:
Kim, M., Chung, J., Lee, C. et al. J Mol Med (2011) 89: 603. doi:10.1007/s00109-011-0740-8

Abstract

Translationally controlled tumor protein (TCTP) is believed to be involved in a variety of inflammatory processes: secretion of histamine and cytokines such as IL-4, IL-8, IL-13, and granulocyte/macrophage colony-stimulating factor; chemoattraction for eosinophils; augmentation of B cell proliferation; and immunoglobulin production, thereby potentially regulating allergic phenomena. In a previous study, we showed that the cytokine-releasing activity of extracellular TCTP is generated only when TCTP dimerizes via the intermolecular disulfide bond of NH2-terminal truncated TCTP implying that the dimerized TCTP (dTCTP) promotes the inflammatory phenomena. Modulation of dTCTP, thus, may offer a strategy for the treatment of chronic allergic diseases. In this study, we searched for dTCTP-binding peptides (dTBPs) by screening a phage-displayed 7-mer peptide library. We identified one peptide in the library, designated as dTBP2, which showed higher affinity to dTCTP than to full-length, monomeric TCTP. dTBP2 inhibited the induction of IL-8 by dTCTP from BEAS-2B cells. dTBP2 also reduced symptom score and eosinophil infiltration in a mouse rhinitis model. This study suggests that the dTBP2 binding to dTCTP modulates the release of inflammatory mediators of dTCTP. This result may provide a rational strategy for the treatment of allergic diseases.

Keywords

Anti-allergic peptide dTCTP Eosinophil infiltration Phage-displayed 7-mer peptide Rhinitis model Peptide libraries Asthma Drug development Biochemistry 

Supplementary material

109_2011_740_MOESM1_ESM.doc (31 kb)
ESM 1(DOC 31 kb)

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Miyoung Kim
    • 1
  • Junho Chung
    • 2
  • Chulhee Lee
    • 3
  • Jaehoon Jung
    • 1
  • Youngjoo Kwon
    • 1
  • Kyunglim Lee
    • 1
  1. 1.College of Pharmacy, Center for Cell Signalling & Drug Discovery ResearchEwha Womans UniversitySeoulSouth Korea
  2. 2.College of Medicine and Cancer Research InstituteSeoul National UniversitySeoulSouth Korea
  3. 3.Department of Otorhinolaryngology–Head and Neck SurgerySeoul National University College of MedicineSeoulSouth Korea

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