Journal of Molecular Medicine

, Volume 85, Issue 10, pp 1149–1156 | Cite as

RhoC is essential for the metastasis of gastric cancer

  • Na Liu
  • Guoyun Zhang
  • Feng Bi
  • Yanglin Pan
  • Yan Xue
  • Yongquan Shi
  • Liping Yao
  • Lina Zhao
  • Yi Zheng
  • Daiming Fan
Original Article

Abstract

Rho family members are known to regulate malignant transformation and motility of cancer cells, but the clinicopathological significance of RhoC remains unclear yet in the case of gastric cancer. In this study, we evaluated the protein expression level of RhoC in gastric cancer tissues and cell lines. Results showed that only weak staining of RhoC was detected in 3 of 33 non-tumorous cases by immunohistochemistry. The expression of RhoC was significantly higher in gastric cancer tissues (23/42, 54.8%) than in non-tumorous tissues (p < 0.01). Further analysis demonstrated that RhoC had high specificity (80.0%) in detecting gastric carcinomas with metastatic potential. RhoC was positively expressed in 18 out of 20 metastases (90.0%), even higher than that in primary gastric cancer tissues. Western blot showed that RhoC was up-regulated in five different gastric cancer cell lines but not expressed in SV40-transformed immortal gastric epithelial cell GES-1. Overexpression of RhoC GTPase in GES-1 cells could produce the motile and invasive phenotype but did not alter the monolayer growth rate. To further study the functions of RhoC, we took the powerful siRNA technology to knock down the expression of RhoC in SGC7901 cells. It was shown that down-regulation of RhoC did not affect the proliferation of SGC7901 cells. However, interference of RhoC expression could inhibit migration, invasion, and anchorage-independent growth of SGC7901 cells. In conclusion, RhoC may play a very important role in the metastasis of gastric carcinoma. Therapeutic strategies targeting RhoC and RhoC-mediated pathways may be a novel approach for treating metastasis of gastric cancer.

Keywords

Rho GTPase Gastric cancer Metastasis siRNA 

Abbreviation

SiRNA

small interfering RNA

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Na Liu
    • 1
  • Guoyun Zhang
    • 1
  • Feng Bi
    • 1
  • Yanglin Pan
    • 1
  • Yan Xue
    • 1
  • Yongquan Shi
    • 1
  • Liping Yao
    • 1
  • Lina Zhao
    • 1
  • Yi Zheng
    • 2
  • Daiming Fan
    • 1
  1. 1.State Key Laboratory of Cancer Biology and Institute of Digestive Diseases, Xijing HospitalFourth Military Medical UniversityXi’anPeople’s Republic of China
  2. 2.Division of Experimental Hematology, Children’s Hospital Research FoundationUniversity of CincinnatiCincinnatiUSA

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