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Journal of Molecular Medicine

, Volume 83, Issue 11, pp 917–926 | Cite as

Clinical significance of EGFR amplification and the aberrant EGFRvIII transcript in conventionally treated astrocytic gliomas

  • Lu Liu
  • L. Magnus Bäcklund
  • Bo R. Nilsson
  • Dan Grandér
  • Koichi Ichimura
  • Helena M. Goike
  • V. Peter CollinsEmail author
Original Article

Abstract

The aim of this study was to evaluate the clinical value of assessing epidermal growth factor receptor (EGFR) amplification and the common 5′ rearrangement of EGFR resulting in the EGFRvIII transcript in astrocytic gliomas. Data from 221 tumours were correlated with patient survival. The majority of previous studies evaluated amplification alone and provided contradictory results. Amplification was analysed by a densitometry of Southern blot analysis or quantitative polymerase chain reaction (PCR). EGFR transcripts were examined by reverse transcription PCR and subsequent sequencing. A ribonuclease (RNase) protection assay was carried out on a subgroup to confirm PCR results. Amplification of EGFR was found in 41% (65/160) of glioblastomas (GBs) and 10% (4/41) of anaplastic astrocytomas (AAs). The EGFRvIII rearrangement was identified in 54% (35/65) of GBs and 75% (3/4) of AAs with amplification, as well as in 8% (8/95) of GBs and 5% (2/37) of AAs without amplification (confirmed by RNase protection assay). There were no abnormalities of the EFGR or its transcript in grade II astrocytoma (AII). We found no significant association between EGFR amplification or rearrangement, and age or survival in the 160 GB patients. We noted a tendency towards decreased survival with any EGFR abnormality in the 41 patients with AAs. This was most marked in the five cases with the EGFRvIII transcript (p=0.069), but these were significantly older than those without (p=0.023). No abnormalities of EGFR were identified in AII patients. We conclude that neither EGFR amplification nor the presence of the EGFRvIII transcript predicts patient outcome in conventionally treated GBs. However, in AAs, although uncommon, EGFR aberrations appear to be associated with shorter survival.

Keywords

EGFRvIII Survival Transcript Glioblastoma 

Notes

Acknowledgements

We thank the Departments of Neurosurgery and Pathology at the Karolinska University Hospital, Sahlgrenska University Hospital, and many other hospitals in Sweden that helped us obtain follow-up data.

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Lu Liu
    • 1
  • L. Magnus Bäcklund
    • 2
  • Bo R. Nilsson
    • 2
  • Dan Grandér
    • 2
  • Koichi Ichimura
    • 1
  • Helena M. Goike
    • 2
  • V. Peter Collins
    • 1
    Email author
  1. 1.Division of Molecular Histopathology, Department of Pathology, Addenbrooke’s HospitalUniversity of CambridgeCambridgeUK
  2. 2.Department of Oncology–PathologyKarolinska University HospitalStockholmSweden

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