Journal of Molecular Medicine

, Volume 83, Issue 10, pp 812–821 | Cite as

A two-dimensional electrophoresis reference map of human ovary

  • Lei Wang
  • Ye-Fei Zhu
  • Xue-Jiang Guo
  • Ran Huo
  • Xiang Ma
  • Min Lin
  • Zuo-Min Zhou
  • Jia-Hao Sha
Original Article

Abstract

The ovary plays a central role in oogenesis and gonadal hormone secretion. Proteomic analysis is a valuable approach for gaining an increased understanding of the molecular nature of the ovary. In this work, two-dimensional electrophoresis for protein separation followed by matrix-assisted laser desorption/ionization mass spectrometry and database searches, identified 231 protein spots corresponding to 138 individual proteins that were found in gels representing both the follicular and luteal phases. The data were used to construct a database online (http://reprod.njmu.edu.cn/2d). The identified proteins were functionally classified into seven groups: (1) cell signaling/communication, (2) cell division, (3) gene/protein expression, (4) metabolism, (5) cell structure and motility, (6) cell/organism defense, and (7) unclassified. Among the proteins identified, 47% had not been previously reported in the human ovary. In addition, a number of disease-related proteins were identified in this protein map, including some cancer- and polycystic ovarian syndrome-related proteins. Two proteins with phosphorylation were verified by Western blot analysis. Comparison of protein abundance between follicular and luteal stages produced seven protein spots that had been identified in our database. This study provides a preliminary reference map of normal human ovary that will form a basis for comparative studies on normal and pathological conditions of the human ovary and may serve as a potential tool for clinical diagnosis, therapeutics, and prognosis.

Keywords

Human ovary Two-dimensional electrophoresis Reference map Proteomics 

Abbreviations

PCOS

polycystic ovarian syndrome

2-D PAGE

two-dimensional polyacrylamide gel electrophoresis

CHAPS

3-[(3-cholamidopropyl)-dimethylammonio]-1-propane sulfonate

IPG

immobilized pH gradient

SDS

sodium dodecyl sulphate

APS

ammonium persulfate

TEMED

N,N,N′,N′-tetramethylethylenediamine

ACN

acetonitrile

TFA

trifluoroacetic acid

DTT

dithiothreitol

hnRNP A2/B1

heterogeneous nuclear ribonucleoproteins A2/B1

IEF

Isoelectric focusing

MALDI-TOF

matrix-assisted laser desorption/ionization time-of-flight

pI

isoelectric points

MWs

molecular weights

ANX

annexin

HCC

hepatocellular carcinoma

VDAC

voltage-dependent anion-selective channel

BAK

BCL2 homologous antagonist/killer

Supplementary material

109_2005_676_ESM_supp.pdf (96 kb)
(PDF 96 KB)

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Lei Wang
    • 1
  • Ye-Fei Zhu
    • 1
  • Xue-Jiang Guo
    • 1
  • Ran Huo
    • 1
  • Xiang Ma
    • 1
  • Min Lin
    • 1
  • Zuo-Min Zhou
    • 1
  • Jia-Hao Sha
    • 1
  1. 1.Laboratory of Reproductive MedicineNanjing Medical UniversityNanjingPeople’s Republic of China

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