Journal of Molecular Medicine

, Volume 83, Issue 5, pp 362–376 | Cite as

Gene expression profiling: cell cycle deregulation and aneuploidy do not cause breast cancer formation in WAP-SVT/t transgenic animals

  • Andreas Klein
  • Eva Guhl
  • Raphael Zollinger
  • Yin-Jeh Tzeng
  • Ralf Wessel
  • Michael Hummel
  • Monika Graessmann
  • Adolf Graessmann
Original Article

Abstract

Microarray studies revealed that as a first hit the SV40 T/t antigen causes deregulation of 462 genes in mammary gland cells (ME cells) of WAP-SVT/t transgenic animals. The majority of deregulated genes are cell proliferation specific and Rb-E2F dependent, causing ME cell proliferation and gland hyperplasia but not breast cancer formation. In the breast tumor cells a further 207 genes are differentially expressed, most of them belonging to the cell communication category. In tissue culture breast tumor cells frequently switch off WAP-SVT/t transgene expression and regain the morphology and growth characteristics of normal ME cells, although the tumor-revertant cells are aneuploid and only 114 genes regain the expression level of normal ME cells. The profile of retransformants shows that only 38 deregulated genes are tumor-specific, and that none of them is considered to be a typical breast cancer gene.

Keywords

Breast cancer Mammary gland epithelial cells SV40 T/t antigen Tumorigenesis WAP-SVT/t transgene 

Abbreviations

CA

Correspondence analysis

DMEM

Dulcecco’s modified Eagle medium

IRF

Interferon regulatory factor

ME

Mammary gland epithelial

OAS

2′-5′-Oligoadenylate synthetase family members

PBS

Phosphate-buffered saline

SV40

Simian virus 40

WAP

Whey acidic milk protein

Notes

Acknowledgements

This work was supported by the Verband der Chemischen Industrie. We thank Dr. A. Corfield for critical reading of the manuscript.

Supplementary material

Supplement 1 462 differentially expressed genes from normal to transgenic animals (first hit)

Supplement_1.pdf (93 kb)
(PDF 93 KB)

Supplement 2 207 differentially expressed genes from transgenic to tumor animals (second hit)

Supplement_2.pdf (58 kb)
(PDF 58 KB)

Supplement 3 114 differentially expressed genes from breast tumor to Revertant-ME-B cells

Supplement_3.pdf (94 kb)
(PDF 95 KB)

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Andreas Klein
    • 1
  • Eva Guhl
    • 1
  • Raphael Zollinger
    • 2
  • Yin-Jeh Tzeng
    • 3
  • Ralf Wessel
    • 1
  • Michael Hummel
    • 2
  • Monika Graessmann
    • 1
  • Adolf Graessmann
    • 1
  1. 1.Institut für Molekularbiologie und Bioinformatik, Charité Hospital, Campus Benjamin FranklinUniversitätsmedizin BerlinBerlinGermany
  2. 2.Institut für Pathologie, Charité Hospital, Campus Benjamin FranklinUniversitätsmedizin BerlinBerlinGermany
  3. 3.Graduate Institute of Molecular and Cell BiologyTzu-Chi UniversityHualienTaiwan

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