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Journal of Molecular Medicine

, Volume 82, Issue 5, pp 317–324 | Cite as

A spermatogenesis-related gene expression profile in human spermatozoa and its potential clinical applications

  • Hui Wang
  • Zuomin Zhou
  • Min Xu
  • Jianmin Li
  • Junhua Xiao
  • Zhi-Yang Xu
  • Jiahao ShaEmail author
Original Article

Abstract

Spermatogenesis is an essential stage in the human reproductive process. In a previously study aiming to determine which genes might be involved in spermatogenesis, we compared the gene expression profiles of adult and fetal testes by hybridizing cDNA probes prepared from adult and fetal testes to membranes dotted with gene clones derived from a commercial human testis library. We identified 266 differentially expressed genes that showed higher expression levels in adult testes, indicating their potential roles in spermatogenesis. In the present study, we applied the same cDNA microarray technique to the analysis of gene expression in the spermatozoa of normal fertile men and found 149 genes that were expressed at higher levels in adult testis. A further study of five sperm motility-related genes selected from this profile by real-time PCR revealed that there was significant difference in the expression levels of two genes (TPX-1, testis-specific protein 1 and LDHC, lactate dehydrogenase C, transcript variant 1) between normal (n=29) and motility impaired (n=24) semen samples, indicating that these genes are involved in sperm function. Our results demonstrated that spermatogenesis-related gene profiling could help to assess sperm quality in humans, and further study of these genes will help us to elucidate the mechanisms involved in spermatogenesis and diseases relating to human infertility.

Keywords

cDNA microarray Gene expression mRNA Spermatozoa 

Notes

Acknowledgements

This research was supported by grants from National 973 Plan of China (G1999055901), Nature Science Foundation of China (30170485) and the Foundation of Science and Technology of Jiangsu Province, China (BG2003028). We thank Dr. Aimin Tang for his critical review of the manuscript.

Supplementary material

A list of the 149 reported genes. Genes are classified based on their functions and arranged by their hybridization signal intensities in microarray

supp.pdf (48 kb)
(PDF 48 KB)

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Hui Wang
    • 1
  • Zuomin Zhou
    • 1
  • Min Xu
    • 1
  • Jianmin Li
    • 1
  • Junhua Xiao
    • 1
  • Zhi-Yang Xu
    • 1
  • Jiahao Sha
    • 1
    Email author
  1. 1.Key Laboratory of Reproductive MedicineNanjing Medical UniversityNanjing China

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